(A) Top five canonical pathways subject to transcriptional alterations in the liver identified by ingenuity pathway analysis of RNA-seq of sham versus CLP mice (n = 4 mice per group). Blue …
(A) GSEA of the ROS metabolic pathway (GO:0072593) from RNA-seq from livers comparing sham to CLP showing a positive enrichment score (NES = 1.117) (n = 4 mice per group). (B–F) Relative metabolite …
Ultrahigh-performance liquid chromatography–tandem mass spectroscopy measurement of homocysteine from isolated hepatocytes 30 hr post-surgery. n = 5 sham, n = 8 CLP.
(A) Top five induced and repressed physiological pathways in the liver of sham versus CLP mice identified by IPA of RNA-seq. (B) GSEA of the fatty acid metabolic pathway (GO:0019395) from RNA-seq …
(A) Heatmap representation of average log2 fold change of genes involved in fatty acid oxidation (GO:0019395) and fatty acid biosynthetic process (GO:0006633) in CLP versus sham operated mice (n = 5 …
(A) Volcano plot of significantly altered lipids from sham and CLP hepatocytes measured by UPLC–MS/MS (n = 5 sham; n = 8 CLP). (B) Pie chart of lipid compositions from sham and CLP mice. (C) …
Ultrahigh-performance liquid chromatography–tandem mass spectroscopy measurement of (A) dihydroceramide, (B) ceramide, (C) hexosylceramide, and (D) lactosylceramide from isolated hepatocytes 30 hr …
Ultrahigh-performance liquid chromatography–tandem mass spectroscopy measurement of (A) free fatty acid species, (B) phosphatidylcholine species, (C) phosphatidylethanolamine species, and (D) …
Ultrahigh-performance liquid chromatography–tandem mass spectroscopy measurement of (A) phosphatidylinositol, (B) lysophosphatidylethanolamine species, and (C) cholesterol ester from isolated …
Lipidomic analysis.
Metabolomic analysis.
Transcriptomic analysis.