(A) There were 82 overlapping genes between the day 3 (N=21) and week 2 (N=24) ADCP enriched genes in the RV306 study. (B) The model using GES obtained from the 82 genes was also able to predict ADCP responses measured 2 weeks after vaccination with an accuracy of 0.71. The ROC curve illustrates the discriminating ability of the classifier from the day 3 training data set (AUC=0.81, 95% CI: 0.62–1, p=0.007) and the week 2 testing data set (AUC=0.75, 95% CI: 0.53–0.97, p=0.02) to predict ADCP responses. (C) Heatmap showing the hierarchical clustering of gene expression of the 82 genes (day 3 and week 2 after 4th vaccination) when stratified by magnitude of ADCP responses measured 2 weeks after the 4th immunization. (D) The list of 82 ADCP enriched genes was uploaded in GeneMANIA. Edges represent physical interactions, co-expression, co-localization, and shared pathways. Circles depict the 82 genes, gold circles are the four genes that belong to the gene ontology Phagocytosis pathway, blue circles are genes that are directly connected to them, diamonds indicate related pathways, and the color of the edge indicates the type of connection. ADCP, antibody-dependent cellular phagocytosis; CI, confidence interval; GES, gene expression score; ROC, receiver operator characteristic.