Proportion of activated GCs (A–C) and magnitude of activation (D) in response to PP stimulation (early postnatal P14-21, columns 1 and 2; young adult P48-91, columns 3 and 4), with deconvolved data for the young adult time point (columns 5 and 6). Wild-type in blue; Scn1a+/- in red. Data were analyzed using a mixed model to account for potential variability between animal, slice, field, and cell. Proportion of responsive GCs was calculated (A) relative to all GCaMP-expressing cells, or restricting analysis (B) to only GCs that respond to PP stimulation in the presence of 100 µM picrotoxin or (C) to only GCs that respond to the maximal stimulation delivered. (D) Magnitude of activated GC responses to PP stimulation, expressed as natural log dF/F0 (columns 1–4) or estimated spikes based on deconvolution (columns 5–6; size of data points reflects number of cells at that value). Dots: raw data from all imaging fields or cells; dark lines: average of fits; shaded: 95% confidence intervals of average fits. Stars indicate significant differences in curve fits: *, p < 0.05; **, p < 0.01; ***, p < 0.001. For early postnatal mice, n for the experiments in rows A, C, and D was, for Scn1a+/- and WT, respectively: 991 and 1,210 (cells), 51 and 58 (fields), 31 and 29 (slices), 11 and 10 (mice); for row B: 372 and 445 (cells), 16 and 17 (both fields and slices), 5 and 5 (mice). For the young adult mice, n for the experiments in rows A, C, and D: 1236 and 1167 (cells), 17 and 17 (both fields and slices), 8 and 6 (mice); for row B: 1109 and 1073 (cells), 16 and 14 (both fields and slices), 6 and 7 (mice).