Cohen’s d effect size in ACC Color selective responses in neurons of different e-types in LPFC and ACC. (A, B) For LPFC neurons the average normalized firing of each e-type (in color) when motion was feature-1 and color was feature-2 (A) and when color was feature-1 and motion was feature-2 (B). Thickened line segments denote significant modulation over pre-feature firing levels at p<0.05. (C) Difference of firing aligned to feature-1 (left) and feature-2 (right) shows that e-types N2 and N3 responded significantly stronger to color onsets than motion onsets irrespective of whether color was shown first or second. (D-F) Same as a-c for neurons and e-types in ACC. The only consistent effect was for e-type B2 neurons showing a transient onset response to the first feature irrespective of whether it was color or motion. See Supplementary file 1 for Cohen’s d effect size measures for each cell type to rule out that we overlooked significant effects because of low number of cells in a cell class. For example, in LPFC, the firing rates of the N2 and N3 e-type increased significantly to the color cue (p<0.05; effect size values for N2, N3 are −0.491,–0.300). (G, H) Reward-activated neurons of different e-types in LPFC and ACC. For LPFC, the average normalized firing of each e-type to the reward onset (G) show moderately increased firing rate in most e-types. B1 e-type neurons showed stronger activation compared with other e-types (p<0.05, randomization test). In ACC (H), the N2 e-type neurons showed stronger activation to the reward onset compared with other e-types (p<0.05, effect size values for B1, N2 are −0.311,–0.367 in LPFC and ACC, respectively).