The E3 ligase Thin controls homeostatic plasticity through neurotransmitter release repression

  1. Martín Baccino-Calace
  2. Katharina Schmidt
  3. Martin Müller  Is a corresponding author
  1. University of Zurich, Switzerland

Abstract

Synaptic proteins and synaptic transmission are under homeostatic control, but the relationship between these two processes remains enigmatic. Here, we systematically investigated the role of E3 ubiquitin ligases, key regulators of protein degradation-mediated proteostasis, in presynaptic homeostatic plasticity (PHP). An electrophysiology-based genetic screen of 157 E3 ligase-encoding genes at the Drosophila neuromuscular junction identified thin, an ortholog of human tripartite motif-containing 32 (TRIM32), a gene implicated in several neurological disorders, including autism spectrum disorder and schizophrenia. We demonstrate that thin functions presynaptically during rapid and sustained PHP. Presynaptic thin negatively regulates neurotransmitter release under baseline conditions by limiting the number of release-ready vesicles, largely independent of gross morphological defects. We provide genetic evidence that thin controls release through dysbindin, a schizophrenia-susceptibility gene required for PHP. Thin and Dysbindin localize in proximity within presynaptic boutons, and Thin degrades Dysbindin in vitro. Thus, the E3 ligase Thin links protein degradation-dependent proteostasis of Dysbindin to homeostatic regulation of neurotransmitter release.

Data availability

All data generated or analyzed during this study are included in the manuscript and supporting files. Source data files have been provided for Figures 1-6.

Article and author information

Author details

  1. Martín Baccino-Calace

    Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
  2. Katharina Schmidt

    Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-2797-9952
  3. Martin Müller

    Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland
    For correspondence
    Martin.Mueller@imls.uzh.ch
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-1624-6761

Funding

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (PP00P3-15)

  • Martin Müller

European Research Council (SynDegrade-679881)

  • Martin Müller

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Copyright

© 2022, Baccino-Calace et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Martín Baccino-Calace
  2. Katharina Schmidt
  3. Martin Müller
(2022)
The E3 ligase Thin controls homeostatic plasticity through neurotransmitter release repression
eLife 11:e71437.
https://doi.org/10.7554/eLife.71437

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https://doi.org/10.7554/eLife.71437