Mice exposed to JUUL Mint aerosols three times daily (60 min total) for 1 month prior to LPS challenge had increased expression of Tnfa, Il1b, Ccl2, Ccl3, Cxcl1, and Cxcl2, and decreased Postn, relative to air controls, while mice exposed to JUUL Mango aerosols for 1 month had increased Ccl2 and decreased Postn. After 3 months of JUUL Mint exposure, cardiac tissue had increased Il1b, Ccl2, Il1b, Ccl2, and Col3a1 in the setting of LPS challenge, while JUUL Mango had increased Il1b. Data were analyzed with one-way ANOVA with Holm-Sidak multiple comparisons for each gene of interest. Data are presented as individual data points ± SD with n = 5–6 mice per group. *p < 0.05, **p < 0.01, *** p < 0.001, and ****p < 0.0001.