Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism
- Universidad de Chile, Chile
- Institute of Science and Technology Austria (IST Austria), Austria
- Institute of Science and Technology Austria, Austria
Abstract
The developmental strategies used by progenitor cells to allow a safe journey from their induction place towards the site of terminal differentiation are still poorly understood. Here we uncovered a mechanism of progenitor cell allocation that stems from an incomplete process of epithelial delamination that allows progenitors to coordinate their movement with adjacent extra-embryonic tissues. Progenitors of the zebrafish laterality organ originate from the superficial epithelial enveloping layer by an apical constriction process of cell delamination. During this process, progenitors retain long-lasting apical contacts that enable the epithelial layer to pull a subset of progenitors on their way to the vegetal pole. The remaining delaminated cells follow the movement of apically attached progenitors by a protrusion-dependent cell-cell contact mechanism, avoiding sequestration by the adjacent endoderm, ensuring their collective fate and allocation at the site of differentiation. Thus, we reveal that incomplete delamination serves as a cellular platform for coordinated tissue movements during development.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files. Source data files have been provided for Figures 1, 2, 3, 4, 5, 6, 7 and 8.
Article and author information
Author details
Carl-Philipp Heisenberg
Funding
Fondo Nacional de Desarrollo Científico y Tecnológico (1190806)
- Eduardo Pulgar
- Steffen Härtel
- Miguel Concha
Comisión Nacional de Investigación Científica y Tecnológica (REDES170212,REDES130020)
- Eduardo Pulgar
- Steffen Härtel
- Miguel Concha
H2020 European Research Council (Advanced grant 742573)
- Carl-Philipp Heisenberg
Fondo Nacional de Desarrollo Científico y Tecnológico (1161274,1181823)
- Steffen Härtel
- Miguel Concha
Instituto Milenio de Neurociencia Biomedica (ICN09_015)
- Eduardo Pulgar
- Steffen Härtel
- Miguel Concha
Millennium Nucleus Physics of Active Matter from ANID (ANID)
- Susana Márquez
- Rodrigo Soto
- Miguel Concha
Fondo de Equipamiento Cientifico y Tecnologico (EQM130051)
- Steffen Härtel
- Miguel Concha
Fondo de Financiamiento de Centros de Investigacion en Areas Prioritarias (15150012)
- Miguel Concha
Fondo Nacional de Desarrollo Científico y Tecnológico (3160478)
- Eduardo Pulgar
Comisión Nacional de Investigación Científica y Tecnológica (PIA ACT-1402)
- Steffen Härtel
- Miguel Concha
Comisión Nacional de Investigación Científica y Tecnológica (PIA ACT192015)
- Miguel Concha
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: Fish care and procedures were approved by the Ethical Review Committee and comply with the Animals Scientific Procedures Act 0466 (Protocol CBA#0466 FMUCH).
Copyright
© 2021, Pulgar et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism
eLife 10:e66483.
https://doi.org/10.7554/eLife.66483
