Related to main text Figure 3. Wildtype male C57BL/6J mice were fed chow or chow supplemented with the choline TMA lyase inhibitor iodomethylcholine (IMC) for 7 days. Mice were then necropsied at 4 hr intervals to collect blood and metabolic tissues (liver and gastrocnemius skeletal muscle). (a) Plasma levels of choline, TMA, and trimethylamine N-oxide (TMAO). (b) The relative mRNA expression of forkhead box protein O1 (Foxo1), choline kinase α (Chkα), phosphatidylethanolamine N-methyltransferase (Pemt) in the liver over a 24 hr period. (c) The relative mRNA expression of the TMA receptor trace amine-associated receptor 5 (Taar5), nuclear receptor subfamily one group D member 1 (Nr1d1; RevErbα), and peroxisome proliferator-activated receptor gamma coactivator 1α (Pgc1α) in the gastrocnemius muscle over a 24 hr period. (d) Circadian parameters (MESOR, amplitude, and acrophase) were determined by cosinor analysis with a 24 hr period in plasma, liver, and skeletal muscle. The overall fit for each model is shown in cosinor detect, and a Wald multivariate test was used to compare circadian parameters between groups. All data represent mean ± SEM (n = 7–8 per group).