Strain, strain background (Mus musculus, male) | BALB/cAJcl-nu/nu | CLEA | | Ten-week-old |
Cell line (Homo sapiens) | Lenti-X 293T | Takara Bio Inc | | |
Cell line (Homo sapiens) | HeLaS3 | K.Matsumoto (Nagoya University, Aichi, Japan) | | RRID:CVCL_0058 |
Cell line (Homo sapiens) | S2-CP8 | Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University | | RRID:CVCL_F971 |
Cell line (Homo sapiens) | PANC-1 | RIKEN Bioresource Center Cell Bank | | RRID:CVCL_0480 |
Cell line (Homo sapiens) | BxPC-3 | American Type Culture Collection | | RRID:CVCL_0186 |
Cell line (Homo sapiens) | HPAF-II | American Type Culture Collection | | RRID:CVCL_0313 |
Transfected construct (Homo sapiens) | ARL4C-EGFP | This paper | | Various mutants of ARL4C |
Transfected construct (Homo sapiens) | ARL4CG2A-EGFP | This paper | | Various mutants of ARL4C |
Transfected construct (Homo sapiens) | ARL4CT27N-EGFP | This paper | | Various mutants of ARL4C |
Transfected construct (Homo sapiens) | ARL4CQ72L-EGFP | This paper | | Various mutants of ARL4C |
Transfected construct (Homo sapiens) | ARL4CΔPBR-EGFP | This paper | | Various mutants of ARL4C |
Transfected construct (Homo sapiens) | ARL4CG2A-EGFP-Cterm | This paper | | Various mutants of ARL4C |
Transfected construct (Homo sapiens) | ARL4CΔPBR-EGFP-Cterm | This paper | | Various mutants of ARL4C |
Transfected construct (Homo sapiens) | ARL4CG2A-EGFP-GRP1PH | This paper | | Various mutants of ARL4C |
Transfected construct (Homo sapiens) | ARL4CG2A-EGFP-PLCδPH | This paper | | Various mutants of ARL4C |
Transfected construct (Homo sapiens) | ARL4C-mCherry | This paper | | Various mutants of ARL4C |
Transfected construct (Homo sapiens) | ARL4C-tdTomato | This paper | | Various mutants of ARL4C |
Transfected construct (Homo sapiens) | ARL4C-FLAG-HA | This paper | | Various mutants of ARL4C |
Biological sample (Homo sapiens) | Resected specimens of 57 patients with PDAC | Osaka University | | |
Antibody | ARL4C (rabbit polyclonal) | Atlas Antibodies | #HPA028927 | (WB 1:1000, IHC 1:50) |
Antibody | Clathrin (mouse monoclonal) | BD Biosciences | #610,500 | (WB 1:1000) |
Antibody | EGR1 (rabbit monoclonal) | Cell Signaling Technology | #4,153 S | (WB 1:1000) |
Antibody | β-catenin (mouse monoclonal) | BD Biosciences | #610,154 | (WB 1:1000) |
Antibody | Ras (G12D) (rabbit monoclonal) | Cell Signaling Technology | #14,429 S | (WB 1:1000) |
Antibody | Hsp90 (mouse monoclonal) | BD Biosciences | #610,419 | (WB 1:1000) |
Antibody | HA (mouse monoclonal) | BioLegend | #901,502 | (WB 1:1000) |
Antibody | HA (rat monoclonal) | Roche | #1867423001 | (ICC 1:100) |
Antibody | GFP (rabbit polyclonal) | Life Technologies/Thermo Fisher Scientific | #A6455 | (WB 1:4000) |
Antibody | GFP (mouse monoclonal) | Santa Cruz Santa Cruz Biotechnology | #sc-9996 | (WB 1:1000) |
Antibody | FLAG (mouse monoclonal) | WAKO | #014–22,383 | (WB 1:1000) |
Antibody | IQGAP1 (mouse monoclonal) | Santa Cruz Santa Cruz Biotechnology | #sc-376021 | (WB 1:1000, IHC 1:800, ICC 1:100) |
Antibody | MMP14 (rabbit monoclonal) | Abcam | #ab51074 | (WB 1:1000, IHC 1:200, ICC 1:100) |
Antibody | Cytohesin2 (rabbit polyclonal) | Proteintech Group, Inc | #67185–1-Ig | (ICC 1:100) |
Antibody | Rac1 (mouse monoclonal) | BD Biosciences | #610,651 | (WB 1:1000) |
Antibody | Cdc42 (rabbit polyclonal) | Cell Signaling Technology | #2,466 S | (WB 1:1000) |
Antibody | CK19 (rabbit monoclonal) | Abcam | #ab52625 | (IHC 1:100) |
Antibody | Mitochondria (mouse monoclonal) | Merck Millipore | #MAB1273 | (IHC 1:100) |
Antibody | LYVE-1 (rabbit polyclonal) | Abcam | #ab14917 | (IHC 1:100) |
Antibody | YAP/TAZ (rabbit monoclonal) | Cell Signaling Technology | #8,418 S | (ICC 1:100) |
Antibody | Paxillin (mouse monoclonal) | BD Biosciences | #610,052 | (ICC 1:100) |
Antibody | FAK(mouse monoclonal) | BD Biosciences | #610,087 | (ICC 1:100) |
Antibody | P-Paxillin (Y118)(rabbit polyclonal) | Cell Signaling Technology | #2,541 S | (ICC 1:100) |
Antibody | P-FAK (Y397)(rabbit monoclonal) | Life Technologies/Thermo Fisher Scientific | #44,625 G | (ICC 1:100) |
Antibody | Cortactin(mouse monoclonal) | Merck Millipore | #05–180 | (ICC 1:100) |
Antibody | ARL4C (rabbit polyclonal) | This paper | SAJ5550275 | (ICC 1:100) |
Recombinant DNA reagent | pEGFPC2-IQGAP1 (plasmid) | K.Kaibuchi (Nagoya University, Japan) | | |
Recombinant DNA reagent | pEGFP-mCyth2 (plasmid) | J.Yamauchi (Tokyo University of Pharmacy and Life Science, Japan), | | |
Recombinant DNA reagent | pAcGFP-mPlcd1PH (plasmid) | M.Matsuda (Kyoto University, Kyoto, Japan) | | |
Recombinant DNA reagent | CSII-CMV-MCS-IRES2-Bsd (plasmid) | H.Miyoshi (RIKEN Bioresource Center, Ibaraki, Japan) | | |
Recombinant DNA reagent | pEGFPN3-hARL4C (plasmid) | A.Kikuchi (Osaka University, Osaka, Japan) | | |
Recombinant DNA reagent | pEGFPN3-hGRP1 (plasmid) | This paper | | Full length cDNAs of GRP1 ORF were reversely transcribed from mRNA extracted from MCF-7 cells. |
Recombinant DNA reagent | pEGFPN3-hMMP14 (plasmid) | This paper | | Full length cDNAs of MMP14 ORF were reversely transcribed from mRNA extracted from U2OS cells. |
Recombinant DNA reagent | mRFP-FKBP-5-ptase-dom | Addgene | 67,516 | |
Recombinant DNA reagent | PM-FRB-CFP | Addgene | 67,517 | |
Sequence-based reagent | siRNA: randomized control | This paper | | 5'-CAGTCGCGTTTGCGACTGG-3' |
Sequence-based reagent | siRNA: human IQGAP1#1 | This paper | | 5'-GCTGCACATAGTTGCCTTT-3' |
Sequence-based reagent | siRNA: human IQGAP1#2 | This paper | | 5'-CCCTAATGTAGAATGTCAT-3' |
Sequence-based reagent | siRNA: human CYTH2#1 | This paper | | 5'-GGATGGAGCTGGAGAACAT-3' |
Sequence-based reagent | siRNA: human CYTH2#2 | This paper | | 5'-GCAGTTTCTATGGAGCTTT-3' |
Sequence-based reagent | siRNA: human ARPC2#1 | This paper | | 5'-GCCTATATTCACACACGTA-3' |
Sequence-based reagent | siRNA: human ARPC2#2 | This paper | | 5'-CCTATATTCACACACGTAT-3' |
Sequence-based reagent | siRNA: human MMP14#1 | This paper | | 5'-GCAGCCTCTCACTACTCTT-3' |
Sequence-based reagent | siRNA: human MMP14#2 | This paper | | 5'-CCGACATCATGATCTTCTT-3' |
Sequence-based reagent | siRNA: human KRAS#1 | This paper | | 5'-GCATCATGTCCTATAGTTT-3' |
Sequence-based reagent | siRNA: human KRAS#2 | This paper | | 5'-GTTGGAGCTGATGGCGTAG-3' |
Sequence-based reagent | siRNA: human CTNNB1#1 | This paper | | 5'-CCCACTAATGTCCAGCGTT-3' |
Sequence-based reagent | siRNA: human CTNNB1#2 | This paper | | 5'-GCATAACCTTTCCCATCAT-3' |
Sequence-based reagent | Antisense oligonucleotide: randomized control | This paper | | T(Y)^a^g^A(Y)^g^a^G(Y)^t^a^5(Y)^c^c^A(Y)^t^c (Lower case = DNA; N(Y) = AmNA; 5(Y) = AmNA_mC; ^ = Phosphorothioated) |
Sequence-based reagent | Antisense oligonucleotide: ARL4C-1316 | This paper | | G(Y)^5(Y)^A(Y)^t^a^c^c^t^c^a^g^g^T(Y)^A(Y)^a (Lower case = DNA; N(Y) = AmNA; 5(Y) = AmNA_mC; ^ = Phosphorothioated) |
Sequence-based reagent | human GAPDH_F | This paper | PCR primers | 5'-TCCTGCACCACCAACTGCTT-3' |
Sequence-based reagent | human GAPDH_R | This paper | PCR primers | 5'-TGGCAGTGATGGCATGGAC-3' |
Sequence-based reagent | human B2M_F | This paper | PCR primers | 5'-TGCTGTCTCCATGTTTGATGTATC-3 |
Sequence-based reagent | human B2M_R | This paper | PCR primers | 5'-TCTCTGCTCCCCACCTCTAAG-3' |
Sequence-based reagent | human ARL4C_F | This paper | PCR primers | 5'-AGGGGCTGTGAAGCTGAGTA-3’ |
Sequence-based reagent | human ARL4C_R | This paper | PCR primers | 5'-TTCCAGGCTGAAAAGCAGTT –3' |
Sequence-based reagent | human ARPC2_F | This paper | PCR primers | 5'-AGATTTCGATGGGGTCCTCT-3' |
Sequence-based reagent | human ARPC2_R | This paper | PCR primers | 5'-CCGGAAGATTTTCAAGGTCA-3' |
peptide, recombinant protein | FLAG peptide | Sigma-Aldrich | F3290 | |
Commercial assay or kit | Lipofectamine2000 transfection reagent | Life Technologies/Thermo Fisher Scientific | 11668019 | |
Commercial assay or kit | Lipofectamine LTX reagent | Life Technologies/Thermo Fisher Scientific | 15338100 | |
Commercial assay or kit | RNAiMAX | Life Technologies/Thermo Fisher Scientific | 13778075 | |
Commercial assay or kit | ViaFect | Promega Corp. | E4981 | |
Commercial assay or kit | TrypLE Express Enzyme | Thermo Fisher Scientific | 12604013 | |
Commercial assay or kit | PrimeSTAR Max DNA Polymerase | Takara Bio Inc | R045A | |
Commercial assay or kit | In-Fusion HD Cloning Kit | Clontech | 639,649 | |
Commercial assay or kit | DakoReal EnVision Detection System | Dako | K500711-2 | |
Commercial assay or kit | Peroxidase-Blocking Solution | Dako | S202386-2 | |
Commercial assay or kit | G-Block | GenoStaff | GB-01 | |
Commercial assay or kit | Blocking One Histo | nacalai tesque | 06349–64 | |
Commercial assay or kit | rat tail type I collagen | Corning Inc | 354,236 | |
Commercial assay or kit | DQ-collagen type I | Invitrogen | D12060 | |
Commercial assay or kit | 3D microfluidic cell culture chip | AIM Biotech | DAX-1 | |
Commercial assay or kit | QCM Gelatin Invadopodia Assay (Red) | Merck Millipore | ECM671 | |
Commercial assay or kit | poly-D-lysine | Sigma-Aldrich | P6407 | |
Commercial assay or kit | Matrigel Growth Factor Reduced | Corning Inc | 354,230 | |
Commercial assay or kit | 6.5 mm Transwell with 8.0 µm Pore Polycarbonate Membrane Insert | Corning Inc | 3,422 | |
Commercial assay or kit | BioCoat Matrigel Invasion Chambers with 8.0 µm PET Membrane | Corning Inc | 354,480 | |
Commercial assay or kit | Annexin V-FITC Apoptosis Detection Kit | nacalai tesque | 15342–54 | |
Commercial assay or kit | protein A Sepharose beads | GE Healthcare | 17078001 | |
Commercial assay or kit | Dynabeads Protein G | Thermo Fisher Scientific | DB10003 | |
Commercial assay or kit | Pierce Silver Stain for Mass Spectrometry | Thermo Fisher Scientific | 24,600 | |
Commercial assay or kit | O.C.T. Compound | Sakura Finetek | | |
Commercial assay or kit | NucleoSpin RNA | MACHEREY-NAGEL GmbH & Co. KG | 740,955 | |
Commercial assay or kit | ReverTra Ace qPCR RT Master Mix | TOYOBO | FSQ-201 | |
Commercial assay or kit | Pierce BCA Protein Assay Kit | Thermo Fisher Scientific | 23,227 | |
Chemical compound, drug | PD184161 | Sigma-Aldrich | PZ0112 | |
Chemical compound, drug | U0126 | Promega Corp. | V1121 | |
Chemical compound, drug | Rapamycin | Cell Signaling Technology | 9,904 | |
Chemical compound, drug | LY294002 | Cell Signaling Technology | 9,901 | |
Chemical compound, drug | VivoGlo luciferin | Promega Corp. | P1043 | |
Software, algorithm | HALO | Indica Labs | | RRID:SCR_018350 |
Software, algorithm | NanoZoomer-SQ | Hamamatsu Photonics K.K. | | |
Software, algorithm | UCSC Xena browser | http://xena.ucsc.edu | | RRID:SCR_018938 |
Software, algorithm | Kaplan–Meier plotter | http://www.kmplot.com | | RID:SCR_018753 |
Software, algorithm | GraphPad Prism 8 | GraphPad Software. | | RRID:SCR_002798 |
Software, algorithm | Excel Toukei | ESUMI Co., Ltd. | | |
Software, algorithm | Imaris | Bitplane | | RRID:SCR_007370 |
Software, algorithm | Image J | National Institutes of Health | | RRID:SCR_003070 |
Software, algorithm | Living Image 4.3.1 Software | Caliper Life Sciences | | RRID:SCR_014247 |
Software, algorithm | ikra v1.2.2 | https://zenodo.org/record/3606888 (Yu et al., 2019) | | |
Software, algorithm | iDEP.90 | http://bioinformatics.sdstate.edu/idep90/ (Ge et al., 2020) | | |
Software, algorithm | Ingenuity Pathway Analysis | IPA; Qiagen | | RRID:SCR_008653 |
Other | LSM880 laser scanning microscope | Carl Zeiss | | |
Other | BZ-9000 | Keyence | | |
Other | IVIS imaging system | Xenogen Corp. | | |
Other | Hoechst33342 | Invitrogen | H1399 | |
Other | Alexa Fluor 488 Phalloidin | Invitrogen | A12379 | |
Other | Alexa Fluor 546 Phalloidin | Invitrogen | A22283 | |
Other | Alexa Fluor 647 Phalloidin | Invitrogen | A22287 | |