To lie or not to lie: Super-relaxing with myosins
- Department of Biology, MyoKardia Inc, United States
- Department of Biochemistry, Stanford University School of Medicine, United States
Figures
Figure 1
Schematic representation of the possible different functional states of myosin.
(a) Highlighted in green are actin-bound myosins, which are most active and utilize maximum ATP. This myosin state is in equilibrium with an off-actin state, shown in orange, the DRX state of myosin, which has 100-fold less activity than the actin-interacting form. Myosins in the DRX state are in equilibrium with those in the SRX state, as shown in red, which has a further 10-fold less activity than the DRX state. The SRX state of myosin could be due to either a traditional IHM state or a non-IHM state. Actin is shown as gray globular domains, and the myosin thick-filament shaft is shown as a gray cylindrical rod. (b) Simulated energy (ATP) utilization by different myosin states is shown. The ATP turnover rate for the actin-bound myosin, DRX myosin, and SRX myosin was assumed to be 3 s−1, 0.03 s−1, and 0.003 s−1, respectively. A standard deviation of 0.1 units was used to generate random Gaussian noise. The simulated curves were generated using the GraphPad Prism software.
Figure 2
Schematic representation of the possible different functional states of myosin.
(a) Different states of myosins are arranged in the order of their energy utilization- actin-bound myosin in green, DRX myosin in orange, and SRX myosin in red. (b) Graphical representation of how different physiological, pathophysiological, and non-physiological perturbations, as listed, alter the myosin population in these three different states. The small blue arrows next to the different perturbations denote an increase of those parameters. The battery symbol in each box qualitatively resembles the energy saved by the system in each scenario.
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To lie or not to lie: Super-relaxing with myosins
eLife 10:e63703.
https://doi.org/10.7554/eLife.63703
