(A) A conserved motif is common to all six heat shock proteins (HSPs) mRNAs. MEME analysis of the sequences of all six mRNAs was performed and revealed three conserved motifs in the coding regions, shown schematically as sequence logo based on nucleotide representation. (B) Distribution of the motifs over the HSP genes. Motif location is plotted, along with their p-values over the different genes. Motifs 1–3 correspond to those shown in (A), respectively. (C) Single gene histone deletions do not affect cell growth after heat shock. The doubling times (minutes) of WT, hhf1Δ, hhf2Δ, hta1∆, hta2∆, htb1∆, htb2∆, hht1∆, and hht2∆ strains were assessed for cells grown on a liquid-rich medium (YPD) exposed to heat shock (50°C; 60 min;+HS) or not (-HS) and then allowed to recover for 18 hr at 30°C. Error bars represent the avg. ± sd of four biological repeats. A one-way ANOVA with multiple comparisons with and without heat shock for each strain was performed to calculate the statistical significance; p<0.0001 for all comparisons. (D) Combined hhf2∆ and AID-HHF1 alleles result in increased doubling time upon auxin induction and heat shock. MATa wild-type (WT) and hhf2∆ AID-HHF1 cells were grown to mid-log phase, treated either with or without auxin (3-IAA; 4 mM) for 3.5 hr, and exposed to heat shock (50°C; 0, 15, 30, 60 min; HS). After removal to a fresh YPD medium lacking auxin, strains were grown for 18 hr at 30°C and the doubling times assessed. Three biological replicates were performed and a one-way ANOVA test with multiple comparisons was performed to calculate the statistical significance between time points; ****p-value<0.0001. ns – non-significant.