The left column represents example sleep histories for the VC L2/3 experiments while right column shows mEPSC characteristics for the indicated cell types and brain regions. (A) Top figure, hypnograms for three animals used for sleep-dense mEPSC recordings. Green bars indicate instances of wake, magenta indicates rapid eye movement (REM), and blue non-rapid eye movement (NREM). Bottom, this same behavioral state data represented as a moving average of % time spent awake in the preceding 4 hr; the dashed lines represent the thresholds for achieving the required density in a given state, and the dots represent endpoints for slice recordings. (B) and (C) are same as for (A), but for wake-dense recordings performed during the light and dark periods, respectively. Red arrow indicates wake encouragement (introduction of toy). Black circle around endpoint dot indicates which trace is represented by example hypnogram. (D) Traces on the left, example mEPSC recordings from L2/3 of primary visual cortex (V1); scale bar, 10 pA, 1 s. On the right, cell average mEPSC events; scale bar, 5 pA, 5 ms. (E) On left, cell average mEPSC amplitudes from L2/3 V1; error bars = 95% CI of mean (p=0.17; Kruskal-Wallis). (E) On right, cumulative histogram of sampled mEPSC amplitudes (SD – L. WD p=0.075, L. WD – D. WD p>0.9, SD – D. WD p=0.77; Kolmogorov-Smirnov (KS) test with Bonferroni correction). Inset for (E)–(G) is a peak scaled average mEPSC waveform; scale bar, 50% peak, 5 ms. Sleep dense n = 17 cells, three animals; L. wake dense n = 25 cells, four animals; D. wake dense n = 18 cells, five animals. (F) On left, cell average mEPSC from L4 V1 (p=0.52; Kruskal-Wallis). (F) On right, cumulative mEPSC amp. from L4 V1 (p=0.99; KS test). Sleep dense n = 47 cells, six animals; L. wake dense n = 32 cells, four animals. (G) On left, cell average mEPSC amplitudes from L2/3 prefrontal cortex (PFC) (p=0.13; Kruskal-Wallis). (G) On right, cumulative histogram of sampled mEPSC amplitudes (SD – L. WD p>0.9, L. WD – D. WD p=0.096, SD – D. WD p=0.015; KS test with Bonferroni correction). Sleep dense n = 31 cells, six animals; L. wake dense n = 28 cells, five animals; D. wake dense n = 18 cells, six animals.