Thermosynechococcus switches the direction of phototaxis by a c-di-GMP-dependent process with high spatial resolution
- Department of Engineering Science, Graduate School of Informatics and Engineering, The University of Electro-Communications, Japan
- Institute of Biology III, University of Freiburg, Germany
- Research Core Unit Metabolomics, Hannover Medical School, Germany
- Department of Applied Chemistry and Life Science, Toyohashi University of Technology, Japan
- BIOSS Centre for Biological Signaling Studies, University of Freiburg, Germany
- Department of Physics, Gakushuin University, Japan
Figures
Figure 1
Positive and negative phototaxis of T.
vulcanus. (A) Phototaxis on agar plates. Images were taken 8 hr after spotting the cell suspension. (B) Diagram of the experimental setup to visualize single cellular behaviour under optical …
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Figure 1—source data 1
Rose plots.
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Figure 1—source data 2
Cell displacement.
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Figure 2 with 6 supplements
Wavelength dependency of phototaxis via optical microscopy.
(A) Schematics of the lateral illumination for phototaxis. Five LEDs were simultaneously applied through dichroic mirrors from the right side. (B) Spectra of lateral and vertical light for …
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Figure 2—source data 1
Effects of the light source on the phototactic behaviour of cells on a glass surface.
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Figure 2—source data 2
Rose plots.
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Figure 2—figure supplement 1
Cell movement after applying a monochromatic light source.
(A) Spectra of each LED used for stimulation. No lateral illumination in the dark condition. Infrared light was used for cell observation from a halogen lamp through a bandpass filter (see more …
Figure 2—figure supplement 2
Cell movement at various fluence rates of a lateral green light.
(A) Cell images at 4 min after the lateral light was turned on. (B) Kymograph of cell movements along the optical axis of lateral illumination. Directional movements of cells are presented by the …
Figure 2—figure supplement 3
Phototactic behaviour of wild type (WT) cells at various temperatures.
Lateral light from a single LED was adjusted to a fluence rate of 70 μmol m−2 s−1 and applied from the right side of the image. (A) Cell image at 4 min after the lateral light was turned on. (B) …
Figure 2—figure supplement 4
Cell movement after applying a dichromatic light source.
(A) Spectra of two LEDs used for stimulation. (B) Cell images at 4 min after the lateral light was turned on. Lateral light from two LEDs was adjusted at a fluence rate of 70 μmol m−2 s−1 for each …
Figure 2—figure supplement 5
Dose dependency of blue light to induce negative phototaxis.
Lateral blue light was adjusted at various fluence rates, while lateral green light was fixed at 70 μmol m−2 s−1, as presented at the top. (A) Cell images at 4 min after the lateral light was turned …
Figure 2—figure supplement 6
Effect of blue light illumination from the other side of green light on phototaxis.
(A) Schematic of the lateral light illumination. Blue and green light were applied from the left and right sides of the image at 70 μmol m−2 s−1, respectively. (B) Cell images at 4 min after the …
Figure 3 with 1 supplement
Photoreceptors for phototaxis.
(A) Domain composition of candidate photoreceptor-containing proteins in T. vulcanus. (B) Mutant cell behaviour after lateral light illumination. Upper: lateral green light at a fluence rate of 70 …
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Figure 3—source data 1
Mutant cell behaviour after lateral light illumination.
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Figure 3—source data 2
Rose plots.
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Figure 3—source data 3
Intracellular cyclic dimeric GMP (c-di-GMP) concentrations in wild type (WT) and ∆sesA.
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Figure 3—figure supplement 1
Schematic illustration of cellular cyclic dimeric GMP (c-di-GMP) levels and cell migration within a dense cyanobacterial community under solar irradiation.
Blue light is rich at the surface of such a community (e.g., a phototrophic mat in a hot spring). Synthesis of c-di-GMP induces downward migration at the surface, while degradation of c-di-GMP …
Figure 4
Effect of the tll1859 mutation on phototaxis and intracellular cyclic dimeric GMP (c-di-GMP) concentrations.
(A) Domain composition of Tll1859 protein. The WT_N strain has a frameshift mutation in the gene sequence of the EAL domain. (B) Predicted structure of the Tll1859-EAL domain by SWISS-MODEL. The …
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Figure 4—source data 1
Intracellular cyclic dimeric GMP (c-di-GMP) concentrations in WT_N and tll1859toN mutant.
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Figure 4—source data 2
List of nucleotide differences found in ‘WT_N’ compared with ‘WT’ of T.
vulcanus.
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Figure 5
Type IV pili (T4P) machinery and phototaxis.
(A) Schematic of the T4P machinery. Protein components and their gene IDs from the genome of T. vulcanus are indicated. The location of the components is presented in reference to other species (Chan…
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Figure 5—source data 1
Effect of lateral light illumination on mutant cells.
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Figure 6
Moving trajectories of cells during negative phototaxis.
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Figure 6—source data 1
Moving trajectories.
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Figure 6—source data 2
Area of a cell.
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Figure 6—source data 3
Moving direction of a cell in relation to the light source.
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Figure 7 with 3 supplements
Visualization of type IV pili (T4P) dynamics through nano-beads.
(A) Schematic of the beads’ assay. Sulfate beads (200 nm in diameter) were added to the cells on a glass surface. (B) Typical trajectories of beads. Left: a series of images. Time was presented at …
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Figure 7—source data 1
Moving trajectories of beads in Figure 7BF.
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Figure 7—source data 2
Time course of the distance between the bead and a cell pole.
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Figure 7—source data 3
Distribution of the bead velocity in Figure 7E, Figure 7—figure supplement 2 −.
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Figure 7—source data 4
Rose plot.
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Figure 7—figure supplement 1
Optimization of beads assay.
(A) Type IV pili (T4P)-dependent bead accumulation at the cell pole. Cell images were observed by dark-field microscopy 2 min after adding 200-nm diameter sulfate beads. Yellow indicates bead …
Figure 7—figure supplement 2
Effect of the illumination of green and green blue light on type IV pili (T4P) dynamics.
T4P dynamics were visualized through 200-nm diameter sulfate beads. The velocity of bead movement was measured by the time course of bead displacement. The movement towards the cell was measured as …
Figure 7—figure supplement 3
Micro-optics effect.
(A) Cell image via optical microscopy. Left: cell image visualized by the lateral illumination of green light from the right side of the image. Middle: cell outline visualized by the vertical …
Figure 8
Direct visualization of type IV pili (T4P) dynamics by PilA labelling in living cells.
(A) Schematic of the epi-fluorescent microscopy setup. (B) Distribution of the velocity of T4P dynamics. The velocity of pilus dynamics was measured as the time course of the displacement of the …
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Figure 8—source data 1
Distribution of the velocity of type IV pili (T4P) dynamics in Figure 8B.
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Figure 8—source data 2
Rose plot of the type IV pili (T4P) filaments in Figure 8F.
- https://cdn.elifesciences.org/articles/73405/elife-73405-fig8-data2-v1.xlsx
Videos
Video 1
Positive phototaxis.
Lateral illumination of green light was turned on at time 0 from the right side of the movie. Wild type (WT) cells at 45°C. Area 750 × 563 μm.
Video 2
Microcolony formation.
Lateral illumination of blue light was turned on at time 0 from the right side of the movie. Wild type (WT) cells at 45°C. Area 750 × 563 μm.
Video 3
Phototaxis at low temperature.
Lateral illumination of green light was turned on at time 0 from the right side of the movie. Wild type (WT) cells at 25°C. Area 750 × 563 μm.
Video 4
Negative phototaxis.
Lateral illumination of green and blue light was turned on at time 0 from the right side of the movie. Wild type (WT) cells at 45°C. Area 750 × 563 μm.
Video 5
On–off control of positive phototaxis.
Lateral illumination of green light was applied with 4-min intervals from the right side of the movie. Wild type (WT) cells at 45°C. Area 750 × 563 μm.
Video 6
On–off control of negative phototaxis.
Lateral illumination of green and blue light was applied with 4-min intervals from the right side of the movie. Wild type (WT) cells at 45°C. Area 750 × 563 μm.
Video 7
Directional switch of phototaxis in wild type (WT).
Lateral illumination was turned on at time 0 from the right side of the movie. The illumination was applied with three phases in the order of green, green/blue, and green with a time interval of 4 …
Video 8
Cell behaviour in ΔsesA mutant.
Lateral illumination was turned on at time 0 from the right side of the movie. The illumination was applied with three phases in the order of green, green/blue, and green with a time interval of 4 …
Video 9
Cell behaviour in ΔsesC mutant.
Lateral illumination was turned on at time 0 from the right side of the movie. The illumination was applied with three phases in the order of green, green/blue, and green with a time interval of 4 …
Video 10
Cell behaviour in ΔsesB mutant.
Lateral illumination was turned on at time 0 from the right side of the movie. The illumination was applied with three phases in the order of green, green/blue, and green with a time interval of 4 …
Video 11
Cell behaviour of a ΔpilB mutant.
Lateral illumination of green light was turned on at time 0 from the right side of the movie. ΔpilB cells at 45°C. Area 750 × 563 μm.
Video 12
Phototaxis of microcolony.
Lateral illumination of green and blue light was applied from the right side of the movie. Wild type (WT) cells at 45°C. Area 117 × 88 μm.
Video 13
Phototaxis of cell perpendicular to the lateral light axis.
Lateral illumination of green and blue light was applied from the right side of the movie. Wild type (WT) cell at 45°C. Area 171 × 44 μm.
Video 14
Phototaxis of cell that stood up and kept binding at a cell pole.
Lateral illumination of green and blue light was applied from the right side of the movie. Wild type (WT) cell at 45°C. Area 171 × 44 μm.
Video 15
Type IV pili (T4P) dynamics through beads.
Extension and retraction of the T4P filament were visualized by sulfate beads with a size of 200 nm. Wild type (WT) cells at 45°C. Area 42.0 × 31.5 μm.
Video 16
Type IV pili (T4P) dynamics through beads during negative phototaxis.
Lateral illumination was applied from the right side of the movie. Extension and retraction of the T4P filament were visualized by beads with a size of 200 nm. Dark-field microscopy. Wild type (WT) …
Video 17
Type IV pili (T4P) dynamics under epi-fluorescence microscopy.
Extension and retraction of the T4P filaments were visualized by fluorescence labelling of PilA1 at 45°C. Area 39.0 × 29.3 μm.
Video 18
Type IV pili (T4P) dynamics during directional movement under epi-fluorescence microscopy.
Extension and retraction of the T4P filaments were visualized by fluorescence labelling of PilA1. The cell showed directional movement towards the left side at 45°C. Area 26.0 × 19.5 μm.
Video 19
Type IV pili (T4P) dynamics under total internal reflection fluorescence (TIRF) microscopy.
Surface attachment of the T4P filaments was visualized by fluorescence labelling of PilA1 under TIRF illumination at 45°C. Area 39.0 × 29.3 μm.
Video 20
Type IV pili (T4P) dynamics during directional movement under total internal reflection fluorescence (TIRF) microscopy.
Surface attachment of the T4P filaments was visualized by fluorescence labelling of PilA1 under TIRF illumination. The cell showed directional movement towards the left side at 45°C. Area 26.0 × …
Tables
Table 1
Oligonucleotides used in this study.
| Name | Sequence (5′–3′) | Purpose |
|---|---|---|
| pUC19-5R_tvsesA | ATGATACGCGGGTGATTACCGAGCTCGAATTCAC | ∆sesA |
| pUC19-6F_tvsesA | GTGGCAAAACCACCTTTGCAAGCTTGGCGTAATC | |
| tvsesA-1F_pUC19 | GAATTCGAGCTCGGTAATCACCCGCGTATCATTG | |
| tvsesA-2R_Cm | TTCTATCAGCTGTCCCATCAATCCCCGAAACTGC | |
| Cm-3F_tvsesA | AGTTTCGGGGATTGATGGGACAGCTGATAGAAAC | |
| Cm-4R_tvsesA | ATGGGTGATATTGGCTTTTATCAGGCTCTGGGAG | |
| tvsesA-3F_Cm | CCCAGAGCCTGATAAAAGCCAATATCACCCATGC | |
| tvsesA-4R_pUC19 | TTACGCCAAGCTTGCAAAGGTGGTTTTGCCACAG | |
| pUC19-7R_tvsesB | TCAGGGGAGTCCAAAATACCGAGCTCGAATTCAC | ∆sesB |
| pUC19-8F_tvsesB | ACGGATTGCCATTGGTTGCAAGCTTGGCGTAATC | |
| tvsesB-1F_pUC19 | GAATTCGAGCTCGGTATTTTGGACTCCCCTGATG | |
| tvsesB-2R_Km | AGCAGGGGAATTGTTACACAGACTCTTCTGTGAC | |
| Km-5F_tvsesB | CACAGAAGAGTCTGTGTAACAATTCCCCTGCTCG | |
| Km-6R_tvsesB | GTTCAAGGTCCTCTTGCCAACCTTTCATAGAAGGC | |
| tvsesB-3F_Km | TTCTATGAAAGGTTGGCAAGAGGACCTTGAACTG | |
| tvsesB-4R_pUC19 | TTACGCCAAGCTTGCAACCAATGGCAATCCGTAG | |
| pUC19-21R_tvcikA | AGATGCGATTGTCCATTACCGAGCTCGAATTCAC | ∆cikA |
| pUC19-22F_tvcikA | TTCATTGGGCTTACAGTGCAAGCTTGGCGTAATC | |
| tvcikA-1F_pUC19 | GAATTCGAGCTCGGTAATGGACAATCGCATCTGC | |
| tvcikA-2R_Cm | TTCTATCAGCTGTCCCGCTGATAGCTACTGCTAG | |
| Cm-18F_tvcikA | AGCAGTAGCTATCAGCGGGACAGCTGATAGAAAC | |
| Cm-19R_tvcikA | AGATATTCCCGCTGTTTTTATCAGGCTCTGGGAG | |
| tvcikA-3F_Cm | CCCAGAGCCTGATAAAAACAGCGGGAATATCTGG | |
| tvcikA-4R_pUC19 | TTACGCCAAGCTTGCACTGTAAGCCCAATGAACC | |
| pUC19-19R_tvpixD | GTTACCATTCGCAAGTTACCGAGCTCGAATTCAC | ∆pixD |
| pUC19-20F_tvpixD | GCTAGTCCTGAACCAATGCAAGCTTGGCGTAATC | |
| tvpixD-1F_pUC19 | GAATTCGAGCTCGGTAACTTGCGAATGGTAACCG | |
| tvpixD-2R_Cm | TTCTATCAGCTGTCCCAATCAGGCGATGTAGTCC | |
| Cm-15F_tvpixD | ACTACATCGCCTGATTGGGACAGCTGATAGAAAC | |
| Cm-16R_tvpixD | CAGCATTCCCGTAATGTTTATCAGGCTCTGGGAG | |
| tvpixD-3F_Cm | CCCAGAGCCTGATAAACATTACGGGAATGCTGTG | |
| tvpixD-4R_pUC19 | TTACGCCAAGCTTGCATTGGTTCAGGACTAGCTG | |
| pUC19-23R_tvtll1282 | TTTGGCATCTTCAAGGTACCGAGCTCGAATTCAC | ∆LOV |
| pUC19-24F_tvtll1282 | TGAGCAAAGGGATCATTGCAAGCTTGGCGTAATC | |
| tvtll1282-1F_pUC19 | GAATTCGAGCTCGGTACCTTGAAGATGCCAAAGC | |
| tvtll1282-2R_Cm | TTCTATCAGCTGTCCCAGCATAGCGTTCCATCTC | |
| Cm-20F_tvtll1282 | GATGGAACGCTATGCTGGGACAGCTGATAGAAAC | |
| Cm-21R_tvtll1282 | AATAGGCTCAGGACATTTTATCAGGCTCTGGGAG | |
| tvtll1282-3F_Cm | CCCAGAGCCTGATAAAATGTCCTGAGCCTATTGG | |
| tvtll1282-4R_pUC19 | TTACGCCAAGCTTGCAATGATCCCTTTGCTCAGC | |
| pUC19-25R_tvHCP | TGAACTTTGCCCTTGTTACCGAGCTCGAATTCAC | ∆OCP |
| pUC19-26F_tvCTDH | GATAGGGAGATTGCATTGCAAGCTTGGCGTAATC | |
| tvHCP-1F_pUC19 | GAATTCGAGCTCGGTAACAAGGGCAAAGTTCAGC | |
| tvHCP-2R_Cm | TTCTATCAGCTGTCCCGCTCATCAAAACTGAGGG | |
| Cm-22F_tvHCP | CTCAGTTTTGATGAGCGGGACAGCTGATAGAAAC | |
| Cm-23R_tvCTDH | TCTTCAAAGGGTGGATTTTATCAGGCTCTGGGAG | |
| tvCTDH-1F_Cm | CCCAGAGCCTGATAAAATCCACCCTTTGAAGAGC | |
| tvCTDH-2R_pUC19 | TTACGCCAAGCTTGCAATGCAATCTCCCTATCCG | |
| pUC19-15R_tvpilB | CAGTTGAATCTGGGTTTACCGAGCTCGAATTCAC | ∆pilB |
| pUC19-16F_tvpilB | ACAGGTTTTGGAGGTCTGCAAGCTTGGCGTAATC | |
| tvpilB-1F_pUC19 | GAATTCGAGCTCGGTAAACCCAGATTCAACTGGG | |
| tvpilB-2R_Cm | TTCTATCAGCTGTCCCTTGTGGTACTGGCGTAAC | |
| Cm-11F_tvpilB | TACGCCAGTACCACAAGGGACAGCTGATAGAAAC | |
| Cm-12R_tvpilB | AGATCTTGAAGCGGGATTTATCAGGCTCTGGGAG | |
| tvpilB-3F_Cm | CCCAGAGCCTGATAAATCCCGCTTCAAGATCTTG | |
| tvpilB-4R_pUC19 | TTACGCCAAGCTTGCAGACCTCCAAAACCTGTAC | |
| pUC19-17R_tvpilT1 | TTTTTGCCAGCACCTTTACCGAGCTCGAATTCAC | ∆pilT1 |
| pUC19-18F_tvpilT1 | TCTTGGCTGGCTTGATTGCAAGCTTGGCGTAATC | |
| tvpilT1-1F_pUC19 | GAATTCGAGCTCGGTAAAGGTGCTGGCAAAAAGC | |
| tvpilT1-2R_Cm | TTCTATCAGCTGTCCCAAGTCTGAACCACCGTTG | |
| Cm-13F_tvpilT1 | ACGGTGGTTCAGACTTGGGACAGCTGATAGAAAC | |
| Cm-14R_tvpilT1 | TGGCAAGCTGAATCGTTTTATCAGGCTCTGGGAG | |
| tvpilT1-3F_Cm | CCCAGAGCCTGATAAAACGATTCAGCTTGCCATC | |
| tvpilT1-4R_pUC19 | TTACGCCAAGCTTGCAATCAAGCCAGCCAAGAAG | |
| pUC19-11R_tvpilA1 | CTATTCTCTTTGCAGGTACCGAGCTCGAATTCAC | ∆pilA1 |
| pUC19-12F_tvpilA1 | TGGTTTGGTGCCAGTATGCAAGCTTGGCGTAATC | |
| tvpilA1-1F_pUC19 | GAATTCGAGCTCGGTACCTGCAAAGAGAATAGCG | |
| tvpilA1-7R_Cm | TTCTATCAGCTGTCCCAGTTGCCGTCTGCTACAG | |
| Cm-32F_tvpilA1 | GTAGCAGACGGCAACTGGGACAGCTGATAGAAAC | |
| Cm-6R_tvpilA1 | GCAGTTGCACTATTGCTTTATCAGGCTCTGGGAG | |
| tvpilA1-3F_Cm | CCCAGAGCCTGATAAAGCAATAGTGCAACTGCTC | |
| tvpilA1-4R_pUC19 | TTACGCCAAGCTTGCATACTGGCACCAAACCATG | |
| pUC19-13R_tvHfq | CAGCGGGATGTGAATATACCGAGCTCGAATTCAC | ∆hfq |
| pUC19-14F_tvHfq | AAATGCGGCTTTCCTATGCAAGCTTGGCGTAATC | |
| tvHfq-1F_pUC19 | GAATTCGAGCTCGGTATATTCACATCCCGCTGTG | |
| tvHfq-2R_Cm | TTCTATCAGCTGTCCCTACTTGGCGAATGCTAGG | |
| Cm-9F_tvHfq | TAGCATTCGCCAAGTAGGGACAGCTGATAGAAAC | |
| Cm-10R_tvHfq | ACCTCGGTGCTATCTTTTTATCAGGCTCTGGGAG | |
| tvHfq-3F_Cm | CCCAGAGCCTGATAAAAAGATAGCACCGAGGTTG | |
| tvHfq-4R_pUC19 | TTACGCCAAGCTTGCATAGGAAAGCCGCATTTGC | |
| pUC19-43R_tvtll1859 | ATCCATCGAACAAGCCTACCGAGCTCGAATTCAC | tll1859toN |
| pUC19-44F_tvtll1859 | GTCCCGTTCATTGATGTGCAAGCTTGGCGTAATC | |
| tvtll1859-1F_pUC19 | GAATTCGAGCTCGGTAGGCTTGTTCGATGGATTG | |
| tvtll1859-2R_Kmv2 | cctgagtgcttgcggcTTTCTAGGGGGTGTGAATC | |
| Km-21F_tvtll1859 | TCACACCCCCTAGAAAgccgcaagcactcagg | |
| Km-22R_tvtll1859 | TTGCAACACAGTTGTCcctttcatagaaggcggc | |
| tvtll1859-3F_Kmv2 | cgccttctatgaaaggGACAACTGTGTTGCAAACC | |
| tvtll1859-4R_pUC19 | TTACGCCAAGCTTGCACATCAATGAACGGGACTG | |
| pUC19-67F_tvpilA1 | gattggtttcaggcaaTGCAAGCTTGGCGTAATC | PilA1S114C |
| pUC19-68R_tvPilA1 | aacgatagggagacatTACCGAGCTCGAATTCAC | |
| tvPilA1-8F_pUC19 | GAATTCGAGCTCGGTAatgtctccctatcgttctag | |
| TvPilA1-9R_CmR | TTCTATCAGCTGTCCCttaagaacagttaggagcag | |
| Cm-44F_TvPilA1 | tcctaactgttcttaaGGGACAGCTGATAGAAAC | |
| Cm-45R_TvPilA1 | gttaattcctaaggtcTTTATCAGGCTCTGGGAG | |
| TvPilA1-10F_Cm | CCCAGAGCCTGATAAAgaccttaggaattaacagag | |
| TvPilA1-11R_pUC19 | TTACGCCAAGCTTGCAttgcctgaaaccaatcgg | |
| tvPilA1(S114C)–1F | caaacaTGCgctaatgatccccaaggg | |
| tvPilA1(S114C)–2R | attagcGCAtgtttgaacataagcatcattc |
Table 2
Plasmids used in this study.
| Name | Description | Reference/source |
|---|---|---|
| pUC19-∆tvsesA_Cm | pUC19-based construct for knockout of sesA gene (NIES2134_109940) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of sesA, resulting in ~2000 bp deletion within ORF; CmR, AmpR | This study |
| pUC19-∆tvsesB_Km | pUC19-based construct for knockout of sesB gene (NIES2134_119260) containing a kanamycin resistance cassette flanked by ~2500 bp regions upstream and downstream of sesB, resulting in ~2000 bp deletion within ORF; KmR, AmpR | This study |
| pS-∆tlr0911_Sp(F) | pCR-Script-based construct for knockout of sesC gene (NIES2134_110090) containing a spectinomycin/streptomycin resistance cassette flanked by ~1500 bp regions upstream and downstream of sesC, resulting in ~3400 bp deletion within ORF; SpR/SmR, AmpR | Enomoto et al., 2015 |
| pUC19-∆tvcikA_Cm | pUC19-based construct for knockout of cikA gene (NIES2134_110210) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of cikA, resulting in ~500 bp deletion within ORF; CmR, AmpR | This study |
| pUC19-∆tvpixD_Cm | pUC19-based construct for knockout of pixD gene (NIES2134_124540) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of pixD, resulting in ~100 bp deletion within ORF; CmR, AmpR | This study |
| pUC19-∆tvtll1282_Cm | pUC19-based construct for knockout of LOV gene (tll1282; NIES2134_112750) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of cikA, resulting in ~2000 bp deletion within ORF; CmR, AmpR | This study |
| pUC19-∆tvHCPCTDH_Cm | pUC19-based construct for knockout of OCP genes (HCP; NIES2134_112880 and CTDH; NIES2134_112890) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of OCP, resulting in the deletion of the latter half of HCP ORF, the intergenic region, and the first half of CTDH ORF; CmR, AmpR | This study |
| pUC19-∆tvpilB_Cm | pUC19-based construct for knockout of pilB gene (NIES2134_124120) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of pilB, resulting in ~600 bp deletion within ORF; CmR, AmpR | This study |
| pUC19-∆tvpilT1_Cm | pUC19-based construct for knockout of pilT1 gene (NIES2134_124130) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of pilT1, resulting in ~600 bp deletion within ORF; CmR, AmpR | This study |
| pUC19-∆tvpilA1_Cm | pUC19-based construct for knockout of pilA1 gene (NIES2134_108920) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of pilA1, resulting in ~550 bp deletion within ORF and the promoter region; CmR, AmpR | This study |
| pUC19-∆tvHfq_Cm | pUC19-based construct for knockout of hfq gene (NIES2134_124130) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of hfq, resulting in ~10 bp deletion within ORF; CmR, AmpR | This study |
| pUC19-tvtll1859toN_Kmv2 | pUC19-based construct for introduction of the frameshift mutation present in WT_N within tll1859 gene (NIES2134_117830) containing a kanamycin resistance cassette inserted after tll1859 ORF, flanked by ~2500 bp regions upstream and downstream of tll1859 gene without any nucleotide deletion; KmR, AmpR | This study |
| pUC19-tvpilA1(S114C)_Cm | pUC19-based construct for Cys knockin in pilA1 gene (NIES2134_108920) containing a chloramphenicol resistance cassette inserted after pilA1 ORF, flanked by ~2500 bp regions upstream and downstream of pilA1 without any nucleotide deletion; CmR, AmpR | This study |
Table 3
Strains used in this study.
| Name | Description | Reference/source |
|---|---|---|
| Thermosynechococcus vulcanus WT | Isolated as wild type showing positive phototaxis towards moderate white light from NIES-2134 | Whole genome: AP018202 |
| Thermosynechococcus vulcanus WT_N | Isolated showing negative phototaxis towards moderate white light from NIES-2134 | Seq reads in DDBJ: DRA013349 |
| ∆sesA | sesA knockout mutant (CmR) | This study |
| ∆sesB | sesB knockout mutant (KmR) | This study |
| ∆sesC | sesC knockout mutant (SpR/SmR) | Enomoto et al., 2015 |
| ∆cikA | cikA knockout mutant (CmR) | This study |
| ∆pixD | pixD knockout mutant (CmR) | This study |
| ∆LOV | LOV knockout mutant (CmR) | This study |
| ∆OCP | OCP knockout mutant (CmR) | This study |
| ∆pilB | pilB knockout mutant (CmR) | This study |
| ∆pilT1 | pilT1 knockout mutant (CmR) | This study |
| ∆pilA1 | pilA1 knockout mutant (CmR) | This study |
| ∆hfq | hfq knockout mutant (CmR) | This study |
| tll1859toN | tll1859 frameshift mutant (KmR) | This study |
| PilA1S114C | pilA1 Cys knockin mutant (CmR) | This study |
