(A) Methylation distribution of SoC-CpGs, matched controls, and array background in pre-gastrulation inner cell mass (ICM) and post-gastrulation embryonic liver, measured in reduced representation bisulfite-seq (RRBS) embryo methylation data from Guo et al., 2014. Data comprises 112,380 CpGs covered at ≥10× in ICM and/or embryonic liver that overlap array background, including 118 SoC-CpGs and 51 matched controls. (B) Methylation distribution of SoC-CpGs, matched controls, and array background in sperm whole-genome bisulfite-seq (WGBS) data from Okae et al., 2014. Data comprises 294,240 CpGs covered at ≥10× including 196 SoC-CpGs and 207 matched controls. (C) Sperm methylation and oocyte germline differentially methylated region (oo-gDMR) status at 196 SoC-CpGs covered at ≥10× in Okae et al. sperm WGBS data. Sperm hypomethylation is defined as methylation ≤25%. oo-gDMRs defined as sperm methylation <25% and oocyte methylation >75% in WGBS analysis by Sanchez-Delgado et al., 2016. (D) Mean methylation at SoC-CpGs and array background measured in n = 233 individuals in the ENID (2 yr) cohort stratified by sperm and oocyte methylation status. Left: Methylation stratified according to sperm hypomethylation status (n = 175 SoC-CpGs hypomethylated in sperm, n = 21 not hypomethylated). Right: As left but with loci hypomethylated in sperm further stratified according to oo-gDMR status (n = 26 SoC-CpGs hypermethylated in oocytes/oo-gDMR, n = 149 not hypermethylated). Sperm/oo-gDMR status thresholds as for Figure 4C. Eight PofOm CpGs (green triangles) are those identified in Zink et al., 2018.