(A) Distributions of the membrane contact area of RND1 and RhoD bound with the plexin dimer. The data was from three simulations each of the RND1- and RhoD-bound dimers; each system contained two RND1 or RhoD molecules, hence the six sets of data. The compiled data are shown in Figure 3D. (B) The membrane contact area or RND1 or RhoD from the three simulations each of the RND1- and RhoD-bound dimers; each system contained two RND1 or RhoD molecules, hence the six sets of data, respectively. The initial positions of RND1 were inherited from the end of a RhoD-bound plexin dimer simulation, with extensive RND1-membrane contact (the dashed line). Conversely, the initial positions of RhoD were inherited from the end of an RND1-bound plexin dimer, with limited RhoD-membrane contact (the dashed line). As shown, the RND1 membrane contact area generally decreased while the RhoD membrane contract area increased in the simulations. (C) The membrane contact area data (B) shown in violin plots. The left and the right regulatory GTPases are shown separately. The left panel is shown in Figure 3I and here again for completeness. (D) RND1/RhoD-membrane contact map in a simulation of RND1-bound plexin dimer and another of RhoD-bound plexin dimer. The lower panels show the residue contact maps, and the upper panel show the total numbers of residues as functions of time. (E) 1-Palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS) (negatively charged) lipids (red) are enriched at the RhoD interface (left panels); the percentage of POPS relative to all lipids in the interface is consistently higher than the 30% average (right panel). This is a plexin dimer system with two RhoD molecules, but membrane imprint for only one is shown because the other RhoD molecule bears much less membrane contact. (F) POPS (red) and phosphatidylinositol (4,5)-bisphosphate (PIP2) (green) charged lipids are enriched at the RhoD interfaces (left panels) in simulations. The number of lipid charges in interaction with RhoD increased in the simulation (right panel).