Lipoprotein DolP supports proper folding of BamA in the bacterial outer membrane, promoting fitness upon envelope stress
- CNRS, France
- Institut Pasteur, France
Abstract
Integral outer membrane proteins (OMPs) are crucial for the maintenance of the proteobacterial envelope permeability barrier to some antibiotics and detergents. In Enterobacteria, envelope stress caused by unfolded OM proteins (OMPs) activates the sigmaE (σE) transcriptional response. σE upregulates OMP-biogenesis factors, including the b-barrel assembly machinery (BAM) that catalyzes OMP folding. Here we report that DolP (formerly YraP), a σE-upregulated and poorly understood OM lipoprotein, is crucial for fitness in cells that undergo envelope stress. We demonstrate that DolP interacts with the BAM complex by associating to OM-assembled BamA. We provide evidence that DolP is important for proper folding of BamA that overaccumulates in the OM, thus supporting OMP biogenesis and OM integrity. Notably, mid-cell recruitment of DolP had been linked to regulation of septal peptidoglycan remodelling by an unknown mechanism. We now reveal that, during envelope stress, DolP loses its association with the mid-cell, thereby suggesting a mechanistic link between envelope stress caused by impaired OMP biogenesis and the regulation of a late step of cell division.
Data availability
All data generated and analysed during this study are available in the manuscript and supporting files. Source data related to the CRISPRi screen are provided in the supporting files.
Article and author information
Author details
Luis Orenday-Tapia
Funding
Centre National de la Recherche Scientifique (ATIP-Avenir)
- Raffaele Ieva
Agence Nationale de la Recherche (ANR-10-INBS-08)
- Julien Marcoux
Agence Nationale de la Recherche (ANR-10-LABX-62-IBEID)
- David Bikard
Fondation pour la Recherche Médicale (PostDoc Fellowship)
- David Ranava
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Bavesh D Kana, University of the Witwatersrand, South Africa
Version history
- Received: February 24, 2021
- Accepted: April 4, 2021
- Accepted Manuscript published: April 13, 2021 (version 1)
- Version of Record published: April 28, 2021 (version 2)
Copyright
© 2021, Ranava et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Lipoprotein DolP supports proper folding of BamA in the bacterial outer membrane, promoting fitness upon envelope stress
eLife 10:e67817.
https://doi.org/10.7554/eLife.67817
Further reading
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