(A) Detection of mRNA expression of genes involved in iron uptake and export pathways in bone marrow monocytes (BMM), mononuclear pre-osteoclasts (pOC), and mature osteoclasts (OC) by real-time …
Tfr1 is a major transferrin transporter and regulates cellular iron homeostasis in osteoclasts.
C-I to C-V: mitochondrial respiration chain complex-I to complex-V; DMT1: divalent metal ion transporter 1; GLUT1: glucose transporter 1; ROS: reactive oxygen species; Slc1a5: glutamine transporter; …
(A) Representative µCT images of distal femurs of 10-week-old female control (con) and Tfr1ΔLysM mice. scale bar = 1 mm. (B– F) µCT analysis of trabecular and cortical bone mass and structure of …
Tfr1 myeloid conditional knockout female mice develop normally and display increased trabecular bone mass in femurs at 10-week-old and 6-month-old ages.
μCT analysis of distal femurs of three different genotypes of 10-week-old male and female control mice. Tb: trabecular bone; BV/TV: bone volume/tissue volume; Tb.N: trabecular number; Tb.Th: …
The trabecular bone mass and structure among three different genotypes of control mice are similar.
µCT analysis of trabecular bone mass and structure of distal femurs from 10-week-old C57BL6/J background mice. Tb: trabecular bone; BV/TV: bone volume/tissue volume; Tb.N: trabecular number; Tb.Th: …
Partial deletion of Tfr1 by one-allele of LysM-Cre has no effects on bone mass and structure in male and female mice on C57BL6/J background.
The serum total iron concentration of 10-week-old control (con) and Tfr1ΔLysM mice was measured by a colorimetric based iron detection kit from Fisherscientic Inc. The serum hepcidin level was …
Loss of Tfr1 in myeloid osteoclast precursor cells did not alter the serum iron and hepcidin levels and had no effects on erythropoiesis in 10-week-old male and female mice.
(A) Representative µCT images of distal femurs of 10-week-old male control (con) and Tfr1ΔLysM mice. scale bar = 1 mm. (B–F) µCT analysis of trabecular and cortical bone mass and structure of distal …
Tfr1 myeloid conditional knockout male mice develop normally and exhibit no significant changes in trabecular and cortical bone.
µCT analysis of lumber vertebrae of 10-week-old and 6-month-old control (con) and Tfr1ΔLysM mice. Tb: trabecular bone; BV/TV: bone volume/tissue volume; Tb.N: trabecular number; Tb.Th: trabecular …
Loss of Tfr1 in myeloid osteoclast precursor cells had less effects on trabecular bone mass in lumber vertebrae than femurs.
(A) The representative µCT images of distal femurs of male and female control (con) and Tfr1ΔCTSK mice on the 129×C57BL6J mixed background. scale bar = 1 mm. (B–F) µCT analysis of trabecular and …
Deletion of Tfr1 in cathepsin K-Cre expressing osteoclasts results in increased trabecular bone mass in distal femurs of 10-week-old male and female mice.
(A) Representative µCT images of L4 lumber vertebra of male and female control (con) and conditional knockout (Tfr1ΔCTSK) mice on the 129×C57BL6J mixed background. scale bar = 1 mm. (B–E) µCT …
Deletion of Tfr1 in cathepsin K-Cre expressing osteoclasts slightly increases trabecular thickness of lumber vertebrae of 10-week-old male and female mice.
Quantitative PCR detection of the mRNA levels of Tfrc, Fpn, and mt-Co1 in vehicle (v), estrogen (E2), and androgen (T) treated control (con) and Tfr1ΔLysM osteoclast lineage cells. The data are …
Estrogen but not androgen synergistically with Tfr1-deficiency inhibits the mRNA expression of mitochondrial cytochrome c oxidase subunit I in osteoclast lineage cells.
The data are presented as mean ± SD. n=3. ** p<0.01 and *** p<0.001 vs control (con) or vertebra analyzed by one-way ANOVA.
The deletion efficiency of Tfr1 by LysM-Cre is more potent in the distal femur than in the lumber vertebra as examined by quantitative PCR.
(A) Images of fast green and TRAP staining (scale bar = 200 μm) and (B–G) histomorphometric analysis of the metaphysis of decalcified distal femur histological sections of 10-week-old female control …
Loss of Tfr1 in myeloid osteoclast precursors has no impacts on osteoclast number and bone formation in 10-week-old female mice.
(A) Images of fast green and TRAP staining (scale bar = 200 μm) and (B–G) histomorphometric analysis of the metaphysis of decalcified distal femur histological sections of 10-week-old male control …
Loss of Tfr1 in myeloid osteoclast precursors has no impacts on osteoclast number and bone formation in 10-week-old male mice.
Quantitative PCR detect of the mRNA expression of Acp5 and Runx2 in the distal femurs of control (con) and Tfr1ΔLysM 10-week-old female mice. The data are presented as mean ± SD. n=3. * p<0.05 and …
The mRNA levels of osteoclast marker gene Acp5 and osteoblast marker gene Runx2 increase in the distal femurs of female Tfr1ΔLysM mice.
(A) The low (12.5×) and high (100×) magnification of images of TRAP staining of histological sections of decalcified distal femurs of 10-week-old control (con) and Tfr1 conditional knockout (Tfr1ΔCTS…
Deletion of Tfr1 in cathepsin K-Cre expressing osteoclasts increases trabecular bone mass without influence on osteoclastogenesis in vivo.
(A) TRAP staining (scale bar = 10 μm) and quantification of numbers of total and spreading osteoclasts (OCs) cultured on plastic 24-well dishes. n=6. (B) Quantitative PCR detection of the mRNA …
Deletion of Tfr1 in osteoclast myeloid precursors has no effects on osteoclast differentiation but attenuates cytoskeleton organization and bone resorption in mature osteoclasts cultured from female mice.
Immunoblotting for Figure 4C.
(A) TRAP staining (scale bar = 10 μm) and quantification of numbers of total and spreading osteoclasts (OCs) cultured on plastic 24-well dishes. n=6. (B) The protein level of Tfr1 and osteoclast …
Deletion of Tfr1 in osteoclast myeloid precursors has no effects on osteoclast differentiation but attenuates cytoskeleton organization and bone resorption in mature osteoclasts cultured from male mice.
Immunoblotting for Figure 4—figure supplement 1B.
(A) and (B) TRAP staining and quantification of the number of spreading osteoclasts in control (con) and Tfr1ΔLysM osteoclast cultures. n=6. scale bar = 10 μm. (C) and (D) Staining of actin …
High dose of hemin but not ferric ammonium citrate rescues the phenotypes of Tfr1-deficient osteoclasts.
(A) Heatmaps of proteins that are differentially regulated in control (con) and Tfr1ΔLysM bone marrow monocytes (BMM), mononuclear pre-osteoclasts (pOC), and mature osteoclasts (OC) identified by …
Immunoblotting for Figure 6D.
Each band in immunoblots was quantified by densitometry using the NIH ImageJ software. n=3 from three immunoblots. * p<0.05 and *** p<0.001 vs the corresponding vehicle-treated cells of the same …
Quantification of immunoblotting bands in Figure 6D.
(A) Quantification of mitochondrial mass by Mito Tracker green staining in control (con) and Tfr1ΔLysM bone marrow monocytes (BMM), mononuclear pre-osteoclasts (pOC), and mature osteoclasts (OC). (B)…
Loss of Tfr1 inhibits mitochondrial mass, reactive oxygen species (ROS) production, and membrane potential in mature osteoclasts.
(A) A graphic illustration of oxygen consumption measured by a Seahorse Extracellular Flux analyzer. FCCP: carbonyl cyanide p-trifluoro-methoxyphenyl hydrazone, a synthetic mitochondrial uncoupler. …
Tfr1-deletion in osteoclast lineage cells impairs mitochondrial and non-mitochondrial respirations in mature osteoclasts.
(A) A schematic map of cytoskeletal pathways generated by Ingenuity pathway analysis (IPA). The downregulated proteins are shown in green and upregulated proteins are marked in red orange. (B) …
Loss of Tfr1 in osteoclasts inhibits the macrophage colony-stimulating factor (M-CSF) stimulated activation of c-Src and Rac1 and overexpression of Hem1 partially recuses the cytoskeletal organization defect in Tfr1-null osteoclasts.
Immunoblotting for Figure 9B.
Each band in immunoblots was quantified by densitometry using the NIH ImageJ software. n=3 from three immunoblots. * p<0.05, ** p<0.01, and p<0.001 vs control (con) of the same time point; The data …
Quantification of immunoblotting bands in Figure 9B.
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain and strain background (Mus musculus) | Tfrc-flox | Dr. Nancy C Andrews | 129Sv | |
Strain and strain background (Mus musculus) | Tfrc-flox | Zhao Lab | C57BL6/J | |
Strain and strain background (Mus musculus) | Lyz2-Cre | The Jackson Laboratory | cat#004781 | C57BL6/J |
Strain and strain background (Mus musculus) | Ctsk-Cre | Dr. Takashi Nakamura | C57BL6/J | |
Other | alpha-MEM | MilliporeSigma | M0644 | cell culture medium |
Other | Blasticidin | MilliporeSigma | 203,350 | Antibiotics (2 μg/ml) |
Other | DMEM | MilliporeSigma | D-5648 | cell culture medium |
Other | Fetal bovine serum (FBS) | Hyclone | cell culture (10%) | |
Other | 10× penicillin-streptomycin- l-glutamine | MilliporeSigma | G1146 | Antibiotics (1×) |
Other | 10× trypsin/EDTA | Thermo-Fisher Scientific | 15400–054 | Antibiotics (1×) |
Chemical compound and drug | Mouse apo-transferrin | MilliporeSigma | T0523 | |
Chemical compound and drug | Dihydrotestosterone | MilliporeSigma | D-073 | 10–8 M |
Chemical compound and drug | Estrodiol | MilliporeSigma | E2257 | 10–8 M |
Chemical compound and drug | ferric ammonium citrate (FAC) | MilliporeSigma | F5879 | |
Chemical compound and drug | Fe59 ferric chloride | PerkinElmer Inc | ||
Chemical compound and drug | Hemin | MilliporeSigma | H9039 | |
Chemical compound and drug | Sodium bicarbonate | MilliporeSigma | S5761 | |
Other | Albumin | MilliporeSigma | A7906 | Blocking reagent (0.20% in PBS) |
Other | Alexa Fluro-488 Phalloidin | Thermo-Fisher Scientific | A12379 | Filament actin staining (1:400) |
Other | Glycerol | MilliporeSigma | G5516 | mounting reagent (80% in PBS) |
Other | Hoechst 33,342 | Thermo-Fisher Scientific | H3570 | Nuclei staining (1:4,000 in PBS) |
Other | Paraformaldehyde | MilliporeSigma | P6148 | immunofluorescent staining (4% in PBS) |
Other | Triton X-100 | MilliporeSigma | T-9284 | immunofluorescent staining (0.1%) |
Other | NaK tartrate | MilliporeSigma | S6170 | TRAP staining |
Other | Naphthol AS-BI (phosphoric acid solution) | MilliporeSigma | 1,802 | TRAP staining |
Other | 3,3’-diaminobenzidine (DAB) tablets | MilliporeSigma | D-5905 | pit staining |
Other | 30% H2O2 | MilliporeSigma | 216,763 | pit staining |
Other | Peroxidase-conjugated WGA (wheat germ agglutinin) lectin | MilliporeSigma | L-7017 | pit staining (20 μg/ml) |
Other | cOmplete EDTA-free protease inhibitor cocktail | MilliporeSigma | 4693159001 | protease inhibitor |
Other | Enhanced chemiluminescent detection reagents (ECL) | MilliporeSigma | WBKLS0100 | immunoblotting |
Other | Polyvinylidene difluoride membrane (PVDF) | MilliporeSigma | IPVH00010 | immunoblotting |
Other | RIPA buffer | MilliporeSigma | R-0278 | cell lysate |
Antibody | Mouse monoclonal anti-cathepsin K (clone 182–12 G5) | MilliporeSigma | MAB3324 | 1:2000 |
Antibody | Mouse monoclonal anti-HA.11 (clone 16B12) | Biolegend | 901,513 | 1:5000 |
Antibody | Mouse monoclonal anti-Tfr1 (clone H68.4) | Thermo-Fisher Scientific | 13–6800 | 1:500 |
Antibody | Mouse monoclonal anti-tubulin (clone DM1A) | MilliporeSigma | T9026 | 1:3000 |
Antibody | Mouse monoclonal Total OXPHOS Antibody Cocktail | Abcam | ab110413 | 1:1000 |
Antibody | Goat polyclonal HRP-anti-mouse secondary antibody | Cell Signaling Technology | 7,076 | 1:5000 |
Antibody | Goat polyclonal HRP-anti-rabbit secondary antibody | Cell Signaling Technology | 7,074 | 1:5000 |
Commercial assay or kit | High-capacity cDNA reverse transcription kit | Thermo-Fisher Scientific | 4368813 | |
Commercial assay or kit | RNeasy mini kit | Qiagen | 74,104 | |
Commercial assay or kit | Serum TRAcP-5b | Immunodiagnostic Systems | SB-TR103 | |
Commercial assay or kit | RatLaps (CTx-I) EIA | Immunodiagnostic Systems | AC-06F1 | |
Commercial assay or kit | Rat/mouse PINP EIA | Immunodiagnostic Systems | AC-33F1 | |
Commercial assay or kit | Hepcidin Murine-Complete ELISA Kit | Intrinsic Lifesciences | HMC-001 | |
Commercial assay or kit | Pointe Scientific Iron/TIBC Reagents | Fisherscientific | 23-666-320 | |
Commercial assay or kit | Iron Assay Kit | Abcam | ab83366 | |
Commercial assay or kit | Active Arf1 pull-down and detection kit | Thermo-Fisher Scientific | 16,121 | |
Commercial assay or kit | Active Rac1 pull-down and detection kit | Thermo-Fisher Scientific | 16,118 | |
Commercial assay or kit | TMTsixplex isobaric Mass Tagging kit | Thermo-Fisher Scientific | 90,064 | |
Other | MitoTracker Green fluorescence | Thermo-Fisher Scientific | M7514 | Mitochondrial assay reagent |
Other | MitoSOX Red | Thermo-Fisher Scientific | M36008 | Mitochondrial assay reagent |
Other | JC-1 Dye | Thermo-Fisher Scientific | T3168 | Mitochondrial assay reagent |
Recombinant DNA reagent | Murine Nckap1l | Dharmacon Inc | MMM1013-202769283 | cDNA template |
Commercial assay or kit | TransIT-LT1 | Mirus Bio LLC | MIR2300 | DNA transfection reagent |
Commercial assay or kit | Acp5 | Thermo-Fisher Scientific | Mm00475698_m1 | Quantitative PCR (qPCR) primer |
Commercial assay or kit | Ctsk | Thermo-Fisher Scientific | Mm00484039_m1 | qPCR primer |
Commercial assay or kit | mt-Cox1 | Thermo-Fisher Scientific | Mm00432648_m1 | qPCR primer |
Commercial assay or kit | Dcstamp | Thermo-Fisher Scientific | Mm04209236_m1 | qPCR primer |
Commercial assay or kit | Nfatc1 | Thermo-Fisher Scientific | Mm00479445_m1 | qPCR primer |
Commercial assay or kit | Mfsd7c | Thermo-Fisher Scientific | Mm01302920_m1 | qPCR primer |
Commercial assay or kit | Mrps2 | Thermo-Fisher Scientific | Mm00475529_m1 | qPCR primer |
Commercial assay or kit | Runx2 | Thermo-Fisher Scientific | Mm00501584_m1 | qPCR primer |
Commercial assay or kit | Slc11a2 | Thermo-Fisher Scientific | Mm00435363_m1 | qPCR primer |
Commercial assay or kit | Slc39a14 | Thermo-Fisher Scientific | Mm01317439_m1 | qPCR primer |
Commercial assay or kit | Slc40a1 | Thermo-Fisher Scientific | Mm01254822_m1 | qPCR primer |
Commercial assay or kit | Slc46a1 | Thermo-Fisher Scientific | Mm00546630_m1 | qPCR primer |
Commercial assay or kit | Tfrc | Thermo-Fisher Scientific | Mm00441941_m1 | qPCR primer |
Commercial assay or kit | Tfr2 | Thermo-Fisher Scientific | Mm00443703_m1 | qPCR primer |
Bone marrow monocytes qProteomics.
Pre-osteoclasts qProteomics.
Osteoclasts qProteomics.
Ingenuity pathway analysis (IPA) of canonical pathways in osteoclasts.
Changes of cytoskeletal proteins in TfR1-null osteoclast lineage cells revealed by qProteomics.