Strain (Mus musculus) | Flt1LoxP/LoxP | The Jackson Laboratory | JAX: 028098 | Mouse line obtained from Guo-Hua Fong |
Strain (Mus musculus) | VEGFA+/Hyper | The Jackson Laboratory | JAX: 027314 | |
Strain (Mus musculus) | Pax7CreERT2/+ | The Jackson Laboratory | JAX: 017763 | |
Strain (Mus musculus) | mdx5cv | The Jackson Laboratory | JAX: 002379 | |
Strain (Mus musculus) | Flk1GFP | The Jackson Laboratory | JAX: 017006 | Mouse line obtained from Masatsugu Ema |
Strain (Mus musculus) | R26RtdT | The Jackson Laboratory | JAX: 007909 | |
Strain (Mus musculus) | VEGFALoxP/LoxP | | | Mouse line obtained from Napoleone Ferrara |
Genetic reagent | Lentiviral pCCL-E4ORF1 | PMID:20972423 | | Viral vector production |
Genetic reagent | Lentiviral pCCL-myrAkt1 | PMID:20972423 | | Viral vector production |
Genetic reagent | pCMV-VSV-G | Addgene | 8454 | Viral vector production |
Genetic reagent | pRSV-Rev | Addgene | 12253 | Viral vector production |
Genetic reagent | pMDLg/pRRE | Addgene | 12251 | Viral vector production |
Genetic reagent | RNAscope Probe - Mm-Flt1 (C1) | ACDBio | 415541 | RNAscope |
Cell line (Homo sapiens) | 293 FT | ThermoFisher Scoentific | R70007 | Viral vector production |
Cell line (Mus musculus) | C2C12 | American Type Culture Collection (ATCC) | CRL-1772 | MuSC line |
Cell line (Mus musculus) | bEnd.3 | American Type Culture Collection (ATCC) | CRL-2299 | EC line |
Cell line (Mus musculus) | C166 | American Type Culture Collection (ATCC) | CRL-2581 | EC line |
Antibody | Anti-CD31-PE (Rat monoclonal) | ThermoFisher Scientific | 12-0311-82; RRID: AB_465632 | 1:200 |
Antibody | Anti-CD45-PE (Rat monoclonal) | ThermoFisher Scientific | 12-0451-81; RRID: AB_465668 | 1:200 |
Antibody | Anti-Sca-1-PE (Rat monoclonal) | ThermoFisher Scientific | A18486; RRID: AB_2535332 | 1:200 |
Antibody | Anti-FLT1 (Mouse monoclonal) | R&D systems | MAB4711; RRID: AB_2107038 | 1:200 |
Antibody | Anti-FLT1-APC (Rat monoclonal) | R&D systems | FAB4711A; RRID: AB_622149 | 1:200 |
Antibody | Anti-FLK1 (Rat monoclonal) | BD Biosceinces | 555307; RRID: AB_395720 | 1:200 |
Antibody | Anti-FLK1-APC (Rat monoclonal) | BD Biosceinces | 560070; RRID: C28AB_1645226 | 1:200 |
Antibody | Anti-VE-cadherin (Rat monoclonal) | BD Biosciences | 555289; RRID: AB_2244723 | 1:200 |
Antibody | Anti-NRP1 (Rabbit monoclonal) | Cell Signaling Technology | 3725; RRID:AB_2155231 | 1:200 |
Antibody | Anti-NRP1-APC (Rat monoclonal) | R&D systems | FAB5994A | 1:200 |
Antibody | Anti-NRP2 (Rabbit monoclonal) | Cell Signaling Technology | 3366; RRID: AB_2155250 | 1:200 |
Antibody | Anti-NRP2-APC (Mouse monoclonal) | R&D systems | FAB22151A; RRID: AB_10973479 | 1:200 |
Antibody | Anti-Sca-1-PE (Rat monoclonal) | eBiosciences | 12-5981-81; RRID: AB_466085 | 1:200 |
Antibody | Anti-integrin a7-biotin (Mouse monoclonal) | Miltenyi Biotec | 130-101-979; RRID: AB_2652472 | 1:200 |
Antibody | Anti-IgG-APC (Mouse) | ThermoFisher Scientific | PA5-33237; RRID: AB_2550652 | 1:200 |
Antibody | Anti-IgG-APC (Rat) | ThermoFisher Scientific | 17-4321-81; RRID: AB_470181 | 1:200 |
Antibody | Anti-MyHC (Mouse monoclonal) | Developmental Study Hybridoma Bank | MF-20; RRID: AB_2147781 | 1:50 |
Antibody | Anti-MyoD (Mouse monoclonal) | DAKO | M3512; RRID: AB_2148874 | 1:200 |
Antibody | Anti-Pax7 (Mouse monoclonal) | Developmental Study Hybridoma Bank | PAX7; RRID: AB_528428 | 1:20 |
Antibody | Anti-pAKT (Rabbit monoclonal) | Cell Signaling | 4060; RRID: AB_2315049 | 1:500 |
Antibody | Anti-VEGFA (Rabbit monoclonal) | Abcam | Ab52917; RRID: AB_883427 | 1:500 |
Antibody | Anti-mouse IgG Alexa488 | ThermoFisher Scientific | A-11001; RRID: AB_2534069 | 1:1000 |
Antibody | Anti-rat IgG Alexa488 | ThermoFisher Scientific | A-11006; RRID: AB_141373 | 1:1000 |
Antibody | Anti-rabbit IgG Alexa488 | ThermoFisher Scientific | A-11008; RRID: AB_143165 | 1:1000 |
Antibody | Anti-rabbit IgG Alexa568 | ThermoFisher Scientific | A-11011; RRID: AB_143157 | 1:1000 |
Antibody | Anti-mouse IgG Alexa568 | ThermoFisher Scientific | A-11004; RRID: AB_2534072 | 1:1000 |
Antibody | Anti-rat IgG Alexa568 | ThermoFisher Scientific | A-11077; RRID: AB_2534121 | 1:1000 |
Antibody | Anti-biotin beads | Miltenyi Biotec | 130-090-485; RRID: AB_244365 | MACS (1:500) |
Antibody | Anti-PE beads | Miltenyi Biotec | 130-048-801; RRID: AB_244373 | MACS (1:500) |
Antibody | Anti-Activated Caspase-3 | Abcam | ab214430; RRID: AB_2938798 | 1:200 |
Antibody | Anti-phosphoAKT1 | Cell Signaling | 4060; RRID: AB_2315049 | 1:200 for IHC, 1:1000 for WB |
Antibody | Anti-GAPDH HRP conjugated | Cell Signaling | 3683; RRID: AB_1642205 | 1:10000 |
Antibody | Anti-eMHC (Mouse monoclonal) | Developmental Study Hybridoma Bank | F1.652; RRID: AB_528358 | 1:200 |
Antibody | Anti-Laminin-2 (Rat monoclonal) | MilliporeSigma | L0663; RRID: AB_477153 | 1:500 |
Sequence-based reagent (Oligo DNA) | TTAAACGAACGTACTTGCAGATG | Integrated DNA Technologies (IDT) | N/A | qPCR Vegfa 92 bp (Forward) |
Sequence-based reagent (Oligo DNA) | AGAGGTCTGGTTCCCGAAA | Integrated DNA Technologies (IDT) | N/A | qPCR Vegfa 92 bp (Reverse) |
Sequence-based reagent (Oligo DNA) | GCAGAGCCAGGAACATATACACA | Integrated DNA Technologies (IDT) | N/A | qPCR mFlt1 103 bp(Forward) |
Sequence-based reagent (Oligo DNA) | GAGATCCGAGAGAAAATGGCCTTT | Integrated DNA Technologies (IDT) | N/A | qPCR mFlt1 103 bp (Reverse) |
Sequence-based reagent (Oligo DNA) | GCAGAGCCAGGAACATATACACA | Integrated DNA Technologies (IDT) | N/A | qPCR sFlt1 73 bp (Forward) |
Sequence-based reagent (Oligo DNA) | CAGTGCTCACCTCTAACG | Integrated DNA Technologies (IDT) | N/A | qPCR sFlt1 73 bp (Reverse) |
Sequence-based reagent (Oligo DNA) | CAGTGGTACTGGCAGCTAGAAG | Integrated DNA Technologies (IDT) | N/A | qPCR KDR/Flk1 66 bp (Forward) |
Sequence-based reagent (Oligo DNA) | ACAAGCATACGGGCTTGTTT | Integrated DNA Technologies (IDT) | N/A | qPCR KDR/Flk1 66 bp (Reverse) |
Sequence-based reagent (Oligo DNA) | TCCTGGGAAACTGGTATATCTATGA | Integrated DNA Technologies (IDT) | N/A | qPCR Nrp1 75 bp (Forward) |
Sequence-based reagent (Oligo DNA) | CATTCCAGAGCAAGGATAATCTG | Integrated DNA Technologies (IDT) | N/A | qPCR Nrp1 75 bp (Reverse) |
Sequence-based reagent (Oligo DNA) | ATGGCTGGACACCCAATTT | Integrated DNA Technologies (IDT) | N/A | qPCR Nrp2 67 bp (Forward) |
Sequence-based reagent (Oligo DNA) | ATGGTTAGGAAGCGCAGGT | Integrated DNA Technologies (IDT) | N/A | qPCR Nrp2 67 bp (Reverse) |
Sequence-based reagent (Oligo DNA) | CACCTGGAGAGGATGAAGAAGAA | Integrated DNA Technologies (IDT) | N/A | qPCR Myh3 298 bp (Forward) |
Sequence-based reagent (Oligo DNA) | AAGACTTGACTTTCACTTGGAGTTTA TC | Integrated DNA Technologies (IDT) | N/A | qPCR Myh3 298 bp (Reverse) |
Sequence-based reagent (Oligo DNA) | TTCGGAAGCTCCTTCTGTTT | Integrated DNA Technologies (IDT) | N/A | qPCR Htatsf1 79 bp (Forward) |
Sequence-based reagent (Oligo DNA) | CCAGAGTCTGAATACAATGGTCA | Integrated DNA Technologies (IDT) | N/A | qPCR Htatsf1 79 bp (Reverse) |
Sequence-based reagent (Oligo DNA) | CGCACGGCCGGTACAGTGAAACTG | Integrated DNA Technologies (IDT) | N/A | qPCR 18 S rRNA 343 bp (Forward) |
Sequence-based reagent (Oligo DNA) | CGCACGGCCGGTACAGTGAAACTG | Integrated DNA Technologies (IDT) | N/A | qPCR 18 S rRNA 343 bp (Reverse) |
Sequence-based reagent (Oligo DNA) | CTTGCTCACCATGGTCAGCTGCTG | Integrated DNA Technologies (IDT) | N/A | Genotyping Myh3 Exon1 WT/MUT-103bp (Reverse) |
Sequence-based reagent (Oligo DNA) | CACTTTTAACTTCGACCCTGAGCC | Integrated DNA Technologies (IDT) | N/A | Genotyping Myh3 Exon2 WT/MUT-103bp (Reverse) |
Sequence-based reagent (Oligo DNA) | GGCCAGACTCTCTTTCTCAAGTGC | Integrated DNA Technologies (IDT) | N/A | Genotyping Myh3 Exon2 WT-135bp (Forward) |
Sequence-based reagent (Oligo DNA) | GCAGAATTGCCTGTTATCCCTCCC | Integrated DNA Technologies (IDT) | N/A | Genotyping Myh3 Exon3 WT-135bp (Reverse) |
Sequence-based reagent (Oligo DNA) | GCTGCTGTTGATTACCTGGC | Integrated DNA Technologies (IDT) | N/A | Genotyping Pax7CreERT2 (Common) |
Sequence-based reagent (Oligo DNA) | CAAAAGACGGCAATATGGTG | Integrated DNA Technologies (IDT) | N/A | Genotying Pax7CreERT2 MUT-235bp (Reverse) |
Sequence-based reagent (Oligo DNA) | CTGCACTGAGACAGGACCG | Integrated DNA Technologies (IDT) | N/A | Genotying Pax7CreERT2 WT-419bp (Reverse) |
Sequence-based reagent (Oligo DNA) | GGCATTAAAGCAGCGTATCC | Integrated DNA Technologies (IDT) | N/A | Genotying R26RtdT (9103) MUT-196bp (Forward) |
Sequence-based reagent (Oligo DNA) | CTGTTCCTGTACGGCATGG | Integrated DNA Technologies (IDT) | N/A | Genotying R26RtdT (9105) MUT-196bp (Reverse) |
Sequence-based reagent (Oligo DNA) | AAGGGAGCTGCAGTGGAGTA | Integrated DNA Technologies (IDT) | N/A | Genotying R26RtdT (9020) WT-297bp (Forward) |
Sequence-based reagent (Oligo DNA) | CCGAAAATCTGTGGGAAGTC | Integrated DNA Technologies (IDT) | N/A | Genotying R26RtdT (9021) WT-297bp (Reserve) |
Sequence-based reagent (Oligo DNA) | AGCAGCACGACTTCTTCAAGTCCG | Integrated DNA Technologies (IDT) | N/A | Genotying Flk1GFP-161bp (Forward) |
Sequence-based reagent (Oligo DNA) | CTCCTTGAAGTCGATGCCCTTCAG | Integrated DNA Technologies (IDT) | N/A | Genotying Flk1GFP-161bp (Reserve) |
Sequence-based reagent (Oligo DNA) | CGCTTTTTGTCAGTCATCTTCA | Integrated DNA Technologies (IDT) | N/A | Genotying Flt1Loxp/Loxp (FlpeEX3F) WT-223bp/MUT-641bp (Forward) |
Sequence-based reagent (Oligo DNA) | GTGCCACTGACCTAACATGTAAGAG | Integrated DNA Technologies (IDT) | N/A | Genotying Flt1Loxp/Loxp (FlpeInt3R) WT-223bp/MUT-641bp (Reverse) |
Sequence-based reagent (Oligo DNA) | CCA TAG ATG TGA CAA GCC AAG | Integrated DNA Technologies (IDT) | N/A | Genotyping VEGFAHyper (24286) WT-254bp (Forward) |
Sequence-based reagent (Oligo DNA) | ACC CGG GGA TCC TCT AGA AC | Integrated DNA Technologies (IDT) | N/A | Genotyping VEGFAHyper (25307) MUT-199bp (Forward) |
Sequence-based reagent (Oligo DNA) | GAC CGT GCT TGG TCA CCT | Integrated DNA Technologies (IDT) | N/A | Genotyping VEGFAHyper (25308, Common) WT-254bp/MUT-199bp (Reverse) |
Sequence-based reagent (Oligo DNA) | CCTGGCCCTCAAGTACACCTT | Integrated DNA Technologies (IDT) | N/A | VEGFALoxP/LoxP (muVEGF 419 .F) WT-106bp/MUT-148bp (Forward) |
Sequence-based reagent (Oligo DNA) | TCCGTACGACGCATTTCTAG | Integrated DNA Technologies (IDT) | N/A | VEGFALoxP/LoxP (muVEGF 567 .R) WT-106bp/MUT-148bp (Reverse) |
Sequence-based reagent (Oligo DNA) | GAAGCTCCCAGAGACAAGTC | Integrated DNA Technologies (IDT) | N/A | mdx5cv (0981) WT/MUT-180bp (Forward) |
Sequence-based reagent (Oligo DNA) | TCATGAGCATGAAACTGTTCTT | Integrated DNA Technologies (IDT) | N/A | mdx5cv (0981) WT/MUT-180bp (Reverse) |
Commercial assay or kit | GoTaq qPCR Master Mix | Promega, | A6002 | qPCR |
Commercial assay or kit | Click-iT EdU Alexa Fluor 488 Imaging Kit | Thermo Scientific | C10337 | EdU staining |
Commercial assay or kit | Crystal violet Assay Kit | Abcam | ab232855 | Cell viability assay |
Commercial assay or kit | Transcriptor First Strand cDNA Synthesis Kit | Roche-Sigma-Aldrich | 4379012001 | cDNA synthesis |
Commercial assay or kit | Annexin V-Biotin Apoptosis Detection Kit | eBioscience | BMS500BT-100 | Appotosis assay |
Commercial assay or kit | DirectZolTM RNA Microprep Kit | Zymo Research | R2062 | RNA isolation |
Commercial assay or kit | Midi Fast Ion Plasmid Kit | IBI Scientific | IB47111 | Plasmid DNA isolation |
Commercial assay or kit | H&E Staining Kit | Abcam | ab245880 | Histology |
Commercial assay or kit | SuperSignal West Femto chemiluminescent substrate | Fisher Scientific | PI37074 | Western Blotting |
Commercial assay or kit | NE-PER Nuclear and Cytoplasmic Extraction reagents | Thermo Fisher Scientific | 78833 | Protein extraction |
Chemical compound, drug | bFGF | Thermo Fisher Scientific | PHG0263 | MuSC culture (20 ng/ml) |
Chemical compound, drug | Bovine serum albumin (BSA) | Jackson Immuno Research | 10001620 | FACS/Immunostaining (1–2%) |
Chemical compound, drug | Chicken embryo extract | MP-Biomedical | 92850145 | Single muscle fiber culture (0.5%) |
Chemical compound, drug | Collagen | BD Biosciences | 354236 | Culture dish coating (0.01%) |
Chemical compound, drug | Collagenase type I | Sigma-Aldrich | C0130 | Single myofiber isolation (0.2%) |
Chemical compound, drug | Collagenase type II | Worthington Biochemical Corp | CLD-2 | FACS and MuSC/ES isolation (0.2%) |
Chemical compound, drug | DAPI (4’,6-diamidino-2-phenylindole dihydrochloride) | Thermo Fisher Scientific | D1306 | DNA staining (1 µg/ml) |
Chemical compound, drug | Dulbecco’s Modified Eagle’s Medium (DMEM) | Thermo Fisher Scientific | 41966 | Single myofiber and ES culture |
Chemical compound, drug | EdU | Thermo Fisher Scientific | C10340 | Cell proliferation (1 μM) |
Chemical compound, drug | F-10 Ham's media | Sigma-Aldrich | N6635 | MuSC culture |
Chemical compound, drug | Fetal calf serum (FCS) | Atlas Biological | FS-0500-AD | MuSC/EC/Single muscle fiber culture (10% or 20%) |
Chemical compound, drug | Horse serum | Thermo Fisher Scientific | 26050088 | 2% (coating), 5% (Differentiation culture) |
Chemical compound, drug | L-glutamine | Thermo Fisher Scientific | 25030 | Culture medium (20 mM) |
Chemical compound, drug | Matrigel | Corning Life Sciences | 354230 | Single muscle fiber culture (1:20) |
Chemical compound, drug | Penicillin/streptomycin | Life Technologies | 15140 | Culture medium (1 X) |
Chemical compound, drug | Tamoxifen (TMX) | Sigma-Aldrich | T5648 | Cre recombinase (60 mg/kg i.p.) |
Chemical compound, drug | 4-hydroxy tamoxifen (4-OHT) | Sigma-Aldrich | H6278 | Culture (1 µM) |
Chemical compound, drug | Cardiotoxin (CTX) | Sigma-Aldrich | V9125 | Muscle injury (10 µM) |
Chemical compound, drug | DraIII | New England Biolabs | R3510S | DNA digestion (500 U/ml) |
Chemical compound, drug | ZombieNIR | Biolegends | 423105 | FACS (0.1%) |
Chemical compound, drug | recombinant VEGFA | R&D Systems | 493 MV | Culture (2–100 ng/ml) |
Chemical compound, drug | Thapsigargin | Sigma-Aldrich | T9033 | Culture (1 µM) |
Chemical compound, drug | FLT1-FC | R&D Systems | 7756-FL | Culture (100 ng/ml) |
Chemical compound, drug | ZM306416 | R&D Systems | 2499/1 | Culture (3 µM) |
Chemical compound, drug | SU5402 | R&D Systems | 3300/1 | Culture (10 µM) |
Chemical compound, drug | EG00229 | R&D Systems | 6986/10 | Culture (30 µM) |
Chemical compound, drug | PolyJet transfection reagent | Signagen Laboratories | SL100688 | DNA transfection (10 µl for 6 cm plate) |
Chemical compound, drug | Polybrene | MilliporeSigma | H9268 | Viral infection (0.8 μg/ml) |
Chemical compound, drug | Saponin | ThermoFisher Scientific | ICN10285525 | Immunostaining (0.01%) |
Chemical compound, drug | Direct Red 80 | Sigma-Aldrich | 365548 | Histology (0.1%) |
Chemical compound, drug | Proteinase K | Sigma-Aldrich | P2308 | DNA isolation (40 µg/mL) |
Chemical compound, drug | Oil Red O solution | Sigma-Aldrich | O1391-250ML | Histology (0.5%) |
Chemical compound, drug | Trizol | ThermoFisher Scientific | 15596026 | RNA isolation |
Software, algorithm | Photoshop 2020 | Adobe | https://www.adobe.com/products/photoshop.html | Imaging analysis |
Software, algorithm | Fiji | NIH | https://imagej.net/software/fiji/ | Imaging analysis |
Software, algorithm | cellSens Entry 1.11 | Olympus | https://www.olympus-lifescience.com/en/software/cellsens/ | Microscopy |
Software, algorithm | Prism 9 | GraphPad | https://www.graphpad.com/support/faq/prism-900-release-notes/ | Data analysis and statistics |
Software, algorithm | RStudio | RStudio | https://www.rstudio.com | Data analysis and statistics |
Software, algorithm | Autodesk Graphic | Autodesk | https://www.graphic.com | Vector design |
Software, algorithm | BioRender: Scientific Image and Illustration Software | BioRender.com | https://www.biorender.com | Illustrator |
Other | Anti-biotin beads | Miltenyi Biotec | 130-090-485; RRID:AB_244365 | MACS |
Other | Anti-PE beads | Miltenyi Biotec | 130-048-801; RRID:AB_244373 | MACS |
Other | Cell culture plate, 24 well | Sarstedt | 83.3922 | Single myofiber culture |
Other | Tissue culture dish | Sarstedt | 83.39 | MuSC/ES culture |
Other | Tissue culture dish | Sarstedt | 83.3901 | MuSC/ES culture |
Other | Tissue culture dish | Sarstedt | 83.3902 | MuSC/ES culture |
Other | LD column | Miltenyi Biotec | 130-042-901 | MACS |
Other | MS column | Miltenyi Biotec | 130-042-201 | MACS |
Other | Fortessa X-20 | BD Biosciences | | FACS |
Other | BD FACSAria | BD Biosciences | | FACS |
Other | moorLabTM laser Doppler | Moor Instruments | MOORVMS-LDF | Laser Doppler flow |
Other | Grip strength meter | Columbus Instruments | 1027CSM-D54 | Forelimb muscle force |
Other | iBright FL1500 | ThermoFisher Scientific | A44115 | Western blotting |
Other | Olympus IX81 Inverted Fluorescense microscope | Olympus | | Microscope |
Other | Olympus BX51 Fluorescense microscope | Olympus | | Microscope |