PBN-PVT projections modulate negative affective states in mice
- Shanghai Jiao Tong University School of Medicine, China
- Tongji University School of Medicine, China
- The First Affiliated Hospital of Guangzhou Medical University, China
Abstract
Long-lasting negative affections dampen enthusiasm for life, and dealing with negative affective states is essential for individual survival. The parabrachial nucleus (PBN) and thalamic paraventricular nucleus (PVT) are critical for modulating affective states in mice. However, the functional roles of PBN-PVT projections in modulating affective states remain elusive. Here, we show that PBN neurons send dense projection fibers to the PVT and form direct excitatory synapses with PVT neurons. Activation of the PBN-PVT pathway induces robust behaviors associated with negative affective states without affecting nociceptive behaviors. Inhibition of the PBN-PVT pathway reduces aversion-like and fear-like behaviors. Furthermore, the PVT neurons innervated by the PBN are activated by aversive stimulation, and activation of PBN-PVT projections enhances the neuronal activity of PVT neurons in response to the aversive stimulus. Consistently, activation of PVT neurons that received PBN-PVT projections induces anxiety-like behaviors. Thus, our study indicates that PBN-PVT projections modulate negative affective states in mice.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting file. The behavioral data and imaging analysis results have been made available on Dryad Digital Repository (https://doi:10.5061/dryad.1rn8pk0w4). All MATLAB code has been deposited at: https://github.com/laizishangalali/Xiang/blob/main/zscore_KS_test.m and is publicly available.
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PBN-PVT projections modulate negative affective states in miceDryad Digital Repository, doi:10.5061/dryad.1rn8pk0w4.
Article and author information
Author details
Funding
National Natural Science Foundation of China (31900717)
- Di Mu
China Association for Science and Technology (2019QNRC001)
- Di Mu
Shanghai Association for Science and Technology (19YF1438700)
- Di Mu
National Natural Science Foundation of China (31571086)
- Ling Zhang
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: All animal experiment procedures were approved by the Animal Care and Use Committee of Shanghai General Hospital (2019AW008).
Copyright
© 2022, Zhu et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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PBN-PVT projections modulate negative affective states in mice
eLife 11:e68372.
https://doi.org/10.7554/eLife.68372
Further reading
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Methods:
In this study, we investigated the impact of severe AUD (sAUD), and of a 3-week period of alcohol abstinence, on the blood metabolome (non-targeted LC-MS metabolomics analysis) in 96 sAUD patients hospitalized for alcohol withdrawal.
Results:
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Conclusions:
Our data allow the identification of neuroactive metabolites, from interactions between food components and microbiota, which may represent new targets arising in the management of neuropsychiatric diseases such as sAUD.
Funding:
Gut2Behave project was initiated from ERA-NET NEURON network (Joint Transnational Call 2019) and was financed by Academy of Finland, French National Research Agency (ANR-19-NEUR-0003-03) and the Fonds de la Recherche Scientifique (FRS-FNRS; PINT-MULTI R.8013.19, Belgium). Metabolomics analysis of the TSDS samples was supported by grant from the Finnish Foundation for Alcohol Studies.
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