Boosting targeted genome editing using the hei-tag
Abstract
Precise, targeted genome editing by CRISPR/Cas9 is key for basic research and translational approaches in model and non-model systems. While active in all species tested so far, editing efficiencies still leave room for improvement. The bacterial Cas9 needs to be efficiently shuttled into the nucleus as attempted by fusion with nuclear localization signals (NLSs). Additional peptide tags such as FLAG- or myc-tags are usually added for immediate detection or straightforward purification. Immediate activity is usually granted by administration of preassembled protein/RNA complexes. We present the ‘hei-tag (high efficiency-tag)’ which boosts the activity of CRISPR/Cas genome editing tools already when supplied as mRNA. The addition of the hei-tag, a myc-tag coupled to an optimized NLS via a flexible linker, to Cas9 or a C-to-T (cytosine-to-thymine) base editor dramatically enhances the respective targeting efficiency. This results in an increase in bi-allelic editing, yet reduction of allele variance, indicating an immediate activity even at early developmental stages. The hei-tag boost is active in model systems ranging from fish to mammals, including tissue culture applications. The simple addition of the hei-tag allows to instantly upgrade existing and potentially highly adapted systems as well as to establish novel highly efficient tools immediately applicable at the mRNA level.
Editor's evaluation
This manuscript describes the addition of a short tag on the Cas9 nuclease as a means to improve genome editing efficiency. Importantly, the authors have tested their approach on several genomic targets, model organisms, and Cas9 derivative engineering tools. Overall, these findings support the possible general applicability of this tag for improving the outcomes of a wide range of modern Cas9 based applications, including Base Editors. Adding to a recent report of improving Prime Editing by optimising the NLS, this paper reinforces the notion that there is still unexplored space in altering genome engineering activity in a modular way.
https://doi.org/10.7554/eLife.70558.sa0eLife digest
The genetic code stored within DNA provides cells with the instructions they need to carry out their role in the body. Any changes to these genes, or the DNA sequence around them, has the potential to completely alter how a cell behaves.
Scientists have developed various tools that allow them to experimentally modify the genome of cells or even entire living organisms. This includes the popular Cas9 enzyme which cuts DNA at specific sites, and base editors which can precisely change bits of genetic code without cutting DNA. While there are lots of Cas9 enzymes and base editors currently available, these often differ greatly in their activity depending on which cell type or organism they are applied to.
Finding a tool that can effectively modify the genome of an organism at the right time during development also poses a challenge. All the cells in an organism arise from a single fertilized cell. If this cell is genetically edited, all its subsequent daughter cells (which make up the entire organism) will contain the genetic modification. However, most genome editing tools only work efficiently later in development, resulting in an undesirable mosaic organism composed of both edited and non-edited cells.
Here, Thumberger et al. have developed a new ‘high efficiency-tag’ (also known as hei-tag for short) that can enhance the activity of gene editing tools and overcome this barrier. The tag improves the efficiency of gene editing by immediately shuttling a Cas9 enzyme to the nucleus, the cellular compartment that stores DNA. In all cases, gene editing tools with hei-tag worked better than those without in fish embryos and mouse cells grown in the laboratory. When Cas9 enzymes connected to a hei-tag were injected into the first fertilized cell of a fish embryo, this resulted in an even distribution of edited genes spread throughout the whole organism.
To understand how a gene affects an organism, researchers need to be able to edit it as early in development as possible. Attaching the ‘hei-tag’ to already available tools could help boost their activity and make them more efficient. It could also allow advances in medical research aimed at replacing faulty genes with fully functioning ones.
Introduction
In the last decade, the CRISPR/Cas9 system and its derivatives facilitated and revolutionized genome editing across all phyla (Nidhi et al., 2021). The efficiency of editing crucially depends on the on-site activity of the particular Cas9 enzymes used (usually Streptococcus pyogenes Cas9, SpCas9) in the nucleus. State-of-the-art Cas9 variants differ by peptide tags added to the N- and C-termini of the respective endonuclease resulting in reported different activities (Liu et al., 2021; Zhang et al., 2014). Employed tags usually comprise diverse nuclear localization signals (NLSs) and epitope tags (e.g. FLAG, Myc, HA) for potential protein purification or visualization. To achieve nuclear localization of the Cas9 enzyme, the monopartite NLS originating from the SV40 large T-antigen (Kalderon et al., 1984) or a bipartite NLS discovered in Xenopus nucleoplasmin is routinely employed (Dingwall et al., 1988). However, the nuclear localization activity of commonly used NLSs is tightly controlled during early development (Poon and Jans, 2005) and is first detectable during gastrulation. In fish embryos, an optimized artificial NLS (Inoue et al., 2016) (oNLS) facilitates prominent nuclear localization already immediately after fertilization, while the SV40 NLS acts most prominently much later and facilitates nuclear localization approximately at the 1000-cell stage. For high targeting efficiency with low mosaicism, a peak activity should be achieved in the zygote or at early cleavage stages. Here, we present the hei-tag, a short bipartite tag composed of a myc-tag and optimized NLSs at the N- and C-termini, that boosts Cas9 or cytosine-to-thymine (C-to-T) base editor-mediated targeted genome editing in organismo and cell culture.
Results
Assessing the genome editing efficiency requires a reliable and quantitative readout based on an apparent phenotype. We established a quantitative assay for loss-of-eye pigmentation to address the activity of different Cas9 variants in two teleost model systems, medaka (Oryzias latipes) and zebrafish (Danio rerio) covering a wide evolutionary distance of 200 million years (Furutani-Seiki and Wittbrodt, 2004). Our assay on retinal pigmentation provides a highly reproducible quantitative readout for the loss of the conserved transporter protein oculocutaneous albinism type 2 (oca2), required for melanin biosynthesis (Figure 1a). Only its bi-allelic inactivation results in the loss of pigmentation of eyes and skin (Lischik et al., 2019). A prominent knock-out phenotype thus can either result from a single to few early events, or from many events at subsequent developmental stages. Although phenotypically indifferent, the allele variance (genetic mosaicism) reflects the time point of action.
State-of-the-art protocols employ high concentrations of Cas9 and respective sgRNAs to ensure efficient on-site editing. To facilitate uniform Cas9 action, we followed our successful mRNA injection protocol (Gutierrez-Triana et al., 2018). One-cell stage medaka embryos were co-injected with sgRNAs targeting the oca2 gene (OlOca2 T1, T2) together with mRNA encoding a Cas9 endonuclease and mRNA encoding the green fluorescent protein (GFP) as injection tracer. Injected embryos were fixed at 4.5 days post fertilization (dpf) (Iwamatsu, 2004) well after the onset of pigmentation in control injections and subjected to image analysis (Figure 1b). In brief, the eyes were segmented, (residual) pigmentation was thresholded (Figure 1c–c’) and quantified according to mean gray values (0, i.e. fully pigmented, 255, i.e. completely unpigmented, Figure 1d).
We first established the base activity level for the assay at standard conditions with high molar excess (150 ng/µl concentration) and determined the activity of a Cas9 variant codon optimized for zebrafish, that is, a Cas9 carrying an SV40 NLS at the N- and C-terminus (nls-zCas9-nls, hereinafter: zCas9, Plasmid #47929 Addgene, Jao et al., 2013). The analysis of medaka oca2 knock-out embryos injected with zCas9 revealed bi-allelic inactivation events of the oca2 gene, yet with a strong overall variability as apparent by patchy unpigmented domains in the eyes (median of mean gray values = 134.5 compared to uninjected controls, median = 0.4; Figure 1d). This patchy distribution of small, unpigmented areas indicated that bi-allelic targeting occurred only in few cells at later stages of development. To address whether different peptide domains (NLSs, Myc-tag, amino acid linkers) flanking the Cas9 enzyme enhance the targeting efficiency, we performed a permutation screen with Cas9 variants carrying these domains at different positions, which resulted in the identification of the ‘hei-tag’ (Figure 1—figure supplement 1). The hei-tag comprises a myc-tag connected via a flexible linker to an oNLS at the N-terminus complemented by a second oNLS fused to the C-terminus of a mammalian codon-optimized Cas9 (see Supplementary file 1 for sequence) and in this conformation displayed highest editing activity. Any alteration of those domains in relative order or sequence negatively impacted on editing efficiency compared to the hei-tag (Supplementary file 2).
When assessing the activity of the resulting heiCas9 at high molar excess (standard conditions, 150 ng/µl), heiCas9 displayed a 70% increase in bi-allelic targeting efficiency vs. the reference zCas9 (median zCas9 = 134.5, heiCas9 = 225.3; Figure 1d) in medaka. Embryos co-injected with heiCas9 mRNA and sgRNAs against oca2 essentially lost pigmentation. The observed absence of pigmentation argues for an early time point of action due to high activity and efficient nuclear translocation of the tagged heiCas9 variant already at the earliest cleavage stages. In developing organisms, the time point of genome editing essentially impacts on the allele variance, that is, the number of alleles established by the targeting attempt. To immediately provide a functional editing machinery, preassembled ribonucleoproteins (RNPs) containing Cas9 protein and guide RNA are popular, employing high molar excess/high concentrations of Cas9 (Kroll et al., 2021; Wu et al., 2018). Strikingly, the editing efficiency of injected heiCas9 mRNA was fully comparable to such RNP approaches (Figure 1d, Figure 1—figure supplement 2).
To address whether the enhancement by hei-tag fusion to Cas9 is applicable to different models, we next compared the activities of the zCas9 and heiCas9 in a second, evolutionarily distant fish species D. rerio (zebrafish) targeting the orthologous oca2 gene (sgRNAs DrOca2 T1, T2; Hammouda et al., 2019). Injected and control embryos were fixed well after the onset of pigmentation at 2.5 dpf (Kimmel et al., 1995; Figure 1e–f) and subjected to the quantitative assay for eye pigmentation described above. Taking the activity of zCas9 as base level (median = 14.7), heiCas9 delivered an outstanding targeting efficiency (median = 254.6), reflecting a 17-fold increase (p = 2.1e-56) (Figure 1g, Figure 1—figure supplement 2). Similar to the results in medaka, yet even more pronounced, nearly unpigmented embryos were obtained with the heiCas9, arguing for highly efficient, early targeting. Taken together, addition of the hei-tag to a mammalian codon-optimized Cas9 resulted in the highly efficient heiCas9, which boosted the targeting efficiency 17-fold, even when used at saturating concentrations. It prominently inactivated both alleles of the targeted oca2 locus, with a putatively early onset of action upon injection of heiCas9 mRNA and the respective sgRNAs at the one-cell stage.
To address whether the high targeting efficiency of heiCas9 was conveyed by the high molar excess employed or was possibly restricted to the oca2 locus, we turned to a multiplexing regime at 10-fold reduced concentrations of the Cas9 variants employed. We targeted four different genomic loci with four different sgRNAs: exonic targeting of oca2 (OlOca2 T2), targeting of the start codon of the retina-specific transcription factor 2 (rx2; Stemmer et al., 2015), and the crystallin alpha a (cryaa; Stemmer et al., 2015) as well as intronic targeting of rx3 (Zilova et al., 2021). Medaka one-cell stage embryos were co-injected with a mix of 12.5 ng/µl per sgRNA, the 10-fold reduced (15 ng/µl) zCas9 or heiCas9 mRNA and 20 ng/µl mCherry mRNA as injection tracer.
For each multiplexing experiment, the genomic DNA of three pools each containing eight randomly picked crispants was extracted at 4 dpf and subjected to allele-specific genotyping via Illumina sequencing. In the multiplexing approaches, a total of 823,898 reads for the zCas9 and 824,817 reads for the heiCas9, compared to 711,739 control reads, were analyzed (Supplementary file 3, Figure 2—figure supplement 1). In all cases, heiCas9 performed dramatically better than the reference zCas9 (Figure 2a; mean percentage of modified alleles zCas9 [black dots] vs. heiCas9 [red dots]: OlOca2: 3.38% vs. 54.59%, p = 0.026; OlRx2: 20.82% vs. 95.85%, p = 3.2e-06; OlRx3: 16.61% vs. 49.36%, p = 0.0041; OlCryaa: 83.50% vs. 98.44%, p = 0.039). Strikingly, although the overall targeting efficiency was consistently higher as reflected by the high percentage of edited alleles (Figure 2a), at the same time the allele variance was reduced in all cases when using heiCas9 (Figure 2b; mean percentage of allele variance: zCas9 [black hollow dots] vs. heiCas9 [red hollow dots]: OlOca2: 20.71% vs. 12.87%, p = 0.025; OlRx2: 15.63% vs. 7.86%, p = 7.6e-06; OlRx3: 17.91% vs. 12.75%, p = 0.00021; OlCryaa: 10.17% vs. 8.74%, p = 0.22). This reduced allele variance for all multiplexed loci indicates an early editing by heiCas9. Given this and the overall higher targeting efficiency in all loci analyzed in the multiplexing approach, heiCas9 outperformed zCas9. It resulted in a massive performance boost, which was partially masked at saturating conditions, and now became fully apparent. The high efficiency of heiCas9 thus allows efficient editing at low concentrations with the potential to reduce off-target effects. Whether this putative reduction of off-targets is (over-)compensated by the efficient nuclear localization needs to be assessed by whole-genome sequencing approaches in the future.
While the early onset of action is required for uniform editing in developing organisms, cell culture approaches demand efficient translocation of the sgRNA/Cas9 complex in a large number of cells. To validate the range of action on the one hand and to address the relevance of the hei-tag in a mammalian setting, we expanded the scope of the analysis to mammalian cell culture. We focused on mRNA-based assays and compared the activity of heiCas9 to state-of-the-art Cas9 variants, that is, the commercially available GeneArt CRISPR nuclease as well as a mammalian codon-optimized Cas9 (JDS246-Cas9, Addgene #43861) in mouse SW10 cells. We assessed the respective genome editing efficiencies by independent and complementary tools, the Tracking of Indels by Decomposition (TIDE) analysis (Brinkman et al., 2014) as well as by Inference of CRISPR Editing (ICE) (Hsiau et al., 2018). Both approaches decompose the mixed Sanger reads of PCR products spanning the CRISPR target site and compute an efficiency score as well as the distribution of expected indels. To target the murine Periaxin (Prx) locus, mouse SW10 cells were co-transfected with MmPrx crRNA/ATTO-550-linked tracrRNA and the mRNAs of either JDS246-Cas9, GeneArt CRISPR nuclease, or heiCas9. The Prx locus was PCR amplified and sequenced. Similar to targeting in organismo, heiCas9 also exhibited the highest genome editing efficiency when compared to JDS246-Cas9 (TIDE: 123.6%, ICE: 113%) and GeneArt CRISPR nuclease (TIDE: 123.1%, ICE: 111%) in mammalian cell culture (Figure 3, Figure 3—figure supplement 1, R2 > 0.9 (TIDE) and >0.9 (ICE) for all mRNAs tested). Notably, the KO-score efficiencies (ICE) amounted to 173% compared to JDS246-Cas9 and to 167% compared to GeneArt CRISPR nuclease, indicating higher abundance of frameshifts (Hsiau et al., 2018) at this genomic locus.
Remarkably, heiCas9-transfected cells showed a highly increased number of mutant alleles with an increased abundance of a 26 nt deletion when compared to GeneArt CRISPR nuclease and JDS246-Cas9 (Figure 3—figure supplement 1).
Given the observed boosting of Cas9 activity by the simple addition of the hei-tag, we next tested if the hei-tag also improves further Cas9-based techniques. Base editing is an increasingly applied method with a potential for therapeutics (Antoniou et al., 2021). Base editors are composed of a modified Cas9 that only nicks one DNA strand and does not introduce a double-strand break (Cas9 nickase or Cas9n) and a nucleotide deaminase for precisely targeted nucleotide editing (Anzalone et al., 2020). To increase the efficiency of base editors, several iterative rounds of optimization of the employed deaminases and linkers have been undertaken, yielding optimal performance with the newest variants (Carrington et al., 2020; Cornean et al., 2022; Rosello et al., 2021; Zhao et al., 2020). To investigate if the addition of the hei-tag provides an easy and straightforward alternative route for increasing the activity of a nuclear protein of interest, we selected a C-to-T base editor version with intermediate efficiency (BE4-Gam Komor et al., 2017) to introduce non-sense or severe miss-sense mutations into the pigmentation gene oca2. We employed our tool ACEofBASEs (Cornean et al., 2022) to design and evaluate sgRNA target sites that introduce non-synonymous codon mutations and/or pre-mature STOP codons upon editing of the respective open reading frame (ORF). We compared three different sgRNAs (OlOca2 T1, T3, and T4) employing the original BE4-Gam and the hei-tag fused variant (heiBE4-Gam). In the oca2 ORF, the transition of cytosines 766, 922, and 997 to thymine all convert the respective codon to a pre-mature STOP (OlOca2 T3: C766T, leading to Q256*; OlOca2 T4: C922T, leading to Q308*; OlOca2 T1: C995-997T, leading to T332I and Q333*). Again, the loss of pigmentation was used as proxy for bi-allelic targeting efficiency following medaka one-cell stage injections with either one of the three sgRNAs (OlOca2 T1, T3, or T4, 30ng/µl) as well as 150ng/µl mRNA of either BE4-Gam or heiBE4-Gam. Screening and analysis was performed at 4.5 dpf as described above. For each sgRNA employed, heiBE4-Gam resulted in more pronounced loss of pigmentation in comparison to BE4-Gam (Figure 4a; control median = 0.0; medians BE4-Gam vs. heiBE4-Gam: OlOca2 T1, 0.6 vs. 28.0, p = 1.737e-20; OlOca2 T3, 0.0 vs. 0.8, p = 0.0471; OlOca2 T4, 93.8 vs. 170.1, p = 5.215e-12). Quantification of Sanger sequencing reads confirmed an increase of all C-to-T transitions at the OlOca2 T1 target site when heiBE4-Gam was used (74.1% ± 8.9% for heiBE4-Gam vs. 44.2% ± 6.8% for BE4-Gam; Figure 4—figure supplement 1, three replicates containing five randomly picked embryos each). In particular, the C997T transition introducing a pre-mature STOP codon was increased 1.7-fold (i.e. 68% in heiBE4-Gam vs. 41% in BE4-Gam) in case of heiBE4-Gam (Figure 4b and c).
In conclusion, using the hei-tag to extend the ORFs of a mammalian codon-optimized SpCas9 or a C-to-T base editor (BE4-Gam) severely enhanced the respective genome targeting efficiency.
Discussion
While the use of the optimized NLS in the hei-tag explains the earlier and better performance of the hei-tagged versions of Cas9 and base editors in developing organisms, the impact of the specific topology of domains contained in the hei-tag remains elusive. It is speculated that the addition of certain peptide tags influences the efficacy and specificity of the fused protein of interest, due to their different isoelectric points and charge distributions (Zhang et al., 2014). Interestingly, our permutation screen demonstrated that although comprising the exact same peptides (for instance, compare MFO-Cas9-O [heiCas9] vs. OMF-Cas9-O and MSF-Cas9-S vs. SMF-Cas9-S in Figure 1—figure supplement 1), position of the particular tags relative to each other conveyed different genome editing efficiencies.
The hei-tag renders the resulting heiCas9 into a highly efficient endonuclease with broad applicability overcoming the limitations of current SpCas9 variants by dramatically increasing the efficiency of targeted genome editing in organismo, as demonstrated in two evolutionarily distant fish models, as well as in mouse cell culture. In those systems, heiCas9 leads to a high abundance of identical mutant alleles, important for testing specific hypotheses or introducing site-specific modifications by homology-directed repair (Gutierrez-Triana et al., 2018). Conversely, Cas9 variants without the hei-tag are better suited for targeted screening approaches since they introduce a large number of different mutant alleles. heiCas9 markedly increased the (bi-allelic) targeting rate alongside a decrease in allele variance, indicating a high targeting efficiency already at the earliest stages of development. Precedentially such early targeting in developing organisms was most of all reported using RNPs (Kroll et al., 2021; Wu et al., 2018), yet mRNA injection of heiCas9 is fully comparable to these protein approaches. The benefits of using mRNA over protein are apparent: new Cas9 variants can easily be generated and produced cost-efficiently by highly reproducible in vitro transcription, a standard method in molecular biology labs.
In light of the ever-expanding CRISPR tool kit, the addition of the hei-tag provides the means to boost current specialized and future variants, as the simple addition of the hei-tag sequence also potentiated the activity of a cytosine base editor, with heiBE4-Gam resulting in an overall increase of about 30% of C-to-T transition rates (Figure 4 and Figure 4—figure supplement 1). Taken together, the boosting activity of the hei-tag is neither limited by the species nor the approach, making it a powerful tweak to swiftly upgrade any specifically adapted Cas-based genome editing approach (Anzalone et al., 2020).
Materials and methods
Fish maintenance
Request a detailed protocolZebrafish (D. rerio) and medaka (O. latipes) fish were bred and maintained as previously described (Koster et al., 1997; Westerfield, 2000). The animal strains used in the present study were zebrafish AB/back and medaka Cab. All experimental procedures were performed according to the guidelines of the German animal welfare law and approved by the local government (Tierschutzgesetz §11, Abs. 1, Nr. 1, husbandry permit number 35-9185.64/BH Wittbrodt).
Cloning of Cas9 variants
Request a detailed protocolThe mammalian codon-optimized (Geneious 8.1.9, https://www.geneious.com) Cas9 sequence was gene-synthesized (GeneArt, ThermoFisher Scientific) as template for cloning the permutated peptide-tag Cas9 fusions (Supplementary file 2) using primers (Table 1) containing the sequences coding for a myc-tag (EQKLISEEDL), flexible or internal linkers and an SV40 (PKKKRKV) or optimized oNLS (PPPKRPRLD) (Inoue et al., 2016; Figure 1—figure supplement 1). Cloning into the pCS2+ plasmid (Rupp et al., 1994) (multiple cloning site extended for AgeI site downstream of BamHI site) was performed using AgeI and XbaI restriction sites included in the 5’ region of the forward or reverse primers, respectively. See Supplementary file 1 for full sequence of heiCas9. For consistent mRNA synthesis, the published myc-Cas9 (Zhang et al., 2014) (MSI-Cas9-Xl) was re-established with the pX330-U6-Chimeric_BB-CBh-hSpCas9 vector as template, primer-based exchange of the N-terminal FLAG tag with the myc-tag sequence and brought into pCS2+ (Rupp et al., 1994) using AgeI and XbaI restriction sites included in the 5’ region of the respective primers as well. pX330-U6-Chimeric_BB-CBh-hSpCas9 was a gift from Feng Zhang (Addgene plasmid #42230) (Cong et al., 2013).
Cloning of BE4-Gam and heiBE4-Gam
Request a detailed protocolBE4-Gam was subcloned from pCMV(BE4-Gam) (Addgene plasmid #100806, a gift from David Liu) (Komor et al., 2017) in a two-step process, first into pJET1.2 (Thermo Scientific), then into pGGEV4 (Kirchmaier et al., 2013) (Addgene plasmid #49284), by BamHI, EcoRV, and KpnI restriction sites to create pGGEV4(BE4-Gam). heiBE4-Gam was assembled into pCS2+ (Rupp et al., 1994) by NEBuilder HiFi DNA Assembly (NEB) with four inserts using Q5 polymerase PCR products (NEB): pCS2+ backbone, hei-tag fragment, Gam Mu-APOBEC1-Cas9n fragment, Cas9n-UGI fragment, 2xUGI-oNLS (see Table 2 for primers used).
sgRNA design
Request a detailed protocolAll sgRNAs for medaka (OlOca2, Rx2, Rx3, Cryaa) and zebrafish (DrOca2) were designed using the CCTop target predictor with standard parameters (Stemmer et al., 2015). The sgRNAs used for base editing (OlOca2 T1, T3, T4) were designed or evaluated using ACEofBASEs (Cornean et al., 2022) and selected for introducing a pre-mature STOP codon. The following target sites were used [PAM in brackets]: OlOca2 T1 (GAAACCCAGGTGGCCATTGC[AGG]), OlOca2 T2 (TTGCAGGAATCATTCTGTGT[GGG]), OlOca2 T3 (GATCCAAGTGGAGCAGACTG[AGG]), OlOca2 T4 (CACAATCCAGGCCTTCCTGC[AGG]) DrOca2 T1 (GTACAGCGACTGGTTAGTCA[TGG]), DrOca2 T2 (TAAGCACGTAGACTCCTGCC[AGG]), Rx2 (GCATTTGTCAATGGATACCC[TGG]), Cryaa (GGGAGAAGTGCTTGACATCC[AGG]), Rx3 (AGCAGAGCGCGCAAAGAACC[AGG]). OlOca2 T1, OlOca2 T2, and DrOca2 T1 were the same as in Hammouda et al., 2019, OlOca2 T3 was the same as in Lischik et al., 2019 (OCA2_4), OlRx2 and OlCryaa are from Stemmer et al., 2015, and OlRx3 is the same used in Zilova et al., 2021. Cloning of sgRNA templates was performed as described (Stemmer et al., 2015). Plasmid DR274 was a gift from Keith Joung (Addgene plasmid #42250) (Hwang et al., 2013).
In vitro transcription of mRNA pCS2+ constructs in this work were linearized using NotI-HF (NEB) except for zCas9 – linearized with HpaI (NEB). The pGGEV4(BE4-Gam) was linearized using SpeI-HF (NEB). mRNA was transcribed in vitro using the mMESSAGE mMACHINE SP6 transcription kit (ThermoFisher Scientific, AM1340). pCS2-nCas9n (zCas9) was a gift from Wenbiao Chen (Addgene plasmid #47929) (Jao et al., 2013). The JDS246-Cas9 was linearized with MssI FD (ThermoFisher Scientific) and transcribed in vitro using the mMESSAGE mMACHINE T7 Ultra Transcription Kit (ThermoFisher Scientific, AM1345). JDS246-Cas9 was a gift from Keith Joung (Addgene plasmid #43861). sgRNAs were synthesized using the MEGAscript T7 transcription kit (ThermoFisher Scientific, AM1334) after plasmid digestion with DraI FD (ThermoFisher Scientific).
Microinjection
Request a detailed protocolAll microinjections were performed at the one-cell stage. At standard concentrations, zebrafish and medaka zygotes were injected with 150 ng/µl Cas9 (variant) mRNA, Oca2 sgRNAs at 30 ng/µl, and H2B-GFP mRNA at 10 ng/µl as injection tracer. The multiplexing injection mixes contained 12.5 ng/µl per sgRNA (OlOca2 T2, Rx2, Rx3, Cryaa) and 15 ng/µl of either zCas9 or heiCas9 mRNA as well as 20 ng/µl mCherry mRNA as injection tracer. For the protein injections, 24 µM RNP mix (Kroll et al., 2021) was assembled in Cas9 buffer (20 mM Tris-HCl, 600 mM KCl, 20% glycerol; Wu et al., 2018) by mixing 61 µM Alt-R S.p. Cas9 Nuclease V3 (IDT) with 5710 ng of each sgRNA OlOca2 T1 and T2; 285.6 ng GFP mRNA were added as injection tracer. The mix was incubated for 5 min at 37°C and stored on ice until further dilution and injection. To obtain 5 µM RNPs (Wu et al., 2018), the 24 µM RNP mix was diluted in a 1:1 mixture of Cas9 buffer and nuclease-free water. Five µM RNP solution was further diluted in a 1:1 mixture of Cas9 buffer and nuclease-free water to obtain 1.765 µM RNPs.
For the base editing experiments, medaka zygotes were injected with BE4-Gam or heiBE4-Gam mRNA at 150 ng/µl, respective Oca2 sgRNA at 30 ng/µl, and GFP mRNA at 20 ng/µl as injection tracer. All injected embryos were maintained at 28°C in zebrafish medium (Westerfield, 2000) or medaka embryo rearing medium (ERM, 17 mM NaCl; 40 mM KCl; 0.27 mM CaCl2•2H2O; 0.66 mM MgSO4•7H2O, 17 mM HEPES).
Embryos were screened for GFP or mCherry expression 4–7 hr or 1 day after injection using a Nikon SMZ18 stereomicroscope, and uninjected specimens were discarded.
Image acquisition and phenotype analysis
Request a detailed protocolMedaka 4.5 dpf embryos (Iwamatsu, 2004) and zebrafish 2.5 dpf (Kimmel et al., 1995) embryos were fixed with 4% paraformaldehyde in 2× PTW (2× PBS pH 7.3, 0.1% Tween 20). Images of medaka embryos were acquired with the high content screening ACQUIFER Imaging Machine (DITABIS AG, Pforzheim, Germany). Images of zebrafish embryos were acquired with a Nikon digital sight DS-Ri1 camera mounted onto a Nikon Microscope SMZ18 and the Nikon Software NIS-Elements F version 4.0. Only properly developed embryos were included in the following analysis. Image analysis was performed with Fiji (Schindelin et al., 2012), that is, mean gray values were obtained on minimum intensity projections and locally thresholded (Phansalkar algorithm with parameters r = 20, p = 0.4, k = 0.4) pictures and elliptical selections for each individual eye. The mean gray value per eye was used for the boxplot and statistical analysis (pairwise comparisons using Wilcoxon rank sum test, Bonferroni corrected) in RStudio (Team, 2020).
Targeted amplicon sequencing via illumina
Request a detailed protocolThe multiplex approach was genotyped on DNA extractions of pools with each replicate containing eight randomly picked crispants per zCas9 or heiCas9 injection or six control specimens. DNA was prepared by grinding and lysis in DNA extraction buffer (0.4 M Tris/HCl pH 8.0, 0.15 M NaCl, 0.1% SDS, 5 mM EDTA, pH 8.0, 1 mg/ml proteinase K) at 60°C overnight. Proteinase K was inactivated at 95°C for 10 min and the solution was diluted 1:2 with nuclease-free water. For each DNA extraction, small libraries were constructed by PCR amplifying the four regions of interest (295–362 bp, OlOca2, rx2, rx3, cryaa) using locus-specific primers with 5’ partial illumina adapter sequences (Table 3) and Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs). PCR products were run on a 1% agarose gel, respective bands were excised and cleaned up using the Monarch DNA Gel Extraction Kit (New England Biolabs). PCR products from the same genomic DNA source were pooled to equilmolarity at 25 ng/µl and submitted to GeneWiz (Azenta Life Sciences) for sequencing (Amplicon-EZ: Illumina MiSeq, 2 × 250 bp sequencing, paired-end) obtaining between 48,018 and 96,899 reads per sample.
Analysis and plotting of next-generation sequencing data
Request a detailed protocolAmplicon sequencing data was analyzed with CRISPResso2 v.2.1.2 (Clement et al., 2019) using the default -n nhej parameters. Demultiplexing was achieved by mapping to the four different wild-type loci, respectively. Downstream analysis was conducted using R v.3.6.3 in R studio (Team, 2020) (package: ggplot2 Wickham, 2016), with data sourced from ‘CRISPResso_quantification_of_editing_frequency.txt’ output table. To determine the average read count per modified allele, the ‘Alleles_frequency_table.txt’ output table was used. The number of modified alleles was determined by filtering > ‘Read_status’ > modified. Average read count per modified allele = modified reads/N modified alleles.
Genotyping of editants
Request a detailed protocolGenotyping was performed on DNA extractions (see above) of three replicates containing five randomly picked editants each of BE4-Gam and heiBE4-Gam injections. Q5 polymerase (NEB), primers fwd 5’-GTTAAAACAGTTTCTTAAAAAGAACAGGA-3’ and rev 5’-AGCAGAAGAAATGACTCAACATTTTG-3’ (annealing at 62°C) were used on 1 µl of diluted DNA sample according to the manufacturer’s instructions with 30× PCR cycles. PCR products were analyzed on a 1% agarose gel, bands excised, DNA extraction performed using innuPREP Gel Extraction Kit (Analytik Jena) according to the manufacturer’s instructions and subjected to Sanger sequencing (see below).
Cell lines
Request a detailed protocolMouse SW10 cells (ATCC, CRL-2766, Lot number 4117643) were cultured in DMEM (Gibco) supplemented with 1 g/ml glucose containing 10% FCS (Sigma), 1% penicillin (10,000 units/ml; Gibco), and 1% streptomycin (10 mg/ml; Gibco) and maintained at 33°C and 5% CO2 and regularly tested negative for mycoplasma infections. Cells were passaged at 80–90% confluency. Twenty-four hr before transfection cells were seeded in a density of 85,000 cells per 12 wells.
CRISPR Transfection crRNA targeting exon 6 (TCGTATCCAGACACCGTCCC[GGG], PAM in brackets) of the mouse Periaxin (MmPrx) gene was selected from the IDT (crRNA XT) predesign crRNA database. crRNA (50 µM) and Alt-R CRISPR-Cas9 tracrRNA, ATTO-550 (50 µM; IDT, 1075927) were diluted in nuclease-free duplex buffer (IDT) to a final concentration of 1 µM and incubated at 95°C for 5 min. One µg of the corresponding Cas9 mRNA (GeneArt CRISPR nuclease Invitrogen, A29378; JDS246-Cas9 or heiCas9) and 15 µl of tracrRNA+crRNA Mix (1 µM) were diluted in 34 µl Opti-MEM I (Gibco) and mixed with 3 µl Lipofectamine RNAiMAX (ThermoFisher) diluted in 47 µl Opti-MEM I. The tracrRNA+crRNA lipofection mix was incubated for 20 min at RT. Cell culture medium was exchanged with 900 µl Opti-MEM I and the tracrRNA+crRNA lipofection mix was added dropwise to the well. After 48 hr, genomic DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen, 69506) following the manufacturer’s protocol. Q5-PCR was carried out using primers flanking the targeted exon 6 (fwd 5’-GAGACACTCACTCCAGACCC-3’; rev 5’-ACTCAGTAACCCAACAGCCA-3’) and 30 cycles. PCR amplicons were purified using the Monarch DNA Gel Extraction Kit (NEB, T1020S) and subjected to sequencing.
Sanger sequencing
Request a detailed protocolSanger sequencing was performed by Eurofins Genomics using fwd 5’-GTTAAAACAGTTTCTTAAAAAGAACAGGA-3’ to evaluate base editing at OlOca2 T1 target site and using fwd 5’-GAGACACTCACTCCAGACCC-3’ and rev 5’-ACTCAGTAACCCAACAGCCA-3’ to evaluate genome editing of the Prx locus in SW10 cells. Quantification of base editing from Sanger sequencing reads was performed with EditR (Kluesner et al., 2018). Genome editing efficiency was assessed by sequence analysis using the TIDE web tool (Brinkman et al., 2014) and by ICE (Hsiau et al., 2018) using default parameters and indel size range up to 30 bp.
Data visualization
Request a detailed protocolData visualization and figure assembly was performed using Fiji (Schindelin et al., 2012), ggplot2 (Wickham, 2016) in RStudio (Team, 2020), Geneious Prime 2019.2.1, Adobe Illustrator CS6 and Affinity Designer 1.10.5.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files.
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Article and author information
Author details
Funding
Deutsche Forschungsgemeinschaft (CRC873 project A3)
- Joachim Wittbrodt
Deutsche Forschungsgemeinschaft (FOR2509 P10 WI 1824/9-1)
- Joachim Wittbrodt
Deutsche Forschungsgemeinschaft (CRC1118 project S03)
- Marc Freichel
H2020 European Research Council (810172)
- Joachim Wittbrodt
Deutsche Forschungsgemeinschaft (3DMM2O, EXC 2082/1 Wittbrodt C3)
- Joachim Wittbrodt
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Acknowledgements
This research was supported by grants of the European Research Council (ERC-SyG H2020, 810172), the Excellence Cluster ‘3D Matter Made to Order’ (3DMM2O, EXC 2082/1 Wittbrodt C3) funded through the German Excellence Strategy via Deutsche Forschungsgemeinschaft (DFG), CRC873, project A3 and FOR2509 project 10 (WI 1824/9-1) to JW and CRC1118, project S03 to MF. AC, BW, RM, and TT-S are members/alumni of HBIGS, the Heidelberg Biosciences International Graduate School. BW was supported by the Deutsches Zentrum für Herz-Kreislauf-Forschung (DZHK B20-024 SE). We thank T Kellner for sgRNA, base editor, and Cas9 mRNA synthesis. We are thankful to M Majewski, E Leist, S Erny and A Saraceno for fish husbandry. We thank all members of the Wittbrodt lab for their critical, constructive feedback on the procedure and the manuscript.
Copyright
© 2022, Thumberger et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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