(A) Coronal sections of the R. pumilio SCN taken across the rostro-caudal axis labeled with DAPI, and immunofluorescence for the main SCN neuropeptides: (B) Arginine-vasopressin (AVP), (C) …
(A) Whole-cell patch clamp recording setup showing bright-field image of a SCN coronal brain slice. The SCN (delineated by white dotted lines) can be observed above the optic chiasm (OC), on either …
Numerical data to support graphs in Figure 2C–F.
Representative current-clamp traces showing the different type of responses to a depolarizing pulse (1 s, +30 pA): (A) non-adapting; (B) adapting-firing; or (C) adapting-to-silent response. …
Numerical data to support graphs in Figure 3D and G.
(A) Schematic of conductance-based model for R. pumilio SCN neurons containing sodium (INa), calcium (ICa), potassium (IK), and leak (ILNa, ILK) currents. Orange resistors (gNa, gCa, gK) indicate …
(A–F) Current-clamp recordings (blue) with the portion of the voltage trace used by the data assimilation algorithm (orange) to fit the model shown in Figure 4 and Figure 4—figure supplement 2. (A–C)…
(A–F) Current-clamp recordings (black) and simulated voltage traces (red) from the model shown in Figure 4 that was fit using the portions of the data shown in Figure 4—figure supplement 1. (A–C) …
(A) Voltage trace showing spontaneous firing in model of R. pumilio SCN neurons. (a1) Magnified view of second AP shown in (A). (B) Sodium (INa, blue), calcium (ICa, magenta), potassium (IK, green), …
(A) Voltage at steady-states and maximum/minimum voltage of oscillations for the model of rebound spiking in the Type-A neuron shown in Figures 4 and 6A, and Figure 4—figure supplements 1–3 with …
(A–B) Voltage traces of models (red) and current-clamp recordings (black) during depolarizing pulses (1 s, +30 pA) showing non-adapting (i), adapting-firing (ii), and adapting-to-silent (iii) …
(A–B) Voltage traces of models (red) and current-clamp recordings (black) during hyperpolarizing pulses (1s, -30 pA) showing rebound spiking of Type-A neurons (A) and delay responses of Type-B cells …
Numerical data to support graphs in Figure 6I.
(A) Heatmap showing the overall effects of inhibitory (gsyn-I, red) and excitatory (gsyn-E, blue) physiological GABAergic synaptic conductances on firing frequency with increasing IA conductances in …
Representative trace from a SCN neuron (voltage-clamped at −70 mV) showing post-synaptic currents (PSCs) under baseline conditions (top). Bath application of the GABAA receptor blocker, Bicuculline …
Parameter values for the computational models of Rhabdomys pumilio SCN neurons.
Models were fit to data from seven different neurons, including a non-adapting cell (Figure 5Ai), an adapting-firing cell (Figure 5Aii), an adapting-silent cell (Figure 5Aiii), two Type-A rebound spiking cells (Figures 4 and 6A), and two Type-B delay cells (Figure 6B and O). These cells were chosen for modeling because they are representative of the various responses observed across all the recordings. Each model was fit to data from six voltages traces as illustrated in Figure 4—figure supplement 1.