(A) (Left) Viral strategy shown for transsynaptic labeling of direct inputs to PAG-USV neurons (performed in N = 4 males). (Right) Confocal images are shown of starter PAG-USV neurons, upstream …
Source data for Figure 1A–C.
Source data for Figure 1D.
(A) Example confocal images showing transsynaptic labeling of neurons in the preoptic area of the hypothalamus (preoptic area [POA], green) that provide monosynaptic input to GABAergic …
(A) Example confocal images showing transsynaptic labeling of neurons in the infralimbic cortex (green) that provide monosynaptic input to GABAergic periaqueductal gray (PAG) neurons (left-most …
(A) Example confocal images showing transsynaptic labeling of neurons in the anterior hypothalamus (green) that provide monosynaptic input to GABAergic periaqueductal gray (PAG) neurons (left-most …
(A) Example confocal images showing transsynaptic labeling of neurons in the ventral pallidum (green) that provide monosynaptic input to GABAergic periaqueductal gray (PAG) neurons (left-most …
(A) (Left) Viral strategy to express ChR2 in POAPAG neurons. (Right) Example trial showing that optogenetic activation of POAPAG neurons elicits USV production in an isolated animal. (B) (Left) …
Source data for Figure 2C and F.
(A) Mean USV rate across trials aligned to delivery of blue light pulses plotted for N = 8 individual mice following optogenetic activation of POAPAG neurons. Plots represent the individual animals …
Source data for panels B and C of Figure 2—figure supplement 1.
(A) Real-time place preference tests were performed in which either POA or AmgC/M neurons were optogenetically activated when mice were in one of two sides of a test chamber (see Materials and method…
Source data for panel A of Figure 2—figure supplement 2.
POAPAG neurons are labeled with GFP, and AmgC/M-PAG neurons are labeled with tdTomato. (Top left) Plane of section through the preoptic area (POA) shows the beginning of POAPAG cell body labeling in …
(A) The variational autoencoder (VAE) takes spectrograms as input (left), maps the spectrograms to low-dimensional latent representations using an encoder (middle), and approximately reconstructs …
Source data for Figure 3D.
(A) (Left) Viral strategy used to express ChR2 in AmgC/M-PAG neurons. (Right) Confocal image of representative AmgC/M-PAG cell body labeling achieved with this viral strategy. (B) (Left) Spectrogram …
Source data for Figure 4C and D.
AmgC/M-PAG neurons were labeled by injecting AAV-retro-Cre into the caudolateral periaqueductal gray (PAG) and AAV-FLEX-GFP into the AmgC/M. (A–C) Three planes of coronal section are shown that …
AAV-retro-Cre injection into the caudolateral PAG. Representative coronal sections are shown for POA and amygdala cell body labeling observed after monosynaptic rabies-based tracing from GABAergic …
(A) Viral strategy (left) and schematic (right) for whole-cell patch clamp recordings from fluorescently identified CANE-tagged PAG-USV neurons while optogenetically activating AmgC/M-PAG axons. (B) …
Source data for Figure 5D and F.
(A) Viral strategy (left) and schematic (right) for whole-cell patch clamp recordings from fluorescently identified VGAT+ PAG cells while optogenetically activating POAPAG axons. (B) Example image …
Source data for Figure 6D and H.
(A) Viral strategy (left) and schematic (right) for whole-cell patch clamp recordings from fluorescently identified PAG-USV cells while optogenetically activating POAPAG axons. (B) Light-evoked …
Source data for panel B of Figure 6—figure supplement 1.
Inhibitory neurons within the POA provide direct input to inhibitory neurons within the PAG, which in turn provide direct input to PAG-USV neurons. In this manner, activation of POAPAG neurons …
An isolated male mouse is shown which has ChR2 is expressed in POAPAG neurons. Optogenetic activation of these neurons with pulses of blue light elicits USV production. Video is shown at the top, a …
An example male mouse with ChR2 expression in AmgC/M-PAG neurons is shown alone in a chamber with no social partner. Optogenetic activation of AmgC/M-PAG neurons with pulses of blue light does not …
An example male mouse with ChR2 expression in AmgC/M-PAG neurons is shown alone in a chamber with no social partner. Optogenetic activation of AmgC/M-PAG neurons with pulses of blue light does not …
An example male mouse with ChR2 expression in AmgC/M-PAG neurons is shown alone in a chamber with no social partner. Optogenetic activation of AmgC/M-PAG neurons with pulses of blue light does not …
A male mouse which has ChR2 expressed in AmgC/M-PAG neurons is shown interacting with and producing USVs directed at a female social partner. Optogenetic activation of these neurons with pulses of …
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background (Mus musculus, C57BL/6J) | C57 | Jackson Labs | RRID:IMSR_JAX:000664 | |
Strain, strain background (Mus musculus, B6N.129S6(Cg)-Esr1tm1.1(cre)And/J) | Esr1-Cre | Jackson Labs | RRID:IMSR_JAX:017911 | |
Strain, strain background (Mus musculus, B6J.129S6(FVB)-Slc32a1tm2(cre)Lowl/ MwarJ) | VGAT-Cre | Jackson Labs | RRID:IMSR_JAX:016962 | |
Strain, strain background (Mus musculus, B6;129S6-Gt(ROSA)26Sortim14(Cag-tdTomato)Hze/J) | Ai14 | Jackson Labs | RRID:IMSR_JAX:007908 | |
Strain, strain background (Mus musculus, B6;129-Fostm1.1Fawa/J) | Fos-dsTVA | Jackson Labs | RRID:IMSR_JAX:027831 | |
Recombinant DNA reagent | AAV2/1-hSyn-Flex-Chr2-eYFP | Addgene (K. Deisseroth) | RRID:Addgene_26973 | |
Recombinant DNA reagent | AAV-pgk-retro-Cre | Addgene (P. Aebischer) | RRID:Addgene_24593 | |
Recombinant DNA reagent | AAV2/1-pCAG-flex-GFP | Addgene (H. Zeng) | RRID:Addgene_51502 | |
Recombinant DNA reagent | AAV2/1-pCAG-flex-Tdtomato | Addgene (H. Zeng) | RRID:Addgene_51503 | |
Recombinant DNA reagent | AAV-flex-oG | Duke Viral Vector Core | ||
Recombinant DNA reagent | EnvA-G-RV-GFP | Rodriguez et al., 2017 (DOI: 10.1038/s41593-017-0012-1), Sakurai et al., 2016 (DOI: 10.1016/j.neuron.2016.10.015) | ||
Recombinant DNA reagent | CANE-RV-mCherry | Rodriguez et al., 2017 (DOI: 10.1038/s41593-017-0012-1), Sakurai et al., 2016 (DOI: 10.1016/j.neuron.2016.10.015) | ||
Recombinant DNA reagent | AAV-flex-TVA-mCherry | Rodriguez et al., 2017 (DOI: 10.1038/s41593-017-0012-1), Sakurai et al., 2016 (DOI: 10.1016/j.neuron.2016.10.015) | ||
Commercial assay or kit | HCR v3.0 | Molecular Instruments | ||
Chemical compound, drug | Gabazine | Tocris | Cat# 1262 | (10 µM) |
Chemical compound, drug | TTX | Tocris | Cat# 1069 | (2 µM) |
Chemical compound, drug | 4AP | Sigma-Aldrich | Cat# 275875 | (100 µM) |
Software, algorithm | MATLAB | Mathworks | RRID:SCR_001622 | |
Software, algorithm | ImageJ | NIH | RRID:SCR_003070 | |
Software, algorithm | ZEN | Zeiss | RRID:SCR_013672 | |
Software, algorithm | Spike7 | CED | RRID:SCR_000903 | |
Software, algorithm | pClamp | Molecular Devices | RRID:SCR_011323 | |
Software, algorithm | IGOR Pro | WaveMetrics | RRID:SCR_000325 | |
Other | NeuroTrace 435/455 | Invitrogen/Thermo Fischer Scientific | Cat# N21479 | (1:500) |