TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis

Abstract

TRPM7 contributes to a variety of physiological and pathological processes in many tissues and cells. With a widespread distribution in the nervous system, TRPM7 is involved in animal behaviors and neuronal death induced by ischemia. However, the physiological role of TRPM7 in CNS neuron remains unclear. Here, we identify endocytic defects in neuroendocrine cells and neurons from TRPM7 knockout (KO) mice, indicating a role of TRPM7 in synaptic vesicle endocytosis. Our experiments further pinpoint the importance of TRPM7 as an ion channel in synaptic vesicle endocytosis. Ca2+ imaging detects a defect in presynaptic Ca2+ dynamics in TRPM7 KO neuron, suggesting an importance of Ca2+ influx via TRPM7 in synaptic vesicle endocytosis. Moreover, the short-term depression is enhanced in both excitatory and inhibitory synaptic transmission from TRPM7 KO mice. Taken together, our data suggests that Ca2+ influx via TRPM7 may be critical for short-term plasticity of synaptic strength by regulating synaptic vesicle endocytosis in neurons.

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All data generated or analyzed during this study are included in the manuscript and supporting files.

Article and author information

Author details

  1. Zhong-Jiao Jiang

    Biological Sciences, University of Illinois at Chicago, Chicago, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. Wenping Li

    Biological Sciences, University of Illinois at Chicago, Chicago, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Li-Hua Yao

    Biological Sciences, University of Illinois at Chicago, Chicago, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. Badeia Saed

    Biological Sciences, University of Illinois at Chicago, Chicago, United States
    Competing interests
    The authors declare that no competing interests exist.
  5. Yan Rao

    Biological Sciences, University of Illinois at Chicago, Chicago, United States
    Competing interests
    The authors declare that no competing interests exist.
  6. Brian S Grewe

    Biological Sciences, University of Illinois at Chicago, Chicago, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-8287-6588
  7. Andrea McGinley

    Biological Sciences, University of Illinois at Chicago, Chicago, United States
    Competing interests
    The authors declare that no competing interests exist.
  8. Kelly Varga

    Biological Sciences, University of Illinois at Chicago, Chicago, United States
    Competing interests
    The authors declare that no competing interests exist.
  9. Simon Alford

    Biological Sciences, University of Illinois at Chicago, Chicago, United States
    Competing interests
    The authors declare that no competing interests exist.
  10. Ying S Hu

    University of Illinois at Chicago, Chicgo, United States
    Competing interests
    The authors declare that no competing interests exist.
  11. Liang-Wei Gong

    Biological Sciences, University of Illinois at Chicago, Chicago, United States
    For correspondence
    lwgong@uic.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-5868-6039

Funding

NIH Office of the Director (R01NS110533)

  • Liang-Wei Gong

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: All animal experimental studies were approved by the Animal Care and Use Committee of the University of Illinois at Chicago and conformed to the guidelines of the National Institutes of Health.(animal protocol number 19-189).

Reviewing Editor

  1. Nils Brose, Max Planck Institute of Experimental Medicine, Germany

Version history

  1. Received: January 20, 2021
  2. Preprint posted: January 25, 2021 (view preprint)
  3. Accepted: September 10, 2021
  4. Accepted Manuscript published: September 27, 2021 (version 1)
  5. Version of Record published: October 14, 2021 (version 2)

Copyright

© 2021, Jiang et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Zhong-Jiao Jiang
  2. Wenping Li
  3. Li-Hua Yao
  4. Badeia Saed
  5. Yan Rao
  6. Brian S Grewe
  7. Andrea McGinley
  8. Kelly Varga
  9. Simon Alford
  10. Ying S Hu
  11. Liang-Wei Gong
(2021)
TRPM7 is critical for short-term synaptic depression by regulating synaptic vesicle endocytosis
eLife 10:e66709.
https://doi.org/10.7554/eLife.66709

Share this article

https://doi.org/10.7554/eLife.66709

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