(A) A four cell T1 transition with mediolaterally (ML)-aligned ‘v-junctions’ (red) and anterior-posterior (A/P) aligned t-junctions (orange) indicated. (B) Frames from time-lapse showing vertex …
(A) Cartoon depiction of Xenopus gastrulation, tadpole axis elongation, and the consequence of convergent extension defects. Here the mesoderm in the dorsal marginal zone (DMZ) involutes and …
Appendix, Section 1. (A) Schematic showing how vertex MSD was measured from the lab frame of reference. Here the coordinate plane was set with the upper left corner of the image as (x,y)(0,0) and …
(A) Probability distribution of active (red) and passive (blue) vertex displacements, referred to as the van Hove function (see Equation 4 in Appendix Section 2), shows distinct non-Gaussian …
(A) Sketch of v- junction shortening with elements of the model overlain. Active (red) and passive (blue) vertex movements are affected by a piston modulating the dynamic rest length. The vertices …
(A) C. Normalized relative change in length, , versus time for shortening mediolateral cell-cell junctions during CE. 21 individual junctions from 20 embryos are analyzed. and are the …
(A) Schematic of transverse fluctuations in the vertex position perpendicular to the direction of junction shortening; traverse movements are extracted using the transverse 'hop' function, which is …
(A) C-cadherin (Cdh3) cis-clustering; trans-dimers form across opposing cell membranes (gray); lateral cis interactions drive clustering. (B) Frames from time-lapse of Cdh3-GFP; white arrows …
(A) Schematic showing how actin was measured next to active and passive vertices at shortening v-junctions. (B) Graph showing junction length and actin intensities over time for a single shortening …
(A-J) Individual (time frame by frame) spatial correlation vs distance curves selecting for shortening events from 10 distinct cell-cell junctions that undergo successful junction shortening …
(A) Mutations used to inhibit cadherin cis-clustering. (B) Cdh3-GFP clustering in a control embryo. (C) Cis-clusters absent after re-expression of cisMut-Cdh3-GFP. (D) Mean spatial autocorrelation …
(A) Embryos were injected with Cdh3-MO and membrane-BFP in a single dorsal blastomere at the four-cell stage resulting in mosaic depletion of Cdh3. Here, immuno-staining for Cdh3 shows that the …
(A) Image of polarized, elongated control Xenopus mesoderm cells. Blue = mediolateral (ML); yellow = anterior-posterior (AP). (B) Stage-matched cells after depletion of endogenous Cdh3 and …
(A) Cartoon of polarized core PCP protein localization. (B) Still image of Cdh3-GFP after expression of dominant negative Dvl2 (Xdd1). (C) Spatial autocorrelation of Cdh3 intensity fluctuations for …
(A) Spatial autocorrelation of Cdh3 intensity fluctuations for wild type (60 frames, obtained from 10 embryos) and Cdh3-rescue (58 frames, obtained from 4 embryos). The characteristic correlation …