(A, B) Larval CNS at 100 AEL with glial expression of GFP+ GFP or Rasv12+ GFP was probed for F-actin (white), Repo (red), and DAPI (blue). The yellow dashed lines mark the boundary of glial …
The Drosophila larval CNS with glial expression of Rasv12+ GFP (green) was probed for Repo (red). The glial neoplasia tissue and non-tumor glial area are marked with cyan and white dashed lines, …
(A, B) Larval CNS at 100 AEL with glial expression of Rasv12+lacZ, Rasv12+NmnatRNAi, lacZ+Rasv12, PC+Rasv12, PCWR+Rasv12, and PD+Rasv12 was probed for F-actin (green), Repo (red), DAPI (blue), and …
Quantification of NMNAT intensity in glial cells or glial neoplasia area. Flies were expressing lacZ+lacZ or Rasv12+NmnatRNAi. Data are presented as median ± quartiles, n≥3. Significance level was …
Brains of two DAE flies with glial expression of GFP+GFP or GFP+NmnatRNAi were probed for Repo (red), Nmnat (white), and DAPI (blue). The third and fourth rows are high magnification of the boxed …
(A) Diagram of the continuous coupled enzyme assay where NAD+ synthesized by NMNAT is reduced to NADH by alcohol dehydrogenase (ADH). The production of NADH is measured by absorbance at 340 nm. …
(A, D) The xCELLigence real-time cell analysis assay was used to monitor the growth index of T98G cells after NMNAT knockdown by transfecting siNMNAT1 or siNMNAT2, or after NMNAT overexpression by …
siRNA sequences for NMNAT1/2 knockdown and primer sequences for PCR.
(A) The confluency of T98G cells is decreased after transfected with siNMNAT1 or siNMNAT2 96 hr. (B) Cell viability 96 hr after transfection was measured by an MTT assay. Data are presented as mean …
T98G cell viability was inhibited after knockdown NMNAT1 or NMNAT2.
(A–E) Cell cycle was detected by flow cytometry after T98G cells was transfected with siRNA Control, siNMNAT1-1, siNMNAT1-2, siNMNAT2-1, and siNMNAT2-2, respectively. (F) Quantification of cells in …
SVG p12 is human glial cells. T98G and U87MG are human glioma cells. Proteins were extracted from cells were probed for NMNAT1, NMNAT2, and β-actin and quantification. NMNAT2 overexpression from …
NMNAT protein was upregulated in human glioma cells.
(A) Glial neoplasia from files expressing lacZ, PC, PCWR, or PD were probed for Repo (red), F-actin (green), DAPI (blue), and caspase-3 (gray). The top row shows the whole glial neoplasia area. The …
(A) Diagram of caspase pathway in mammalian and Drosophila. (B) Flies with Rasv12+lacZ, Rasv12+Diap1, Rasv12+p35, Rasv12+DCP1 RNAi, and Rasv12+DroncRNAi were probed for Repo (green) and F-actin …
(A) T98G cells were transfected with siNMNAT1 or siNMNAT2 and stained with DAPI (blue) and caspase-3 (white). (B) T98G cells were transfected with DsRed (red), DsRed-NMNAT1 (red), or DsRed-NMNAT2 …
NMNAT decreased caspase-3 activation in human glioma cells.
Proteins were extracted from T98G cells transfected with siRNA (left), or plasmids and treated with cisplatin for 8 hr (right) for Western blot analysis. P32 was considered as pro-caspase-3. P17/19 …
U87MG cells were treated with CDDP and probed for p53, caspase-3, and β-actin. NMNAT, nicotinamide mononucleotide adenylyltransferase.
Cleaved Ccaspase-3 was reduced after NMNAT overexpression.
(A) Glial neoplasia from flies expressing lacZ, PC, PCWR, or PD were stained with H2Av (red), Repo (green), and F-actin (magenta). The second and third rows are high magnification of the boxed areas …
(A) Flies expressing Rasv12 and CD8GFP were treated with DMSO or p53 inhibitor, respectively, stained with cleaved caspase-3 (red) and DAPI (blue). The first column is the whole CNS of flies. White …
(A) Proteins were extracted from U87MG and T98G cells transfected with siNMNAT1/2 after knockdown of p53 or GFP by shRNA lentivirus transduction for Western blot analysis. β-actin was used as an …
p53 depletion rescued NMNAT knockdown-induced caspase-3 activation in glioma.
(A) Proteins were extracted from U87MG and T98G cells transfected with siRNA for Western blot analysis. β-actin was used as an internal control. (B) Quantification of cleaved caspase-3 in Western …
p53 depletion rescued NMNAT knockdown-induced caspase-3 activation in glioma.
(A) Proteins were extracted from T98G cells transfected with plasmids for Western blot and dot blot analyses using anti-PAR antibody. (B) Quantification of PAR in Western blot analysis. Data are …
NMNAT interacts with p53 and PARP1 and upregulates PARylation.
Proteins were extracted from T98G cells transfected with siRNA for dot blot analysis using anti-PAR antibody and quantification. Data are presented as mean ± SD, n=3. Significance level was …
Protein samples extracted from U87MG cells transfected with DsRed, DsRed-NMNAT1, or DsRed-NMNAT2 were immunoprecipitated (IP) with a p53 antibody and subjected to immunoblot (IB) analysis for p53, …
NMNAT interacts with p53 in U87MG.
(A) Protein samples extracted from T98G cells transfected with DsRed, DsRed-NMNAT1, or NMNAT2 were immunoprecipitated (IP) with a p53 antibody and probed for acetyl-p53 and SIRT1. Red asterisks (*) …
NMNAT upregulates SIRT1 and reduces acetylation of p53.
NMNAT upregulates SIRT1 and reduces acetylation of p53.
U87MG cells were treated with CDDP and probed for p53, acetyl-p53, and tubulin. NMNAT, nicotinamide mononucleotide adenylyltransferase.
Acetyl-p53 was reduced in U87MG cells under NMNAT overexpression conditions.
(A, B) LGG (lower grade glioma) data set (GEPIA). Survival curves for patients with tumors expressing median (top 50% vs. bottom 50%) NMNAT1 levels (A), or top 10% versus bottom 10% levels (B), with …
Alteration of human NMNAT1 and NMNAT2 was queried in TCGA database (https://www.TCGA.com). 1522 cases with altered NMNAT1 and 931 cases with altered NMNAT2 across 32 projects are shown. NMNAT, …
Alteration of human NMNAT1 and NMNAT2 was queried in cBioPortal database (https://www.cbioportal.com) separately. 10,967 samples in 35 cancer types are shown. NMNAT, nicotinamide mononucleotide …
org dataset
Manuscript | Cancer | Cell Line | Gene | Gene effect (CERES) | Expression (Log2(TPM+1)) |
---|---|---|---|---|---|
Liu et al. (2021). bioRxiv.(this manuscript) | Glioma | T98G | NMNAT1NMNAT2 | -0.427-0.0177 | 2.872.29 |
U87MG | NMNAT1NMNAT2 | -0.1590.156 | 3.15.74 | ||
Challa et al. (2021). Cell | Ovarian | OVCAR3 | NMNAT1NMNAT2 | -0.2190.0989 | 3.264.18 |
Shi et al. (2021).Science Advances | Acute myeloid leukaemia | MOLM13 | NMNAT1NMNAT2 | -0.33-0.00213 | 2.310.189 |
OCIAML2 | NMNAT1NMNAT2 | 0.0588 0.0624 | 0.8881.52 |
Original data file.