The extensive autofluorescence in ISH of human dorsal root ganglia (DRG) section prevents automated signal detection. Thus, quantitation of ISH data involves manually scoring positive and negative cells. Therefore, we only quantitated the highest intensity signals with the most clear-cut expression patterns. In total we scored 1101 total cells in sections from a 35-year-old female and an 18-year-old male donor. The proportion of positively scored cells for five probes where signal strength, consistency in expression level and frequency of positives allow confidence in the accuracy of scoring and the representative nature of the quantitation were: NEFH, 54%; SCN10A, 58%; OSMR, 29%; TAC1, 25%; and SST, 12%. (A) Bar graphs displaying the 10 pairs of combinations of these five probes (overlap, gray) represents cells expressing both markers in that bar; where overlap is extensive the identity of the second gene has been added above the bar. (B) Bar graph displaying expression of the three probes shown in Figure 2D; NEFH, blue; OSMR, red; TAC1, green; overlap between NEFH and TAC1, cyan; NEFH and OSMR, magenta; OSMR and TAC1, yellow; only about 5% of cells were not positive for any of these three probes. (C) Bar graph displaying expression of the five probes; all populations that make up more than 5% of cells are colored and labeled. Rare combinations include cells positive for NEFH, SCN10A, and OSMR, 3%; NEFH, SCN10A, SST, and OSMR, 2%; SCN10A only, 2%; SST and OSMR, 2%; and SCN10A, TAC1, SST, and OSMR, 1%. None represents 3% of cells that were not positive for expression of these genes but were detected by other probes TRPM8, NTRK2, PIEZO2, and TRPV1 used for hybridization of these sections.