(a) Representation of lymph gland anterior and posterior lobes from third instar larvae. The anterior lobe is composed of progenitors (red) and core progenitors (hatched red), and the cortical zone …
Results of the RNAi ligand screen RNAi was expressed in cardiac cells by using the handΔ-gal4 and/or NP1029-gal4 driver.
Crystal cells were labeled by BcGFP, PSC cells were immune-stained with Antp antibody, and to visualize the core progenitors tep4 in situ hybridization was performed. In most cases, 2 RNAi lines were tested per ligand, and at least 15 lymph glands per RNAi were analyzed. Crystal cell index and PSC cell number were established. The green and red colored boxes indicate an increase and a decrease, respectively, compared to the control. Black dashes indicate that no difference was observed compared to the control. A white box indicates that this condition was not tested. Most RNAi lines that gave a modification in crystal cell index with the handΔ-gal4 driver were also analyzed with another cardiac cell driver NP1029-gal4, and proPO antibody immunostainings were performed to visualize crystal cells. Finally, for all RNAi lines that led to a defect in crystal cell differentiation with the handΔ-gal4 driver, tep4 in situ hybridizations were performed and the tep4 index was established.
Results of the RNAi ligand screen.
(a–c’) HandΔ-gal4 > mcd8 GFP is green in (a–c) and white in (a’–c’). (d–f’) NP1029-gal4 > mcd8 GFP is green in (d–f) and white in (d’–f’). The PSC is labeled by Col (red and arrow in a); thin and …
RNAi screen quantification data.
(a) A maximum projection of 5 confocal lymph gland sections, bnl (red) is expressed in cardiac cells and MZ progenitors that express domeMESO-GFP (green). (a’, a’’) An enlarged view, bnl is red (a’) …
Quantification of Figure 2.
(a–c”) A maximum projection of 5 confocal lymph gland sections, bnl is in red. (a,b,c) and white in (a”,b”,c”). BcGFP (green) labels crystal cells. (a’–a”) An enlarged view, crystal cells are green …
Quantification of Figure 2—figure supplement 1.
(a, b) Crystal cells are labeled by proPO antibody (green). An increase in crystal cell number is observed when bnl expression is decreased in MZ progenitors (b) compared to the control (a). (c) …
Quantification of Figure 2—figure supplement 2.
(a) A maximum projection of 5 confocal lymph gland sections of larvae expressing btl:cherryendo (red) and domeMESO-GFP that labels MZ progenitors (green). (a’, a’’) An enlarged view, btl:cherryendo …
Quantification of Figure 3.
(a–c”) A maximum projection of 5 confocal lymph gland sections of larvae expressing btl:cherryendo (red). (a–a’) BcGFP (green) labels crystal cells. (a’–a”) An enlarged view, crystal cells are green …
Quantification of Figure 3—figure supplement 1.
(a) Active bnl::GFP fusion protein is expressed in cardiac cells using handΔ-gal4 driver. Dashed lines indicate cardiac tube and the PSC is labeled by Collier (Col, red and red arrow). (a’, a’’) An …
Quantification of Figure 4.
(a–b, d–e) PSC cells are labeled with Antp (green) antibody. An increase in PSC cell numbers is observed when sar1 is knocked down in cardiac cells using the handΔ driver (b) compared to the control …
Quantification of Figure 4—figure supplement 1.
(a, b) GCaMP3 Ca2+ sensor (dome >UAS-GCaMP3) is white. GCaMP3 intensity decreases when btl is knocked down in MZ progenitors (b) compared to the control (a). (c) Quantification of GCaMP3 intensity. …
Quantification of Figure 5.
(a, b) L2 larval lymph glands and the GCaMP3 Ca2+ sensor (dome >UAS-GCaMP3) is white. No difference observed in GCaMP3 intensity between the control (a) and when btl is knocked down in MZ …
Quantification of Figure 5—figure supplement 1.