(A) Probability distribution of active (red) and passive (blue) vertex displacements, referred to as the van Hove function (see Equation 4 in Appendix Section 2), shows distinct non-Gaussian functional form. Inset shows deviation of the van Hove function from fits to Gaussian function for the active vertex and for the passive vertex. Probability distribution of distances moved over a time scale of , clearly shows the enhanced distance moved by active vertices as compared to passive vertices. Probability distribution is obtained for 20 individual vertices from 10 embryos. (B) Van Hove distribution of active (red) and passive (blue) vertex displacements at . Van Hove distribution for both active and passive vertices are well fit by a Gaussian distribution indicative of normal diffusive movement at short time scales. (C) Average vertex speed distribution for active (red) and passive (blue) vertices at (Appendix, Section 2). The speed distribution peaks at low values of average speed and rapidly decays of zero, showing similar trends for both active and passive vertices at . (D) Average vertex speed distribution for active (red) and passive (blue) vertices at . The distribution peaks at intermediate values of average speed. The active vertex speed distribution decays slower for larger values of the average velocity as compared to passive vertices. This is indicative of enhanced movement of active vertices. (E) Velocity autocorrelation function (VACF) for active (red) and passive (blue) vertices at (Appendix, Section 2). Active and passive vertex velocity autocorrelation rapidly decay to zero over a time scale of ~. Individual vertex correlations are plotted as solid lines. Mean is plotted as dashed lines. Active and vertex velocity correlations are similar in time at a short time interval, . (F) Velocity autocorrelation function (VACF) for active (red) and passive (blue) vertices at (Appendix, Section 2). Active vertex velocity is more persistent in time at this longer time interval compared to the passive vertex velocity. VACF quantifies the emergence of persistent motion of the active vertex at . The persistence time of the velocity correlation is over . (G) The self-overlap parameter, as quantified by decays slower for passive vertices (blue) as compared to active vertices (red). (H) For non-shortening vertices (magenta, black), the overlap parameter decays slower compared to active vertices (red). The overlap decays to 0 in the time interval probed for active vertices. The decay in the self-overlap parameter for passive vertices are comparable to non-shortening left and right vertices. See Appendix, Section 3 for further details. (I) The four-point susceptibility, , is calculated from the moments of with peaks visible for both active and passive vertices. (J) for non-shortening left (black) and right (magenta) vertices do not show a peak in the time frame analyzed. See Appendix, Section 3 for further details on the calculation of four-point susceptibility.