Effects of arousal and movement on secondary somatosensory and visual thalamus
- Department of Neuroscience, Columbia University, United States
- Kavli Institute for Brain Science, United States
- Zuckerman Mind Brain Behavior Institute, United States
Figures
Figure 1 with 2 supplements
POm cells mainly track slow components of whisking activity.
(A) An example frame from a video. Identified whiskers are highlighted in green. (B) Example traces of juxtasomal POm recording and whisking. The median angle of all whiskers in each video frame …
Figure 1—figure supplement 1
Anatomical confirmation of recording locations.
(A, B) POm juxtasomal recording locations labeled with DiI in two example wild-type mice. (C) POm juxtasomal recording location in an example Emx1-Halo mouse (Green: GFP; Orange: DiI). (D) VPM …
Figure 1—figure supplement 2
POm cells are coherent with whisking only at low frequencies.
(A) Individual (gray) and mean (red) power spectra of mouse whisking (n=11 mice). (B) Mean coherence between POm spiking activity and whisking (green) compared to shuffled data (black). Shaded: SEM …
Figure 2
POm encodes whisking activity in absence of reafferent sensory input.
(A) The buccal and upper marginal branches of the facial motor nerve were severed unilaterally, preventing whisker motion on the right side of the face. Adapted from Heaton et al., 2014. (B) Motion …
Figure 3
Inhibition of primary motor cortex increases POm correlation with whisking.
(A) Experimental setup. M1 was optogenetically silenced while recordings were made from M1, POm, or VPM. Adapted from The Mouse Brain Atlas in Stereotaxic Coordinates (Paxinos and Franklin, 2019). (B…
Figure 4
Inhibition of primary somatosensory cortex increases POm correlation with whisking.
(A) Experimental setup. S1 was optogenetically silenced while recordings were made from POm or VPM. The Mouse Brain Atlas in Stereotaxic Coordinates (Paxinos and Franklin, 2019). (B) Individual …
Figure 5
Lesions to superior colliculus (SC) do not reduce POm correlation with whisking.
(A) Sample coronal section showing bilateral electrolytic lesion of SC. (B) Scatter plot of POm firing rates during whisking and quiescence in lesioned (red) and intact animals (black, data from Figu…
Figure 6
POm activity tracks pupil dynamics.
(A) Sample recording of POm activity (middle, black) with concurrent ipsilateral pupil diameter (blue, top), median whisker angle (middle, gray), and whisking amplitude (green, bottom). (B) …
Figure 7 with 1 supplement
LP activity tracks slow whisker dynamics.
(A, B) Sample recordings of two LP cells (black) recorded in normal light (A) or low light (B), with corresponding median whisker angle (gray) whisking amplitude (green), and pupil diameter (blue or …
Figure 7—figure supplement 1
POm and LP cells have identical patterns of coherence with whisking.
(A) Mean spiking-whisking coherence for each POm and LP cell in the frequency ranges 0–5 Hz and 8–13 Hz. Green circles: POm (n=22 cells from five mice, same as in Figure 1). Blue diamonds: POm with …
