(A) Schematic of mRNA injections and subsequent imaged regions of the Xenopus tropicalis embryo. (B) Cell segmentation and tracking workflow. Binary segmentation, cell surface tracking, and cell …
(A) Raw data for apical area (converted to square microns) of an individual cell over time. (B) Apical area averaged/smoothed over 7 frames. (C) Smoothed data after mean-centering and …
(A) Overall change (Δ) in apical surface area (standardized) across anterior (left) and posterior (right) control embryos. (B) Overall change in medial LifeAct/actin localization (standardized) …
Tissue-level cell size and protein localization dynamics from control embryos in Figure 2. (X) Distribution of overall change (Δ) in displayed parameter (standardized) among cells from control …
Xenopus tropicalis embryos were fixed in paraformaldehyde then stained with the monoclonal N-cadherin antibody mNCD-2. Increased medial N-cadherin localization is apparent as cells constrict.
(A) XY (top row) and XZ (bottom row) projections of N-cadherin-GFP and LifeAct-RFP in the anterior neural ectoderm of a Xenopus tropicalis embryo. (B) XY (top panel) and XZ (bottom panel) …
Three time points of GFP-Shroom3ΔC-term and LifeAct-RFP in constricting neural ectoderm cells of a Xenopus embryo.
(A) Schematic of mosaic F0 CRISPR/Cas9 injections in Xenopus tropicalis embryos. (B) Workflow of identification and analysis of mosaic F0 crispants. (C) Top row, distribution of initial area (square …
(A) Gene model for shroom3 in Xenopus tropicalis per Xenbase. The sgRNA was designed to target the 5’ end of the second exon. (B) Fragment analysis to validate efficacy of sgRNA+ Cas9 injections. A …
(A) Smoothed mean of medial LifeAct-RFP/actin fluorescent intensity across all imaged cells across time. (B) Smoothed mean of medial N-cadherin-GFP fluorescent intensity across all imaged cells …
(A) Representative images of LifeAct/actin and N-cadherin-GFP (N-cad-GFP) localization in control cells (left) and shroom3 crispant cells (right) from the anterior region of the neural ectoderm. …
(A-D) 2D density plots of all observations of apical area versus medial (A) or junctional (B) LifeAct/actin or medial (C) or junctional (D) N-cadherin for all cells within each group. Percentages in …
(A and B) 2D density plots of all observations of medial (A) or junctional (B) LifeAct/actin versus apical area for all cells within each group. (C and D) 2D density plots of all observations of …
(A) Representative images of LifeAct/actin and N-cadherin-GFP (N-cad-GFP) localization in control cells (left) and shroom3 crispant cells (right) from the posterior region of the neural ectoderm. …
(A and B) 2D density plots of all observations of apical area versus medial (A) or junctional (B) LifeAct/actin for all cells within each group. (C and D) 2D density plots of all observations of …
(A) Junction orientation from posterior control embryo from Figure 2. Scale bars = 100 µm. (B) Distribution of overall change (Δ) in junction length (standardized) from the posterior neural …
(A) Distribution of overall change (Δ) in junction length (standardized) from the anterior neural ectoderm. Horizontal lines on density plots/violins indicate quartiles of distribution, black …