Supplementary file 1A-E.
(A) Measurements included in modeling Pace of Aging in the Dunedin Study Birth Cohort. We measured Pace of Aging from repeated assessments of a panel of 19 biomarkers: Body mass index (BMI), Waist-hip ratio, Glycated hemoglobin, Leptin, Blood pressure (mean arterial pressure), Cardiorespiratory fitness (VO2Max), Forced vital capacity ratio (FEV1/FVC), Forced expiratory volume in one second (FEV1), Total cholesterol, Triglycerides, High density lipoprotein (HDL), Lipoprotein(a), Apolipoprotein B100/A1 ratio, estimated Glomerular Filtration Rate (eGFR), Blood Urea Nitrogen (BUN), High Sensitivity C-reactive Protein (hs-CRP), White blood cell count, mean periodontal attachment loss (AL), and the number of dental-caries-affected tooth surfaces (tooth decay). This list includes two new biomarkers, leptin and caries, not included in the original Pace of Aging (Belsky et al., 2015), both of which have now been assessed at multiple waves, allowing growth curve modeling. Telomere length was dropped because of an emerging and yet-unresolved field-wide debate about its measurement (Nettle et al., 2021). All other biomarkers were the same. The 20 year Pace of Aging measure is described in detail elsewhere (Elliott et al., 2021b). (B) CpGs included in the DunedinPACE DNA methylation algorithm, their mean values in the Dunedin Study, and the coefficients for their weights in the DunedinPACE algorithm. (C) Independent associations of DunedinPACE with mortality, morbidity, and disability in models including covariate adjustment for DNA methylation clocks. The table reports effect-sizes for DunedinPACE and DNA methylation clocks from time-to-event analysis of mortality, cardiovascular disease (CVD), and stroke or transient ischemic attack (TIA) and repeated-measures analysis of incident limitations to activities of daily living (ADLs) in the Framingham Heart Study Offspring cohort. Each model includes DunedinPACE, one of the DNA methylation clocks, and covariates for age and sex. Time-to-event model effect-sizes are reported as hazard ratios (HR). Repeated-measures model effect-sizes are reported as incidence-rate ratios (IRR). (D) Physical and cognitive functioning and subjective signs of aging measures in the Dunedin Study. (E) Items included in Nagi, Katz, and Rosow-Breslau scales of limitations to Activities of Daily Living (ADLs).