(A) Average frequencies of Ca2+ events (A) for baseline and cue-enriched conditions recorded during quiet immobility (rest, dark bars) and running (run, light bars). Data from n = 9 mice, last baseline session and cue-enriched session for panels A and B (2-way ANOVA, run vs. rest: F(1,32) = 1.71, p=0.20, baseline vs cue-enriched F(1,32) = 1.80, p=0.19). (B), Same as A for the magnitude of Ca2+ transients (2-way ANOVA, run vs. rest: F(1,32) = 1.67, p=0.21, baseline vs cue-enriched F(1,32) < 0.01, p=0.99) (C), Distribution of event frequencies for all cells for the cue-enriched condition. (D) Under cue-enriched conditions, network events also occurred mainly during immobility. Mean number of network events per recording session during running (light green) and resting (dark green). Gray bars depict shuffled data for each condition. Section sign indicates ANOVA F(3,28) = 8.6, p=0.0003, asterisk indicates Bonferroni post-test resting vs. shuffled p=0.0019. (E) Cos-similarity between network events under cue-enriched conditions. Similarity of population vectors computed for individual network events. Comparisons were carried out between all possible pairwise combinations of vectors and quantified using cosine similarity. The number of orthogonal network events were significantly higher than expected by chance (Comparison shuffled vs. real data, Wilcoxon signed rank test p=0.031). (F–J) Activity of MPP inputs in the cue-enriched condition. Data from n = 4 mice, one mouse included additionally to Figure 1F that had only jRGECO expression in MPP, but no granule cell signal for panels G-J. Shaded areas indicate standard errors. (F) Mean fluorescence averaged during resting (dark red) and running (light red, n = 4) in cue-enriched trials. ANOVA for running vs. immobile states F(1,3) = 9.64, p=0.02. (G) Variance of MPP bulk signal during resting (dark red) and running (light red, n = 4) in cue-enriched trials. ANOVA for running vs. Immobile states n.s. (H) Cross correlation of MPP bulk signal and summed GC signal during resting. Gray shaded area indicates standard error. (I) In cue-enriched conditions, MPP activity also increases at transitions from immobility to running (red line, MPP activity, blue line indicates running speed, n = 4). (J) Average MPP activity (red) and probability of granule cells being active in cue-enriched trials, both aligned to the time point of network events. Red shaded areas indicate standard error.