Abstract

Epidermal keratinocytes mediate touch sensation by detecting and encoding tactile information to sensory neurons. However, the specific mechanotransducers that enable keratinocytes to respond to mechanical stimulation are unknown. Here, we found that the mechanically-gated ion channel PIEZO1 is a key keratinocyte mechanotransducer. Keratinocyte expression of PIEZO1 is critical for normal sensory afferent firing and behavioral responses to mechanical stimuli in mice.

Data availability

All data generated or analyzed during this study are included in the manuscript and supporting files.

Article and author information

Author details

  1. Alexander R Mikesell

    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Wauwatosa, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Olena Isaeva

    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Francie Moehring

    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-0071-5685
  4. Katelyn E Sadler

    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-2078-3527
  5. Anthony D Menzel

    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, United States
    Competing interests
    The authors declare that no competing interests exist.
  6. Cheryl L Stucky

    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, United States
    For correspondence
    cstucky@mcw.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-4966-6594

Funding

National Institute of Neurological Disorders and Stroke (NS040538)

  • Cheryl L Stucky

National Institute of Neurological Disorders and Stroke (NS070711)

  • Cheryl L Stucky

National Institute of Neurological Disorders and Stroke (NS108278)

  • Cheryl L Stucky

Medical College of Wisconsin (Advancing a Healthier Wisconsin Endowment)

  • Cheryl L Stucky

National Institute of Neurological Disorders and Stroke (1F31NS125941-01)

  • Alexander R Mikesell

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Alexander Theodore Chesler, National Institutes of Health, United States

Ethics

Animal experimentation: Animal experimentation: All protocols were in accordance with National Institutes of Health guidelines and were approved by the Institutional Animal Care and Use Committee at the Medical College of Wisconsin (Milwaukee, WI; protocol #383).

Version history

  1. Preprint posted: July 19, 2020 (view preprint)
  2. Received: December 22, 2020
  3. Accepted: September 1, 2022
  4. Accepted Manuscript published: September 2, 2022 (version 1)
  5. Version of Record published: September 26, 2022 (version 2)

Copyright

© 2022, Mikesell et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Alexander R Mikesell
  2. Olena Isaeva
  3. Francie Moehring
  4. Katelyn E Sadler
  5. Anthony D Menzel
  6. Cheryl L Stucky
(2022)
Keratinocyte PIEZO1 modulates cutaneous mechanosensation
eLife 11:e65987.
https://doi.org/10.7554/eLife.65987

Share this article

https://doi.org/10.7554/eLife.65987

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