Analysis of rod/cone gap junctions from the reconstruction of mouse photoreceptor terminals
- University of Texas at Houston, United States
- Oregon Health & Science University, United States
- University of Maryland, College Park, United States
- National Eye Institute, National Institutes of Health, United States
Abstract
This project was inspired by the paper from Behrens et al (2016) who used e2006 to reconstruct bipolar cells. We thank Christian Behrens, Timm Schubert, Philipp Berens and Thomas Euler (University of Tübingen) for generously sharing data on blue cone bipolar cells. We thank Moritz Helmstaedter (MPI, Frankfurt) for hosting the e2006 dataset. We thank Kiril Martemyanov (Scripps research Institute, Jupiter, Florida) for the generous gift of an mGluR6 antbody. We thank David Berson (Brown University), for advice, encouragement and an introduction to connectomics. We thank Jessica Riesterer at the Multiscale Microscopy Core, an OHSU University Shared Resource core facility, for acquiring the FIB-SEM datasets. We thank Alice Chuang (Richard Ruiz Department of Ophthalmology and Visual Science, McGovern Medical School) for statistical analysis.
Data availability
All the data used to create the figures in the manuscript have been provided as source data files for Figures 2, 3, 4, 5 and 8.The following data sets were generated.Ishibashi M, Keung J, Ribelayga CP, Massey SC (2018) Confocal imaging of the outer plexiform layer in mouse retina. Collection ID: 30675648bee2309e, URL: https://download.brainimagelibrary.org/30/67/30675648bee2309e/In the public domain at BIL http://www.brainimagelibrary.org/index.htmlSinger JH (2018) SBF-SEM of mouse retina. eel001. URL: https://wklink.org/9712In the public domain at webKnossos https://webknossos.org/Morgan CW, Aicher SA, Carroll JR (2019) FIB-SEM of the outer plexiform layer in light-adapted mouse retina. EM1 and EM2, URL: https://bossdb.org/project/ishibashi2021In the public domain at BossDB https://bossdb.org/
Article and author information
Author details
Munenori Ishibashi
Christophe P Ribelayga
Funding
National Institute of Mental Health (RF1MH127343)
- Catherine W Morgans
- Sue A Aicher
- Christophe P Ribelayga
- Stephen C Massey
National Eye Institute (EY029408)
- Christophe P Ribelayga
- Stephen C Massey
National Eye Institute (EY017836)
- Joshua H Singer
National Institute of Neurological Disorders and Stroke (P30NS061800)
- Sue A Aicher
National Eye Institute (P30EY028102)
- Stephen C Massey
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Fred Rieke, University of Washington, United States
Ethics
Animal experimentation: All animal procedures were reviewed and approved by the Animal Welfare Committee at the University of Texas Health Science Center at Houston (AWC-20-0138) or by our collaborators' local Institutional Animal Care and Use Committees.
Version history
- Received: August 13, 2021
- Preprint posted: September 6, 2021 (view preprint)
- Accepted: April 25, 2022
- Accepted Manuscript published: April 26, 2022 (version 1)
- Accepted Manuscript updated: April 27, 2022 (version 2)
- Version of Record published: June 6, 2022 (version 3)
Copyright
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
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Analysis of rod/cone gap junctions from the reconstruction of mouse photoreceptor terminals
eLife 11:e73039.
https://doi.org/10.7554/eLife.73039
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