(A) Domain structure of STX1A. The protein consists of a short N-peptide (aa 1–9 or 1–28), Habc domain (aa 29–144) formed by three helices, Ha, Hb, and Hc, followed by the H3 helix (aa 189–259; …
Quantification of the STX1AWT and mutant STX1A expression induced by lentiviral transduction of STX1-null neurons and the consequent neurotransmitter release properties.
(A) Example images (left) and quantification of immunofluorescence labeling for Bassoon and STX1A shown as red and green, respectively, in the corresponding composite pseudocolored images obtained …
Quantification of the expression of FLAG-tagged WT and mutant STX1A.
(A) Example images of high-density cultures of STX1-null, STX1AWT, and STX1A∆Habc hippocampal neurons at DIV 8, 15, 22, and 29 represented with immunofluorescent labeling of microtubule associated …
Quantification of the neuronal density at different time intervals and quantification of ultrastructural synaptic properties in high density cultures of STX1-null, STX1AWT, and STX1AΔΗabc neurons.
(A) Example images of immunofluorescence labeling for Bassoon, STX1A, and Munc18-1 shown as red, green, and blue, respectively, in the corresponding composite pseudocolored images obtained from …
Quantification of the lentiviral expression of STX1AWT and STX1AΔΝ mutants in STX1-null neurons and the consequent neurotransmitter release properties.
(A) Example images of immunofluorescence labeling for Bassoon, STX1A, and Munc18-1 shown as red, green, and blue, respectively, in the corresponding composite pseudocolored images obtained from …
Quantification of lentiviral expression of phosphonull and phosphomimetic STX1A mutants and the consequent neurotransmitter release properties.
(A) Example images of high-density cultures of STX1-null hippocampal neurons at DIV 8 and 29 represented with immunofluorescent labeling of microtubule associated protein 2 (MAP2). Red and green …
Quantification of neuronal density of STX1AΔΝ and phosphorylation mutant neurons in comparison to STX1AWT and STX1-null neurons at DIV 8 and DIV 29.
(A) Example images of immunofluorescence labeling for Bassoon, STX1A, and Munc18-1 shown as red, green, and blue, respectively, in the corresponding composite pseudocolored images obtained from …
Quantification of lentiviral expression of STX1ALEOpen and STX1ALEOpen + ΔΝ mutants in STX1-null neurons and the consequent neurotransmitter release properties.
(A) Example images of high-density cultures of STX1-null hippocampal neurons at DIV 8, 15, 22, 29, 36, and 43 represented with immunofluorescent labeling of microtubule associated protein 2 (MAP2). …
Quantification of neuronal density of neurons expressing STX1ALEOpen or STX1ALEOpen + ΔΝ mutants at different time intervals.
(A) Example images of immunofluorescence labeling for VGlut1, STX1A, Munc18-1, and NLS-GFP shown as red, green, blue, and gray, respectively, in autaptic STX1-null hippocampal neurons rescued with …
Quantification of the effects lentiviral downtitration of STX1AWT and STX1ALEOpen on STX1A's and Munc18-1's expression levels and on neurotransmitter release parameters.
(A) Example images of immunofluorescence labeling for VGlut1, STX1A, and Munc18-1. Scale bar: 10 µm. (B) Quantification of the immunofluorescence intensity of STX1A as normalized to the …
Comparison of the lentiviral expression of STX1A with its endogenous expression.
(A) Example high-pressure freezing fixation combined with electron microscopy (HPF-EM) images of nerve terminals from high-density cultures of STX1AWT, STX1A∆N2-28, STX1ALEOpen, and STX1ALEOpen + …
Quantification of the ultrastructural synaptic properties of STX1AWT, STX1AΔΝ2-28, STX1ALEOpen, and STX1ALEOpen + ΔΝ2-28 neurons.
(A) Quantification of VGlut1-positive puncta using the images exemplified in Figure 4—figure supplement 2 and obtained from STX1AWT, STX1ALEOpen, and STX1ALEOpen + ∆N2-28 neurons. (B) Quantification …
Quantification of the number the total area of VGlut1 positive puncta in autaptic neurons expressing STX1AWT, STX1ALEOpen, or STX1ALEOpen + ΔΝ2-28.
(A) Example traces (left) and quantification (right) of STP measured by 50 stimulations at 10 Hz from STX1AWT, STX1A∆N2-9, STX1A∆N2-19, or STX1A∆N2-28 neurons. The traces show the absolute values, …
Quantification of the STP, recovery of RRP, RRP fraction released by 250 mM sucrose solution application and Ca2+-sensitivity of the vesicles in neurons expressing STX1AWT, STX1AΔΝ- or STX1ALEOpen mutants.
(A) Quantification of absolute values of 50 EPSCs elicited at 10 Hz recorded from neurons expressing either STX1AWT, STX1A∆N2-9, STX1A∆N2-19, or STX1A∆N2-28. (B) Example traces (left) and …
Quantification of the absolute values of EPSCs during STP and Ca2+-sensitivity of the vesicles in neurons expressing STX1AWT, STX1AΔΝ- or STX1ALEOpen mutants.
(A) Zoomed-in example traces of the first and last five EPSCs recorded by 50 stimulations at 10 Hz from STX1AWT, STX1A∆N2-9, STX1A∆N2-19, or STX1A∆N2-28 neurons. The traces show the absolute values. …
(A, B) Example images and average of SynGCaMP6f fluorescence as (ΔF/F0) in STX1-null neurons either not rescued or rescued with STX1AWT, STX1A∆N2-9, STX1A∆N2-19, STX1ALEOpen, or STX1ALEOpen + ∆N2-28.…
Quantification of the increase in SynGCaMP6f signal recorded at baseline or different numbers of APs in neurons expressing STX1AWT, STX1AΔΝ- or STX1ALEOpen mutants.
(A, B) Example images and average of SynGCaMP6f fluorescence as (ΔF/F0) in STX1-null neurons either not rescued or rescued with 1× or 1/12× viral volume for STX1AWT. The images were recorded at …
Quantification of the increase in SynGCaMP6f signal recorded at baseline or different numbers of APs in neurons expressing STX1AWT at low level.