Gene (Homo sapiens) | PCDH1 | GenBank | Gene ID: 5097 | |
Gene (Homo sapiens) | ITGB3 | GenBank | Gene ID: 3690 | |
Gene (Homo sapiens) | DAF | GenBank | Gene ID: 1604 | |
Gene (Homo sapiens) | ITGB1 | GenBank | Gene ID: 3688 | |
Strain, strain background (virus) | rVSV eGFP ANDV Gn/Gc | Kleinfelter et al., 2015 | | |
Strain, strain background (virus) | rVSV eGFP SNV Gn/Gc | Kleinfelter et al., 2015 | | |
Strain, strain background (virus) | rVSV eGFP HTNV Gn/Gc | Kleinfelter et al., 2015 | | |
Strain, strain background (virus) | rVSV eGFP SEOV Gn/Gc | Jangra et al., 2018 | | |
Strain, strain background (virus) | rVSV eGFP DOBV Gn/Gc | Slough et al., 2019 | | |
Strain, strain background (virus) | rVSV eGFP MPRLV Gn/Gc | Jangra et al., 2018 | | |
Strain, strain background (virus) | rVSV eGFP PHV Gn/Gc | Jangra et al., 2018 | | |
Strain, strain background (virus) | rVSV mNeongreen-P PUUV-Gn/Gc | Kerkman et al., 2019, This study | | Laboratory of K. Chandran |
Strain, strain background (virus) | rVSV mNeongreen-P CHOV Gn/Gc | This study | GenBank # KT983772.1 | Laboratory of K. Chandran/VSV antigenome plasmid (Whelan et al., 1995), plasmids expressing T7 polymerase and VSV N, P, M, G, and L (Witko et al., 2006) |
Strain, strain background (virus) | rVSV-EBOV/Mayinga GP (EBOV/H.sap-tc/COD/76/ Yambuku-Mayinga) | Wong et al., 2010 | | |
Strain, strain background (Hantavirus) | ANDV isolate Chile-9717869 | N/A | | |
Strain, strain background (Hantavirus) | HTNV isolate 76–118 | N/A | | |
Strain, strain background (Hantavirus) | PUUV isolate Sotkamo | N/A | | |
Strain, strain background (Hantavirus) | SNV isolate SN77734 | Botten et al., 2000 | | |
Strain, strain background (Hantavirus) | PHV | N/A | | Laboratory of K. Chandran |
Cell line (H. sapiens) | 293T | ATCC | Cat. # CRL-3216 | |
Cell line (H. sapiens) | HUVEC | Lonza | Cat. # C2517A | Primary cell |
Cell line (H. sapiens) | TIME (endothelial cell line) | ATCC | Cat. # CRL-4025 | |
Cell line (C. aethiops) | Vero | CCL-81 | Cat. # CCL-81 | |
Genetic reagent (H. sapiens) | TIME PCDH1 KO (endothelial cell line) | This study | | Laboratory of K. Chandran/Lentiviral transduction/lentiCRISPR v2-sgRNA PCDH1 |
Genetic reagent (H. sapiens) | TIME ITGB3 KO (endothelial cell line) | This study | | Laboratory of K. Chandran/Lentiviral transduction/lentiCRISPR v2-sgRNA ITGB3 |
Genetic reagent (H. sapiens) | TIME ITGB1 KO (endothelial cell line) | This study | | Laboratory of K. Chandran/Lentiviral transduction/lentiCRISPR v2-sgRNA ITGB3 |
Genetic reagent (H. sapiens) | TIME DAF KO (endothelial cell line) | This study | | Laboratory of K. Chandran/Lentiviral transduction/lentiCRISPR v2-sgRNA DAF |
Genetic reagent (H. sapiens) | TIME PCDH1/ITGB3 KO (endothelial cell line) | This study | | Laboratory of K. Chandran/TIME PCDH1 KO + Lentiviral transduction/lentiCRISPR v2-sgRNA ITGB3 |
Genetic reagent (H. sapiens) | TIME PCDH1/DAF KO (endothelial cell line) | This study | | Laboratory of K. Chandran / TIME PCDH1 KO + Lentiviral transduction/ lentiCRISPR v2-sgRNA DAF |
Genetic reagent (H. sapiens) | TIME ITGB3/DAF KO (endothelial cell line) | This study | | Laboratory of K. Chandran / TIME ITGB3 KO + Lentiviral transduction/ lentiCRISPR v2-sgRNA DAF |
Genetic reagent (H. sapiens) | TIME PCDH1 KO + PCDH1 (endothelial cell line) | This study | Canonical isoform Q08174-1 (Uniprot) | Laboratory of K. Chandran / Retroviral transduction/ pBabe-PCDH1 |
Genetic reagent (H. sapiens) | TIME ITGB3 KO + ITGB3 (endothelial cell line) | This study | Canonical isoform P05106-1 (Uniprot) | Laboratory of K. Chandran / Retroviral transduction/ pBabe-ITGB3 |
Genetic reagent (H. sapiens) | TIME DAF KO + DAF (endothelial cell line) | This study | Canonical isoform P08174-1 (Uniprot) | Laboratory of K. Chandran / Retroviral transduction/ pBabe-DAF |
Antibody | AF480-α-Human-vWF (Rabbit monoclonal) | Abcam | Cat. # ab195028 | Flow 1:250 |
Antibody | PeCy7-α-Human-PECAM (Mouse monoclonal) | BD | Cat. # 563651 | Flow 1:1000 |
Antibody | AF 647-α-Human- β3-Integrin (Mouse monoclonal) | Biolegends | Cat. # 336407 | Flow 1:200 |
Antibody | PE-α-Human-DAF (Mouse monoclonal) | BD | Cat. # 555694 | Flow 1:1600 |
Antibody | APC-α-Human-β1-Integrin (Mouse monoclonal) | BD | Cat. # 59883 | Flow 1:20 |
Antibody | α–Human-PCDH1 mAb 3305 (human monoclonal) | Jangra et al., 2018 | | Flow 1:200 |
Antibody | Convalescent serum (human polyclonal) | Stoltz et al., 2007 | | IF: 1:40 |
Antibody | AF488-α–-Human IgG (Goat polyclonal) | Invitrogen | Cat. # A-11013 | Flow 1:200 |
Antibody | AF594-α–Human IgG (Goat polyclonal) | Invitrogen | Cat. # A-11014 | IF 1:500 |
Antibody | AF594-α-Mouse IgG (Goat polyclonal) | Invitrogen | Cat. # A32742 | IF 1:500 |
Antibody | AF488-α-Rabbit IgG (Goat polyclonal) | Invitrogen | Cat. # A-11008 | IF 1:500 |
Antibody | α–Human PCDH1 (Mouse monoclonal) | Santa Cruz | Cat. # sc-81816 | WB 1:200 |
Antibody | α–Human β3 Integrin (Rabbit polyclonal) | Cell Signaling | Cat. # 4702 | WB 1:300 |
Antibody | α–Human-DAF (Mouse monoclonal) | Santa Cruz | Cat. # NaM16-4D3 | WB 1:200 |
Antibody | α–Human β Actin (Mouse monoclonal) | Santa Cruz | Cat. # sc-47778 | WB 1:300 |
Antibody | IRDye 680LT α-Mouse (Goat polyclonal) | LI-COR | Cat. # 926–68020 | WB 1:10,000 |
Antibody | IRDye 680LT Goat α-Rabbit IgG 680 (Goat polyclonal) | LI-COR | Cat. # 926–68021 | WB 1:10,000 |
Antibody | α-hantavirus nucleocapsid B5D9 (Mouse monoclonal) | Progen | Cat. # B5D9-C | IF 1:50 |
Antibody | α-HTNV nucleoprotein NR-12152 (Rabbit polyclonal) | BEI resources | Cat. # NR-12152 | IF 1:500 |
Recombinant DNA reagent | pBabe-puro (plasmid) | Morgenstern and Land, 1990 | Addgene plasmid # 1764 | |
Recombinant DNA reagent | pBabe-PCDH1 (plasmid) | Jangra et al., 2018 | | |
Recombinant DNA reagent | pBabe-ITGB3 (plasmid) | This study | | Laboratory of K. Chandran |
Recombinant DNA reagent | pBabe-DAF (plasmid) | This study | | Laboratory of K. Chandran |
Recombinant DNA reagent | lentiCRISPR v2 (plasmid) | Sanjana et al., 2014; Shalem et al., 2014 | | |
Recombinant DNA reagent | lentiCRISPR v2-sgRNA PCDH1 (plasmid) | Jangra et al., 2018 | | Laboratory of K. Chandran/5′-GTTTGAGCGGCCCTCCTATGAGG-3′ /PAM sequence is underlined but not included in oligos |
Recombinant DNA reagent | lentiCRISPR v2-sgRNA DAF (plasmid) | This study | | Laboratory of K. Chandran / 5′-CCCCCAGATGTACCTAATGCCCA-3′ / PAM sequence is underlined but not included in oligos |
Recombinant DNA reagent | lentiCRISPR v2-sgRNA ITGB3 (plasmid) | This study | | Laboratory of K. Chandran / 5′-CCACGCGAGGTGTGAGCTCCTGC-3′ /PAM sequence is underlined but not included in oligos |
Recombinant DNA reagent | lentiCRISPR v2-sgRNA ITGB1 (plasmid) | This study | | Laboratory of K. Chandran/5′-AATGTAACCAACCGTAGCAAAGG-3′ /PAM sequence is underlined but not included in oligos |
Sequence-based reagent | SNV degenerate primers | This study | | Laboratory of S. Bradfute/SNV F: CAgCTgTgTCTgCATTggAgAC SNV R: TARAgYCCgATggATTTCCAATCA |
Sequence-based reagent | SNV probe | This study | | Laboratory of S. Bradfute/ TMGB1: F-TCAAAACCTgTTgATCCA NFQ MGB |
Commercial assay or kit | pGEM-T Vector | Promega | Cat. # A3600 | |
Commercial assay or kit | VIC/TAMRA-dye probe | Applied Biosystems | Cat. # 4310881E | |
Commercial assay or kit | TaqMan Fast Advanced Master Mix | Applied Biosystems | Cat. # 4444556 | |
Commercial assay or kit | Foxp3/Transcription Factor Staining | Tonbo | Cat. # TNB-0607-KIT | |
Commercial assay or kit | Zombie NIR Fixable Viability Kit | BioLegend | Cat. # 423105 | Flow 1:4000 |
Software, algorithm | Cytation 5 Cell Imaging Multi-Mode Reader | Biotek | | |