(A) Experimental design. (B) Plasma cholesterol. Aortic root morphometric analysis for areas of (C) plaque, (D) CD68, and (E) %of plaque positive for CD68. (F) Representative images and (G) quantification of aortic roots stained for (from left to right) CD68, collagen (with picrosirius red, imaged in bright field and under polarized light), vascular cell adhesion molecule 1, C-C motif chemokine ligand 2, mannose receptor 1MRC1, and arginase 1. Scale bar, 200 µm for CD68 and collagen, 50 µm for others. The dashed line demarcates plaque, and L stands for lumen. ****p<0.0001, ***p<0.001, **p<0.01, and *p<0.05 determined via one-way ANOVA comparing each group to the other groups, with Dunnett’s multiple comparison test.