IGF-1 facilitates extinction of conditioned fear
- Departamento de Anatomía, Histología y Neurociencia, Facultad de Medicina, Universidad Autónoma de Madrid, Spain
- Departamento de Ciencias Médicas Básicas (Fisiología) and Instituto de Tecnologías Biomédicas (ITB), Universidad de La Laguna, Spain
- Department of Neuroscience, University of Minnesota, United States
- Centro de Investigación Biomédica en Red Sobre Enfermedades Neurodegenerativas (CIBERNED), Instituto Universitario de Investigación Neuroquímica (IUIN), Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS) and Departamento de Bioquímica y Biología Molecular, Facultad de Química, Universidad Complutense de Madrid, Spain
- Institute of Physiology and Pathophysiology, Medical Biophysic, Heidelberg University, Germany
Figures
Figure 1 with 2 supplements
IGF-1 increases the excitability of IL-L5PNs.
(A) Representative recordings from IL-L5PNs of hyperpolarizing potentials elicited by an 800 ms depolarizing pulse to study medium and slow AHP in control conditions (ACSF, top) and in the presence …
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Figure 1—source data 1
Source data for Figure 1: IGF-1 increases the excitability of IL-L5PNs.
- https://cdn.elifesciences.org/articles/67267/elife-67267-fig1-data1-v2.xlsx
Figure 1—figure supplement 1
IGF-1 has no effect on fAHP and fIAHP.
(A) Representative recordings of hyperpolarizing potentials elicited by a 10 ms depolarizing pulse applied to study the fast components of the AHP, in control conditions (ACSF, left) and in the …
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Figure 1—figure supplement 1—source data 1
Source data for Figure 1—figure supplement 1.
IGF-1 has no effect on fAHP and fIAHP.
- https://cdn.elifesciences.org/articles/67267/elife-67267-fig1-figsupp1-data1-v2.xlsx
Figure 1—figure supplement 2
Action potential (AP) properties are unaltered by IGF-1.
(A–G) AP amplitude; threshold, half-width, time to rise slope, rise slope, time to decay slope, and decay slope in ACSF and during IGF-1 (n = 9 cells/7 animals) ACSF vs IGF-1 ns Student's paired …
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Figure 1—figure supplement 2—source data 1
Source data for Figure 1—figure supplement 1: Action potential properties are unaltered by IGF-1.
- https://cdn.elifesciences.org/articles/67267/elife-67267-fig1-figsupp2-data1-v2.xlsx
Figure 2 with 1 supplement
IGF-1 reduces mIAHP and sIAHP in IL-L5PNs.
(A) Representative current traces recorded from IL-L5PNs in response to an 800 ms depolarizing voltage pulse from −60 mV to 0 mV in the control condition (ACSF, top) and the presence of NVP-AEW541 …
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Figure 2—source data 1
Source data for Figure 2.
IGF-1 reduces mIAHP and sIAHP in IL-L5PNs.
- https://cdn.elifesciences.org/articles/67267/elife-67267-fig2-data1-v2.xlsx
Figure 2—figure supplement 1
Time course of mIAHP and sIAHP.
(A) Plot showing the time course of mIAHP (left) and sIAHP (right) in control conditions (ACSF) after applying 800 ms depolarizing pulses, respectively (n = 4 in each group). Note that the IAHPs are …
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Figure 2—figure supplement 1—source data 1
Source data for Figure 2—figure supplement 1.
Time course of mIAHP and sIAHP.
- https://cdn.elifesciences.org/articles/67267/elife-67267-fig2-figsupp1-data1-v2.xlsx
Figure 3 with 2 supplements
IGF-1 induces long-term potentiation of the synaptic transmission.
(A) Plot showing the time course of EPSCs when the IGF-1 was bath applied for 35 min after a stable (~5 min) baseline, (n = 5 cells/3 animals, ACSF vs IGF-1 ***p<0.001 and ns, n = 5 cells/3 animals …
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Figure 3—source data 1
Source data for Figure 3.
IGF-1 induces long-term potentiation of the synaptic transmission.
- https://cdn.elifesciences.org/articles/67267/elife-67267-fig3-data1-v2.xlsx
Figure 3—figure supplement 1
The protocol of stimulation does not induce plasticity.
(A) Plot showing a long-lasting stable recording of the PSPs with the protocol used in Figure 3I but in the absence of IGF-1 ACSF (−5–0 min) vs ACSF (45–50 min), ns (not-significant) Student's …
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Figure 3—figure supplement 1—source data 1
Source data for Figure 3—figure supplement 1.
The protocol of stimulation does not induce plasticity.
- https://cdn.elifesciences.org/articles/67267/elife-67267-fig3-figsupp1-data1-v2.xlsx
Figure 3—figure supplement 2
The application of IGF-1 is sufficient to induce plasticity.
(A) Plot showing the time course of PSPs amplitude when, after a stable (~5 min) baseline, IGF-1 was bath applied for 10 min. Student's paired t-test ***p>0.001 (n = 6 cells/2 animals). (B) Bar …
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Figure 3—figure supplement 2—source data 1
Source data for Figure 3—figure supplement 2.
The application of IGF-1 is sufficient to induce plasticity.
- https://cdn.elifesciences.org/articles/67267/elife-67267-fig3-figsupp2-data1-v2.xlsx
Figure 4 with 2 supplements
IGF-1 facilitates the extinction memory by reducing mIAHP and sIAHP.
(A) (top) Image showing the infralimbic cortex (IL) localization. (bottom) Nissl-stained coronal section of rat IL, the orange arrow indicates the tip of the cannula implanted on the IL. (B) Plot …
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Figure 4—source data 1
Source data for Figure 4.
IGF-1 facilitates the extinction memory by reducing mIAHP and sIAHP.
- https://cdn.elifesciences.org/articles/67267/elife-67267-fig4-data1-v2.xlsx
Figure 4—figure supplement 1
Protocol of behavior.
(A) The experimental design of the conditioned fear experiments. (B) Plot showing the time course of the percentage of freezing during the experiment for the two groups studied: conditioned (COND, …
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Figure 4—figure supplement 1—source data 1
Source data for Figure 4—figure supplement 1.
Protocol of behavior.
- https://cdn.elifesciences.org/articles/67267/elife-67267-fig4-figsupp1-data1-v2.xlsx
Figure 4—figure supplement 2
AHPs of the L5PN of the IL recorded from animals after fear extinction.
(A) Bar diagram summarizing fAHP from rats NAÏVE (n = 10 cells/8 animals) and after test behaviors in different groups: EXT (n = 6 cells/2 animals); Ext-Saline (n = 10 cells/3 animals); Ext-IGF-1 (n …
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Figure 4—figure supplement 2—source data 1
Source data for Figure 4—figure supplement 2.
AHPs of the L5PN of the IL recorded from animals after fear extinction.
- https://cdn.elifesciences.org/articles/67267/elife-67267-fig4-figsupp2-data1-v2.xlsx
Figure 5
IGF-1 decreases the frequency of mEPSC.
(A) Representative traces recorded at −60 mV in IL-L5PNs from animals that showed fear extinction, in the presence of 1 µM TTX, 50 µM PiTX, and 5 μM CPG-55845. Asterisks denote mEPSC events. Note …
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Figure 5—source data 1
Source data for Figure 5: IGF-1 decreases the frequency of mEPSC.
- https://cdn.elifesciences.org/articles/67267/elife-67267-fig5-data1-v2.xlsx
Additional files
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Supplementary file 1
Summary of statistical significance and tests used in each figure.
- https://cdn.elifesciences.org/articles/67267/elife-67267-supp1-v2.docx
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Transparent reporting form
- https://cdn.elifesciences.org/articles/67267/elife-67267-transrepform-v2.docx
