(A) Classification of steroid receptor binding relative to genomic features expressed as percentage (%) of peaks after 60 min of hormone treatment inside each feature. Legend at the top right corner indicates the color key for ERbs (green dots) and three conditions of PR binding: non-pretreated with E2 (PRbs, red dots), pretreated with E2 for 12 hr (prePRbs, blue dots) and exogenous expression of PR (FPRbs, grey dots). The table below shows percentages represented in the plot. (B) To the left: Representation of GREAT tool association rules adapted with modifications (McLean et al., 2010). To the right: Venn diagrams show intersection between PRbs-associated genes and R5020-regulated genes (top), and ERbs-associated genes and E2-regulated genes (bottom). (C) Peak signals in PRbs, FPRbs and prePRbs from 60 min R5020-treated Ishikawa cells were plotted as heatmaps. Regions were defined inside a window of 10 kb centered in peak summit (±5 kb) and intensity of the signal correspond to number of reads in each region. Heatmap is subdivided into four mutually exclusive groups depending on shared/partly shared/non-shared binding sites: a (n = 1342), sites shared by all three conditions of PR binding; b (n = 1072), sites uniquely found in FPR and prePR; c (n = 633), sites found only in FPR; and d (n = 3162), sites found only in prePR. De novo motif discovery (MEME) was performed on all groups and results are indicated as sequence logos to the right of the map, including the name of the most related known motif. PRE: progesterone response element. (D) Peak signals in PRbs, FPRbs and prePRbs as in (C), and ERbs from 60 min E2-treated Ishikawa cells. Heatmap was subdivided into five mutually exclusive groups: e (n = 198), sites shared by all three conditions of PR binding and ER binding; f (n = 112), sites shared by FPRbs, prePRbs and ERbs; g (n = 24), sites shared by FPRbs and ERbs; h (n = 329), sites shared by prePRbs and ERbs; and i (n = 918), sites uniquely found in ERbs. Motif discovery was performed as in A for all groups and results are shown to the right of the map, including the most related known motif. ERE: estrogen response element; 1/2 ERE: half ERE.