(A) Growth curves of MDA-MB231 positioned inside the PTV and irradiated or not during 3 weeks. (B) Growth curves of NHDFs (donor F6MC1, population doubling [PD] = 30.36 at the beginning of the …
Data for growth curves of MDA-MB231.
Data for growth curves of normal human dermal fibroblasts (NHDFs).
Data for cell death assays of normal human dermal fibroblasts (NHDFs) and MDA-MB231.
(A) Image of the Varian Primus CLINAC used in the study (except experiments on mice). (B) Varian Primus CLINAC’s depth profile recorded in photon mode at 6 or 20 MV.
Data for the depth profile.
(A) Schematic representation of how 96-, 12-, or 6-well plates were irradiated. Plates were put on a 2- or 4-cm-thick poly(methyl methacrylate) (PMMA) plate laid on the carbon fiber table for an …
Growth curves of NHDFs (donor F1MC1, population doubling [PD] = 26.3 at the beginning of the experiment) cultured in 6-well plates irradiated at 2 Gy/day except weekends during 3 weeks. The days of …
Data for growth curves.
(A) MDA-MB231 cultured in 100 mm dishes were irradiated or not at 2 Gy/day except weekends up to six irradiations. The dishes were entirely located inside the PTV. NHDFs (A: donor F1MC1, population …
Data for Alexa Fluor 488 Annexin Dead Cell Apoptosis assays.
Data for Annexin V-positive cells.
Six-well plates of NHDFs (donor F1MC1, population doubling [PD] = 25.8 at the beginning of the experiment) positioned straddling the PTV and the margin were irradiated during 3 weeks at 2 Gy/day …
Data for SA-β-Gal activity.
Data for forward scatter (FSC) and side scatter (SSC levels).
NHDFs (donor F6MC1, population doubling [PD] = 32.4 at the beginning of the experiment) cultured in 12-well plates were irradiated or not at 2 Gy each day except weekends for a total of 10 radiation …
Values of C12FDG fluorescence intensity.
NHDFs (donor F1MC1, population doubling [PD] = 25.8 at the beginning of the experiment) cultured in 6-well plates were irradiated or not at 2 Gy/day except weekends up to 13 times. NHDFs at …
Quantification of cells in the different phases of the cell cycle.
NHDFs (donor F6MC1, population doubling [PD] = 18 at the beginning of the experiment) cultured in 12-well plates were irradiated or not at 2 Gy/day except weekends for different durations (2 × 2 Gy, …
RT-qPCR data for p16.
RT-qPCR data for p21.
NHDFs (donor F6MC1, population doubling [PD] = 18 at the beginning of the experiment) cultured in 12-well plates were irradiated or not at 2 Gy/day except weekends for different durations (1 × 2 Gy, …
Western blot of p16.
Quantification of p16-positive cells.
Three independent series of fluorescent microscopy images from three immunofluorescence experiments were reanalyzed for the presence of multinucleated cells. In these experiments, NHDFs were …
Quantification of multinucleated cells.
(A) Proliferating NHDFs (donor F1MC1, population doubling [PD] = 32) positioned straddling the PTV and the margin were irradiated once at 2 Gy. Cells were fixed 20 min or 2 hr later for the …
Number of XRCC1 foci.
Number of 53BP1 foci.
Statistics on comet assays at pH 12.3.
Comet assays at pH 12.3.
Comet assays at pH 8.
Statistics on comet assays at pH 8.
Proliferating normal human dermal fibroblasts (NHDFs) (donor F1MC1, population doubling [PD] = 32) cultured in 96-well plates positioned straddling the planning target volume (PTV) and the margin …
Proliferating NHDFs (donor F6MC1) cultured in 6-well plates positioned straddling the PTV were irradiated once at 2 Gy. Cells were fixed 2 hr later for the immunodetection of γH2AX and 53BP1, …
Quantification of γH2AX foci.
Proliferating NHDFs (F26MC1, population doubling [PD] = 6.2; F1MC1, PD = 24.3) cultured in 12-well plates were irradiated at 2 Gy and then processed for comet assays as in Figure 3B. About 100 cells …
Comet assays at pH 12.3 with F26MC1 NHDFs.
Statistics on comet assays at pH 12.3 with F26MC1 NHDFs.
Comet assays at pH 8 with F26MC1 NHDFs.
Statistics on comet assays at pH 8 with F26MC1 NHDFs.
Comet assays at pH 12.3 with F1MC1 NHDFs.
Statistics on comet assays at pH 12.3 with F1MC1 NHDFs.
Comet assays at pH 8 with F1MC1 NHDFs.
Statistics on comet assays at pH 8 with F1MC1 NHDFs.
Proliferating normal human dermal fibroblasts (NHDFs) (donor F1MC1, population doubling [PD] = 39) cultured in 96-well plates were irradiated as in Figure 3 at 2 Gy by a photon beam of 6 or 20 MV. …
Number of XRCC1 and 53BP foci.
(A) Schematic representation of how the 12-well plates were aligned, sealed or not together with an ultrasound gel (250820, Asept Inmed), in order to have wells inside the PTV and wells in the …
Number of XRCC1 foci.
Statistics on XRCC1 foci number.
Number of 53BP1 foci.
Statistics on 53BP1 foci number.
NHDFs’ (F6MC1, population doubling [PD] = 27.8) proliferation was slowed down by starvation in 0.1% fetal bovine serum (FBS) instead of the normal 2% FBS concentration. (A) Cell cycle analysis …
Quantification of cells in the different phases of the cell cycle.
Comet assays.
(A) Schematic representation of how mice were positioned at the margin of a phantom. (B) Six mice (three females and three males) were irradiated as in (A), one mouse was irradiated positioned …
Number of XRCC1 foci.
Statistics on XRCC1 foci numbers.
Number of 53BP1 foci.
Statistics on 53BP1 foci numbers.
(A) Mice irradiated as in Figure 4 were sacrificed 1 hr after irradiation. Skin samples at different distances from the isodose 50 (the spine), established as the zero, were dissected and …
Quantification of XRCC1 foci.
Statistics on the quantification of XRCC1 foci.
Proliferating NHDFs (donor F6MC1, population doubling [PD] = 22.67) cultured in 12-well plates were irradiated or not only one time at 2 Gy or during 5 days at 2 Gy daily. Just after the last …
Comet assays at pH 8 for the planning target volume (PTV).
Statistics on comet assays at pH 8.
Statistics on comet assays at pH 12.3.
Comet assays at pH 8 for the margin –5 + 20.
Comet assays at pH 8 for the margin +22 + 47.
Comet assays at pH 12.3 for the planning target volume (PTV).
Comet assays at pH 12.3 for the margin –5 + 20.
Comet assays for the margin +22 + 47.
Proliferating NHDFs (F6MC1, population doubling [PD] = 20.3 at the beginning of the experiment) cultured in 12-well plates were irradiated at 2 Gy/day for 1 (1 × 2 Gy) or 5 days (5 × 2 Gy). After …
Comet assays pH 12.3.
Statistics on comet assays pH 12.3.
Comet assays pH 8.
Statitstics on comet assays pH 8.
Proliferating NHDFs (F6MC1, population doubling [PD] = 19) cultured in 96-well plates were irradiated at 2 Gy/day for 4 days (4 × 2 Gy). Then, they were fixed at indicated time and an …
Quantification of XRCC1 foci.
Quantification of 53BP1 foci.
(A) Analysis of the PARylation capacity in normal human dermal fibroblasts (NHDFs) (F6MC1, population doubling [PD] = 32.4 at the beginning of the experiment) positioned at the margin or inside a …
Quantification of PAR foci.
Statistics on quantification of PAR foci.
(A) Normal human dermal fibroblasts (NHDFs) (donor F6MC1, population doubling [PD] = 29.6 at the beginning of the experiment) positioned straddling the planning target volume (PTV) and the margin …
Data of growth curves.
Quantification of 6-thioguanine (6-TG)-resistant cells.
Zymography.
Quantification of cell migration and invasion.
(A) NHDFs (donor F1MC1, population doubling [PD] = 26.83 at the beginning of the experiment) cultured in 6-well plates were irradiated or not at 2 Gy/day except weekends during 2 weeks. Cells were …
Growth curves.
Quantification of 6-thioguanine (6-TG)-resistant cells.
(A) Analysis of XRCC1 foci by immunofluorescence in normal human dermal fibroblasts (NHDFs) (F1MC1, population doubling [PD] = 44.07 at the beginning of the experiment) treated daily or not with …
Number of XRCC1 foci.
Quantification of SA-β-Gal assays.
Growth curves.
Quantification of 6-thioguanine (6-TG)-resistant cells.
(A) PARylation synthesis assay in normal human dermal fibroblasts (NHDFs) (F1MC1, population doubling [PD] = 46) treated during 24 hr with different concentrations of veliparib and challenged for 10 …
Western-blots.
Quantification of 53BP1 foci.
Quantification of hOGG1 foci.
Quantification of red on green fluorescence.
Normal human dermal fibroblasts (NHDFs) (F1MC1, population doubling [PD] = 44.07 at the beginning of the experiment) were treated daily or not with veliparib + H2O2 as in Figure 7. phosphoXRCC1 (A) …
Quantification of phosphoXRCC1 foci.
Quantification of polynucleotide kinase phosphatase (PNKP) foci.
NHDFs (F6MC1, population doubling [PD] = 40.04 at the beginning of the experiment) were submitted daily to the combined veliparib +H2O2 treatment as in Figure 6 for 31 days. Then, the H2O2 treatment …
Growth curves.
Quantification of SA-β-Gal assays.
Normal human dermal fibroblasts (NHDFs) (F1MC1, population doubling [PD] = 40.01 at the beginning of the experiment) were treated daily or not with veliparib + H2O2 as in Figure 6. (A) At day 25, …
Values of C12FDG fluorescence intensity.
Western blots.
Results of Annexin V/propidium iodide assays.
Non-irradiated | PTV | Margin (-5 to +20mm) | Margin (+22 to +47mm) | |
---|---|---|---|---|
Mean | 2.34 | 7.49 | 2.96 | 3.45 |
Fold change between PTV and margin | 2.53 | 2.17 |