Super-plots of (A) protrusion rate, (B) protrusion duration, (C) retraction rate, and (D) retraction duration, measured from kymographs. Number of fields of view (indicated by the opaque data points in the above graphs, with the mean and 95% CI displayed) analyzed for each population: β-actin = 11, γ-actin = 14, β-coded γ-actin = 13, γ-coded β-actin = 14. Number of protrusions analyzed: β-actin = 139, γ-actin = 130, β-coded γ-actin = 143, γ-coded β-actin = 121. Number of retractions analyzed: β-actin = 112, γ-actin = 108, β-coded γ-actin = 124, γ-coded β-actin = 101. One-way non-parametric analysis of variance (ANOVA) (Kruskal-Wallis test) gave a p-value = 0.0798 (protrusion rate), 0.0540 (protrusion duration), 0.1065 (retraction rate), and 0.0002 (retraction duration) with multiple comparisons shown on the plot. *p<0.05, **p<0.01, and ***p<0.001.