In vivo analysis reveals that ATP-hydrolysis couples remodeling to SWI/SNF release from chromatin
- Erasmus University Medical Center, Netherlands
- Erasmus MC, Netherlands
- ErasmusMC, Netherlands
Abstract
ATP-dependent chromatin remodelers control the accessibility of genomic DNA through nucleosome mobilization. However, the dynamics of genome exploration by remodelers, and the role of ATP hydrolysis in this process remain unclear. We used live-cell imaging of Drosophila polytene nuclei to monitor Brahma (BRM) remodeler interactions with its chromosomal targets. In parallel, we measured local chromatin condensation and its effect on BRM association. Surprisingly, only a small portion of BRM is bound to chromatin at any given time. BRM binds decondensed chromatin but is excluded from condensed chromatin, limiting its genomic search space. BRM-chromatin interactions are highly dynamic, whereas histone-exchange is limited and much slower. Intriguingly, loss of ATP hydrolysis enhanced chromatin retention and clustering of BRM, which was associated with reduced histone turnover. Thus, ATP hydrolysis couples nucleosome remodeling to remodeler release, driving a continuous transient probing of the genome.
Data availability
The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (www.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD025474.All data generated or analyzed during this study are included in the manuscript and supporting files. Source data files have been provided for Figures 1, 2 and Figure 2-figure-supplement1
Article and author information
Author details
Funding
FOM-AMOLF (DNA at Work)
- Peter Verrijzer
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Sebastian Deindl, Uppsala University, Sweden
Version history
- Received: April 14, 2021
- Preprint posted: April 23, 2021 (view preprint)
- Accepted: July 26, 2021
- Accepted Manuscript published: July 27, 2021 (version 1)
- Version of Record published: August 9, 2021 (version 2)
Copyright
© 2021, Tilly et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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In vivo analysis reveals that ATP-hydrolysis couples remodeling to SWI/SNF release from chromatin
eLife 10:e69424.
https://doi.org/10.7554/eLife.69424
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