(A) Defining meta-clonotypes from antigen-associated TCRs. Sets of antigen-associated TCRs were used together with synthetic background repertoires to engineer TCR meta-clonotypes that define …
(A) Correspondence between edit distance (x-axis) and TCRdist (y-axis) for MIRA55 T-cell receptors (TCRs) with matching TRBV genes. The grayscale colormap shows the percentage of TCRs with a given …
(A) TCR repertoire subsets obtained by single-cell sorting with peptide–major histocompatibility complex (MHC) tetramers (green), MIRA peptide stimulation enrichment (MIRA55, MIRA48; purple), or …
TCR β-chains from (A) a peptide–major histocompatibility complex (MHC) tetramer-enriched subrepertoire (n = 247), (B) a MIRA peptide stimulation-enriched subrepertoire (n = 497), or (C) an umbilical …
(A) Each T-cell receptor (TCR) (one line, n = 497) in the MIRA55 antigen-associated set acts as the centroid of a neighborhood and an empirical cumulative distribution function (ECDF) is estimated …
The grid shows all features that were present in two or more MIRA participants. TCR feature publicity across individuals was assessed using two methods: (1) tcrdist3 meta-clonotypes (rectangles) – …
(A) Radius estimates for MIRA55 T-cell receptors (TCRs) using different synthetic backgrounds: (i) randomly generated TCRs from Optimized Likelihood estimate of Immunoglobulin Amino acid sequences …
(A) Percentage of TCR features with a statistically significant (false discovery rate [FDR] <0.01) association with a restricting HLA allele. We tested for associations between patients’ inferred …
(A) Beta-binomial regression coefficient estimates (x-axis) and negative log10 false discovery rates (y-axis) for features developed from CD8+ TCRs activated by SARS-CoV-2 MIRA55 ORF1ab amino acids …
Logo plots of T-cell receptors (TCRs) from bulk repertoires of acute and convalescent COVID-19 patients (n = 694) within 20 TCRdist units of MIRA-identified TCR β-chain meta-clonotype M_55_1E6+ …
TCR feature publicity was determined using two methods for clustering similar TCR sequences: (A) tcrdist3-identified meta-clonotypes and (B) GLIPH2 specificity groups, sets of TCRs with a shared …
(A) Beta-binomial regression coefficient estimates (x-axis) for participant genotype matching a hypothesized restricting HLA allele and negative log10 false discovery rates (FDRs; y-axis) for …
We evaluated 1831 meta-clonotypes from 17 MIRA sets in a cohort of 694 COVID-19 patients for their association with predicted HLA-restricting alleles. Statistical evidence of the HLA association for …
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Software, algorithm | Python3,Numpy,Pandas, | Python Programming Language, RRID:SCR_008394NumPy, RRID:SCR_00863Pandas, RRID:SCR_01821 | ||
Software, algorithm | R, ggplot2 | R Project for Statistical Computing, RRID:SCR_001905ggplot2, RRID:SCR_014601 | ||
Software, algorithm | tcrdist3 | This study | tcrdist3 0.2.0 | https://github.com/kmayerb/tcrdist3 |
Software, algorithm | pwseqdist | This study | pwseqdist 0.5 | https://github.com/agartland/pwseqdist |
Script, algorithm | hla3 | This study | version 0.1.0 | https://github.com/kmayerb/hla3 |
Software, algorithm | corncob | Martin et al., 2020 doi:10.1214/19-aoas1283 | https://github.com/bryandmartin/corncob (Martin, 2021) | |
Software, algorithm | OLGA | Sethna et al., 201910.1093/bioinformatics/btz035 | https://github.com/statbiophys/OLGA(Isacchini, 2021) See slight modifications in: https://github.com/kmayerb/tcrdist3/blob/master/tcrdist/olga_directed.py | |
Software, algorithm | GLIPH2 | Huang et al., 202010.1038/s41587-020-0505-4 | version 2 | http://50.255.35.37:8,080 |
Supporting data and analysis results.