(A) Kaplan–Meier plot for orthotopic xenografts grouped by GSC state. For each of the 37 GSCs used in the xenograft survival analysis, the median survival value was used from multiple mice injected with cells from each GSC (average number of mice injected/GSC = 5). The dotted lines indicate median survival. The pairwise p-values are also significant for Invasive vs Reactive (p=0.02) and Invasive vs Constructive (p=0.045) but not for Reactive vs Constructive (p=0.45). (B) Invaded area over time normalized to t0 by three to four representative GSCs of each GSC state and human fetal neural stem cells (hfNSCs) used as controls. See Figure 5—figure supplement 1A for individual GSC invasion. (C) Invasion images of representative hfNSCs and GSCs at t0, 6 hr, and 12 hr. Scale bar is 150 µm. (D) TCGA samples ordered by increasing concordance with Invasive GSCs and grouped into three subgroups: <1, 1–1.65, >1.65. (E) Kaplan–Meier plot for TCGA samples grouped by concordance with Invasive GSCs. The dotted line indicates median survival. When considering pairwise comparisons, only the Invasive-high and Invasive-low subgroups were significantly different (p=0.0043). Further subgrouping of the TCGA samples into smaller intervals of concordance z-score yielded no benefit, preserving the Invasive-high subgroup as the only one with significantly poorer prognosis (Figure 5—figure supplement 2B,C).