1. Developmental Biology
  2. Stem Cells and Regenerative Medicine
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Adult stem cells and niche cells segregate gradually from common precursors that build the adult Drosophila ovary during pupal development

  1. Amy Reilein  Is a corresponding author
  2. Helen V Kogan
  3. Rachel Misner
  4. Karen Sophia Park
  5. Daniel Kalderon  Is a corresponding author
  1. Columbia University, United States
Research Article
  • Cited 1
  • Views 977
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Cite this article as: eLife 2021;10:e69749 doi: 10.7554/eLife.69749

Abstract

Production of proliferative Follicle Cells (FCs) and quiescent Escort Cells (ECs) by Follicle Stem Cells (FSCs) in adult Drosophila ovaries is regulated by niche signals from anterior (Cap Cells, ECs) and posterior (polar FCs) sources. Here we show that ECs, FSCs and FCs develop from common pupal precursors, with different fates acquired by progressive separation of cells along the AP axis and a graded decline in anterior cell proliferation. ECs, FSCs and most FCs derive from Intermingled Cell (IC) precursors interspersed with germline cells. Precursors also accumulate posterior to ICs before engulfing a naked germline cyst projected out of the germarium to form the first egg chamber and posterior polar FC signaling center. Thus, stem and niche cells develop in appropriate numbers and spatial organization through regulated proliferative expansion together with progressive establishment of spatial signaling cues that guide adult cell behavior, rather than through rigid early specification events.

Data availability

All data generated or analysed during this study are included in the manuscript and supporting files. Source data files are provided for all graphical data.

Article and author information

Author details

  1. Amy Reilein

    Columbia University, New York, United States
    For correspondence
    areilein@gmail.com
    Competing interests
    The authors declare that no competing interests exist.
  2. Helen V Kogan

    Columbia University, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
  3. Rachel Misner

    Columbia University, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
  4. Karen Sophia Park

    Columbia University, New York, United States
    Competing interests
    The authors declare that no competing interests exist.
  5. Daniel Kalderon

    Columbia University, New York, United States
    For correspondence
    ddk1@columbia.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-2149-0673

Funding

National Institutes of Health (GM079351)

  • Daniel Kalderon

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Claude Desplan, New York University, United States

Publication history

  1. Preprint posted: June 26, 2020 (view preprint)
  2. Received: April 24, 2021
  3. Accepted: September 29, 2021
  4. Accepted Manuscript published: September 30, 2021 (version 1)
  5. Version of Record published: October 22, 2021 (version 2)
  6. Version of Record updated: November 3, 2021 (version 3)

Copyright

© 2021, Reilein et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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