Brucella activates the host RIDD pathway to subvert BLOS1-directed immune defense
Abstract
The phagocytosis and destruction of pathogens in lysosomes constitute central elements of innate immune defense. Here, we show that Brucella, the causative agent of brucellosis, the most prevalent bacterial zoonosis globally, subverts this immune defense pathway by activating regulated IRE1α-dependent decay (RIDD) of Bloc1s1 mRNA encoding BLOS1, a protein that promotes endosome–lysosome fusion. RIDD-deficient cells and mice harboring a RIDD-incompetent variant of IRE1α were resistant to infection. Inactivation of the Bloc1s1 gene impaired the ability to assemble BLOC-1-related complex (BORC), resulting in differential recruitment of BORC-related lysosome trafficking components, perinuclear trafficking of Brucella-containing vacuoles (BCVs), and enhanced susceptibility to infection. The RIDD-resistant Bloc1s1 variant maintains the integrity of BORC and a higher-level association of BORC-related components that promote centrifugal lysosome trafficking, resulting in enhanced BCV peripheral trafficking and lysosomal destruction, and resistance to infection. These findings demonstrate that host RIDD activity on BLOS1 regulates Brucella intracellular parasitism by disrupting BORC-directed lysosomal trafficking. Notably, coronavirus murine hepatitis virus also subverted the RIDD–BLOS1 axis to promote intracellular replication. Our work establishes BLOS1 as a novel immune defense factor whose activity is hijacked by diverse pathogens.
Editor's evaluation
To successfully replicate in the host cell, Brucella must evade degradation in lysosomes and traffic to the ER. This work uncovers a novel mechanism by which Brucella harnesses the host unfolded protein response to degrade Blos 1, a key regulator of lysosomal trafficking, thereby enabling pathogenic Brucella peri-nuclear/ER trafficking.
https://doi.org/10.7554/eLife.73625.sa0Introduction
Brucella is an intracellular vacuolar pathogen that invades many cell and tissue types, including nonprofessional and professional phagocytes (de Figueiredo et al., 2015). Brucellosis has eluded systematic attempts at eradication for more than a century (Godfroid et al., 2002), and even in most developed countries, no approved human vaccine is available (Ficht and Adams, 2009). The intracellular lifestyle limits exposure to host innate and adaptive immune responses and sequesters the organism from the effects of some antibiotics. Brucella evades intracellular destruction by limiting interactions of the Brucella-containing vacuole (BCV) with the lysosomal compartment (Criscitiello et al., 2013; Pizarro-Cerdá et al., 1998). BCVs harboring internalized Brucella traffic from endocytic compartments (eBCVs) to a replicative niche within vacuoles (rBCVs) that are decorated with markers of the endoplasmic reticulum (ER) (Pizarro-Cerdá et al., 1998; Starr et al., 2012). BCVs also accumulate autophagic membranes (aBCVs), which constitute a distinctive aspect of the intracellular lifestyle of the pathogen (Pandey et al., 2018; Starr et al., 2012). The VirB type IV secretion system (T4SS) is a significant virulence factor that regulates Brucella intracellular trafficking (Marchesini et al., 2011; Paredes-Cervantes et al., 2011; Sá et al., 2012; Smith et al., 2012). Brucella effectors secreted by the T4SS promote bacterial intracellular trafficking and growth via modulation of host functions (de Barsy et al., 2011; de Jong et al., 2008; Döhmer et al., 2014; Miller et al., 2017; Myeni et al., 2013) and organisms that lack this system fail to establish productive infections.
The Unfolded Protein Response (UPR) is an evolutionarily conserved signaling pathway that allows the ER to recover from the accumulation of misfolded proteins (Gardner et al., 2013; Walter and Ron, 2011) during ER stress. The UPR signals through the stress sensors IRE1α, ATF6, and PERK located in the ER membrane. When the luminal domains of these proteins sense unfolded proteins, they transduce signals to their cytoplasmic domains, which initiate signaling that ultimately results in UPR (Lee et al., 2008). IRE1α plays a central role in triggering UPR through an endonuclease/RNase activity in its cytoplasmic tail that catalyzes the splicing of Xbp1 mRNA, which is then translated to generate the XBP1 transcription factor (Lee et al., 2008; Ron and Walter, 2007). IRE1α RNase activity can also cleave a wide variety of cellular mRNAs that leads to their degradation in a process termed regulated IRE1-dependent mRNA decay (RIDD) (Hollien and Weissman, 2006). The RIDD pathway displays selectivity. For example, the pathway cleaves a specific subset of mRNAs encoding polypeptides destined for cotranslational translocation into the ER lumen. The degradation of these mRNAs supports ER homeostasis by reducing the flux of nonessential polypeptides into the ER (Hollien and Weissman, 2006). The molecular targets of RIDD activity, and the physiological roles that this process plays in cells, remain areas of investigation.
Brucella infection induces host cell ER stress and activates host UPR (de Jong et al., 2013; Pandey et al., 2018; Smith et al., 2013; Taguchi et al., 2015; Wang et al., 2016). The UPR sensor IRE1α, but neither PERK nor ATF6, is required for the intracellular replication of the pathogen (Qin et al., 2008; Taguchi et al., 2015), indicating that the IRE1α signaling pathway confers susceptibility to host cell parasitism. An IRE1α–ULK1 signaling axis also contributes to conferring susceptibility to Brucella intracellular replication; IRE1α-directed activation of components of the host autophagy program promotes proper bacterial intracellular trafficking and replication (Pandey et al., 2018). Despite the abovementioned advances, our understanding of how the IRE1α–RIDD axis and downstream processes regulate the intracellular lifestyle of Brucella remains largely unknown.
BLOS1 [biogenesis of lysosome-related organelles complex-1 (BLOC-1) subunit 1, also known as BLOC1S1/GCN5L1], a subunit of both the BLOC-1 and the BLOC-1-related complex (BORC), plays diverse roles in cells, including mitochondrial protein acetylation, modulation of metabolic pathways, and endosome–lysosome trafficking and fusion (Bae et al., 2019; Guardia et al., 2016; Pu et al., 2017; Pu et al., 2015). In mammalian cells, BLOS1 has also been shown to be a principal target of RIDD activity (Bae et al., 2019; Bright et al., 2015; Hollien et al., 2009) and is required for host cell cytotoxicity induced by Ebola virus (Carette et al., 2011). To date, the roles and mechanisms by which BLOS1 controls infection by intracellular pathogens remain largely unknown. Here, we demonstrate that IRE1α-directed Bloc1s1 mRNA degradation confers susceptibility to Brucella infection. Brucella-induced RIDD activity suppresses Bloc1s1 expression, disassembles BORC components, and limits BLOS1-regulated interactions between BCVs and lysosomes. In addition, we show that murine hepatitis virus (MHV), a betacoronavirus, also subverts BLOS1 activity to promote its intracellular replication. Collectively, these activities promote the productive subcellular trafficking and intracellular replication of diverse pathogens. Our findings, therefore, identify BLOS1 as a novel immune defense factor that defends against bacterial and viral infection and show that Brucella and MHV subvert this innate immune defense system to promote disease.
Results
Ern1 conditional knockout mice are resistant to Brucella infection
Brucella induces host cell UPR during infection (Qin et al., 2008; Taguchi et al., 2015) and activates an IRE1α-to-autophagy signaling axis in host cells to promote its intracellular lifestyle (Pandey et al., 2018). To extend these findings to an in vivo model of brucellosis, we tested the hypothesis that UPR and IRE1α confer susceptibility to Brucella infection in mice harboring a conditional mutation in Ern1, the gene encoding IRE1α. Because mice homozygous for null mutations in Ern1 display embryonic lethality during organogenesis, we used a control Lyz2-Ern1wt/wt [henceforth, wt (wild-type)-IRE1α] and a Ern1 conditional knockout (CKO mouse line (Ern1flox/flox; Lyz2Cre/+, hence after, m-IRE1α)) (Figure 1—figure supplement 1A) (gift from the Iwawaki lab) in the experiments. In this line, exons 20–21 of the gene encoding IRE1α were deleted in monocytes and macrophages, generating animals in which the endonuclease domain (and hence RIDD activity) was specifically disrupted. However, the kinase domain remained intact (Hur et al., 2012; Iwawaki et al., 2009). Macrophages are critical cellular targets for Brucella colonization (de Figueiredo et al., 2015). Hence, the tissue and molecular specificity of this lesion rendered the m-IRE1α mouse an ideal system for investigating how bacterial activation of host RIDD activity controls intracellular parasitism by the virulent B. melitensis strain 16M (Bm16M).
After confirming the RNase activity deficiency of the truncated IRE1α in bone marrow-derived macrophages (BMDMs) from m-IRE1α animals (Figure 1—figure supplement 1B), we verified that m-IRE1α mice had normal organ morphologies, fertility, growth, and development. In addition, we showed that these animals had similar B cell (B220+), T cell (CD4+ or CD8+), and CD11b+ profiles (Figure 1—figure supplement 1C). We also showed that the IL-1β, IL-6, and TNF-α responses of BMDMs to LPS stimulation were reduced in mutant mice (Figure 1A–C), consistent with previous findings that these LPS-mediated responses are controlled, in part, by IRE1α activity (Martinon et al., 2010).
To determine whether IRE1α activity in macrophages contributed to pathogen burden, dissemination, and disease progression, we infected wt-IRE1α control and m-IRE1α mice with Bm16M via the intraperitoneal route, humanely sacrificed the mice at various times postinfection, and then determined the bacterial burden in assorted tissues by quantifying the number of recovered colony-forming units (CFUs). We found that tissue-specific mutation of IRE1α resulted in enhanced resistance to bacterial infection with significant reductions in bacterial load in the spleen and liver compared to wt-IRE1α controls at 7 or 14 days postinfection (dpi), respectively (Figure 1D, E). However, both infected wt- and m-IRE1α mice displayed similar spleen weights (Figure 1—figure supplement 1D, E) and spleen or liver inflammation (Figure 1F–H), revealing that the lower numbers of CFU recovered from m-IRE1α animals were not accompanied by corresponding decreases in inflammation. To test the hypothesis that the differential bacterial burden in macrophage cells, the predominant cell type in which the pathogen resides and replicates in vivo, accounted for this reduction, we compared the bacterial load in CD11b+ cells from control and m-IRE1α mice that had been infected with Bm16M for 7 days. We found that indeed CD11b+ cells from the spleens of m-IRE1α mice displayed striking reductions in bacterial load (Figure 1I), thereby suggesting that the resistance of these cells to intracellular parasitism contributed to the resistance phenotype observed at the organismal level. Divergent Brucella species display distinct host preferences; however, their interactions with host cells share common features (de Figueiredo et al., 2015). To test the hypothesis that m-IRE1α mice also displayed resistance to infection by other Brucella species, we infected these mice with B. abortus strain S2308 (BaS2308), a strain that displays tropism for cattle. We then assessed tissue burden in spleen and liver at 7 dpi. We found that m-IRE1α mice also exhibited resistance to BaS2308 infection (Figure 1J), thereby indicating that the resistance phenotype of the mutant mice was not pathogen species specific.
IRE1α RNase activity confers susceptibility to Brucella infection
Xbp1 splicing was dramatically diminished in BMDMs from m-IRE1α mice (Figure 1—figure supplement 1B), indicating that BMDMs from the m-IRE1α mice carried the expected functional defects in IRE1α RNase activity. We thus tested the hypothesis that IRE1α RNase activity confers susceptibility to intracellular parasitism by Bm16M. First, we performed CFU assays of Bm16M infection of BMDMs from m-IRE1α and control mice and found that the replication efficiency of Bm16M in m-IRE1α BMDMs at 8, 24, and 48 h.p.i. was significantly lower than controls (Figure 1K). Similar results were observed in control and Ern1−/− mouse embryonic fibroblasts (MEFs) (Qin et al., 2008; Figure 1—figure supplement 2A-B). Second, we observed fewer Bm16M were recovered from BMDMs or RAW264.7 macrophages treated with 4μ8C, a compound that specifically antagonizes IRE1α RNase activity while leaving its kinase activity intact (Cross et al., 2012), than mock-treated controls (Figure 1L–M). These findings supported the hypothesis that IRE1α RNase activity confers susceptibility to infection by regulating the intracellular trafficking and/or survival of the pathogen.
IRE1α regulation of Bm16M intracellular replication is XBP1 independent
We performed additional experiments to interrogate the role of IRE1α RNase activity in controlling Brucella infection. Two possibilities were explored: (1) IRE1α catalyzed splicing of Xbp1 transcripts, and downstream expression of XBP1-responsive genes, conferred susceptibility to Bm16M infection; or (2) IRE1α catalyzed RIDD activity controlled this process. To determine whether Xbp1 splicing was the reason, we examined the survival and intracellular replication of the pathogen in BMDMs harvested from Xbp1−/− mice (gift from the Glimcher lab) in which Xbp1 was conditionally ablated from monocytes, macrophages, and granulocytes (henceforth, ∆Xbp1 mice) and found that Bm16M replicated similarly in ∆Xbp1 BMDMs and wt-Xbp1 littermate controls (Figure 1N). Moreover, Bm16M displayed similar levels of liver and spleen colonization in ∆Xbp1 and wt-Xbp1 mice (Figure 1O, P). These data suggested an XBP1-independent role for IRE1α RNase activity in conferring susceptibility to Bm16M infection, thereby implicating RIDD as the sought-after activity (see below).
IRE1α activity regulates Brucella intracellular trafficking and replication
To dissect the mechanism by which IRE1α activity confers susceptibility to intracellular parasitism by Bm16M, we examined this process in BMDMs derived from m-IRE1α mice. We observed that the expression level of IRE1α was relatively unchanged during a time course (48 hr) of infection in wt- or m-IRE1α BMDMs and MEFs (Figure 1Q, R; Figure 1—figure supplement 2C-D). As expected, CKO of IRE1α did not impair IRE1α phosphorylation in response to Brucella infection (Figure 1—figure supplement 2E). IRE1α phosphorylation was enhanced over the same time course in wt-IRE1α BMDMs during Bm16M infection (Figure 1S,T). Host IRE1α activity was also required for Brucella intracellular parasitism (Figure 1K; Figure 1—figure supplement 2). To test whether IRE1α regulates Bm16M intracellular trafficking, we used confocal immunofluorescence microscopy (CIM) to analyze the localization of the pathogen in Ern1+/+ and Ern1−/− MEFs, or m-IRE1α and control BMDMs. In IRE1α harboring controls, the pathogen transiently trafficked through early and late endosomes (EEA1+ and M6PR+ compartments, respectively) (Figure 2A–D; Figure 2—figure supplement 1A–D) before primarily accumulating (at 24 and 48 h.p.i.) in a replicative niche decorated with the ER marker calreticulin (Figure 2E, F; Figure 2—figure supplement 1E and G); however, in m-IRE1α BMDMs or Ern1−/− MEF cells, Bm16M displayed reduced trafficking to calreticulin+ compartments (Figure 2E, F; Figure 2—figure supplement 1E, G). Instead, the pathogen trafficked with greater efficiency to M6PR+ late endosomes (at 12 h.p.i.) (Figure 2B, D; Figure 2—figure supplement 1B, D), and to LAMP1+ or cathepsin D+ lysosomes (at 24 and 48 h.p.i.) (Figure 2G–J; Figure 2—figure supplement 1F, H). Our data, therefore, demonstrated that IREα activity controls Bm16M intracellular replication, likely via regulation of BCV ER trafficking. These findings encouraged us to investigate the molecular mechanisms driving these phenomena.
Brucella infection downregulates RIDD genes
We were intrigued with the hypothesis that Bm16M subverts host RIDD activity to promote intracellular parasitism. First, since host UPR/IRE1α RNase activity is induced by Brucella effectors secreted by the T4SS of the pathogen (de Jong et al., 2013), we tested whether RIDD activity was dependent upon the Brucella T4SS. We found that induction of IRE1α RNase activity occurred in a Brucella T4SS-dependent fashion (Figure 3—figure supplement 1A). Next, we performed RNA-seq analysis to define candidate host genes whose transcripts were subject to RIDD control during Brucella infection. Specifically, we used Bm16M to infect triplicate sets of BMDMs as follows: (1) solvent control-treated wt-IRE1α, (2) 4μ8C-treated wt-IRE1α, or (3) solvent control-treated m-IRE1α (Figure 3—figure supplement 1B). At 4 or 24 h.p.i., we harvested host mRNA for RNA-seq analysis. Differential expression analysis was then performed to identify genes that were downregulated following Bm16M infection of wt-IRE1α cells but were unchanged or upregulated in either infected, drug-treated cells, or infected, m-IRE1α cells. Genes that displayed reduced expression (p < 0.05) in response to infection at 4 and/or 24 h.p.i., and also whose infection-dependent reductions in expression were reversed upon treatment with 4μ8C, or in m-IRE1α cells, were defined as candidate ‘RIDD genes’. This analysis resolved 847 candidate RIDD genes (Figure 3A–C; Figure 3—figure supplement 1C–F; Figure 3—figure supplement 2; Figure 3—source data 1). KEGG pathway and interaction network analyses revealed that most RIDD candidate genes were involved in cellular component organization and biogenesis, RNA metabolism, and oxidative phosphorylation (Figure 3C; Figure 3—figure supplement 2).
We performed several experiments or analyses to validate candidate RIDD genes identified in the RNA-seq analysis. First, we compared our list of candidate RIDD genes to genes previously reported to be subject to RIDD control (Bright et al., 2015; Han et al., 2009; Hollien et al., 2009; So et al., 2012). This comparison identified 40 genes that were previously shown to be substrates of IRE1α RNase activity and/or displayed expression patterns consistent with RIDD targeting (Figure 3A, B). Second, we used real-time quantitative reverse transcription-PCR (qRT-PCR) to measure the expression levels of several candidate RIDD genes, including Bloc1s1, Cd300lf, Diras2, and Txnip (Figure 3D–G; Figure 3—figure supplement 3) during infection. We found that the expression of these genes was significantly lower in Brucella-infected wt-IRE1α cells than in m-IRE1α cells (Figure 3D–G). Third, we examined whether similar reductions in expression of candidate RIDD genes were observed in host cells infected with B. abortus S19 (BaS19, a vaccine strain) or BaS2308. We found that BaS19 or BaS2308 induced similar phenotypes as Bm16M (Figure 3—figure supplement 3A–D), suggesting that the phenotype was not species specific. To determine whether RIDD-mediated Txnip expression was also regulated by the microRNA miR17, a molecule that affects TXNIP mRNA stability (Lerner et al., 2012), we measured the expression of miR-17 via qRT-PCR. We found that the expression of miR-17 was reduced (Figure 3—figure supplement 3E). Fourth, we used qRT-PCR to test the hypothesis that heat-killed bacteria induced similar changes in RIDD gene expression. We found that heat-killed bacteria did not cause a similar effect. Hence, the induction of RIDD activity in host cells required interactions with the viable agent (Figure 3—figure supplement 3F) and was also T4SS dependent (Figure 3—figure supplement 1A). Finally, we found that the expression of the key RIDD gene Bloc1s1 was also reduced in ∆Xbp1 BMDMs infected with Bm16M, or when ER stress was induced in these cells (Figure 3H). These data suggested that the observed changes in host gene expression patterns were not a consequence of alterations in XBP1 transcription factor activity. Taken together, these data supported the hypotheses that (1) Bm16M infection induces RIDD activity in host cells and (2) RIDD activity confers enhanced susceptibility to intracellular parasitism by Brucella. However, these findings left open the question of the molecular mechanism by which RIDD activity controlled Bm16M replication.
RIDD activity on Bloc1s1 controls Brucella intracellular parasitism
Our RNA-seq analysis identified Bloc1s1 as a Brucella-induced RIDD gene. BLOS1 is a subunit of both the BLOC-1 and BORC complexes and plays diverse roles in cell physiological and biological processes, including endosome–lysosome trafficking and fusion (Figure 3C; Bae et al., 2019; Guardia et al., 2016; Pu et al., 2017; Pu et al., 2015). However, the mechanisms by which Bloc1s1 regulates microbial infection are largely unknown. This fact encouraged us to test the hypothesis that Bloc1s1 plays a central role in regulating Bm16M intracellular parasitism. First, we generated a cell line carrying a nonfunctional Bloc1s1 mutant allele (mBloc1s1). Mammalian BLOS1 contains three conserved XAT hexapeptide-repeat motifs that are essential for acetyltransferase activity and may also be a necessary structure-defining feature for acetyl-CoA contact (Scott et al., 2018; Wu et al., 2021a). Using CRISPR/cas9-mediated gene editing, we mutated the first XAT hexapeptide-repeat motif, which in the wild-type encodes ‘EALDVH’, and in the mutant encodes ‘EVVDH or EVDH’ (Figure 4—figure supplement 1A, Supplementary file 1). A cell line containing gene encoding Cas9 and a nonspecific gRNA was used as a control of the mBloc1s1 mutant line. Second, we generated a RIDD-resistant Bloc1s1 cell line (henceforth Rr-Bloc1s1). In this line, a mutation (from ‘G’ to ‘U’) was introduced into Bloc1s1 mRNA stem-loop structure (i.e., the target of IRE1α RNase activity) that rendered the mutated Bloc1s1 mRNA stem-loop structure-resistant cleavage by IRE1α RNase. A Bloc1s1::Bloc1s1-HA line that overexpresses Bloc1s1 (wt-Bloc1s1) served as a control of the Rr-Bloc1s1 cell line (Figure 4—figure supplement 1B; Supplementary file 1).
We characterized the developed cell lines in several ways. First, we noted that α-tubulin acetylation levels had been reported to be controlled, in part, by BLOS1 activity levels (Wu et al., 2018). Therefore, we monitored α-tubulin acetylation to assess whether our developed cell lines did, in fact, display alterations in BLOS1 activity. We found that mBloc1s1 and Rr-Bloc1s1 cells displayed reduced levels (Figure 4A) and maintained relatively higher levels (Figure 4B), respectively, of acetylated α-tubulin, compared to their corresponding controls. These data supported the hypothesis that these cells had the expected levels of BLOS1 activity. Second, we tested the replication of the pathogen in different Bloc1s1 cell lines. We found that mBloc1s1 cells exhibited increased susceptibility to Bm16M infection (Figure 4C), whereas Rr-Bloc1s1 cells or wild-type controls treated with 4μ8C supported dramatically reduced intracellular bacterial growth (Figures 1M and 4D; Figure 4—figure supplement 1C). Finally, we monitored the expression levels of BLOS1 protein during a 48-hr time course of infection. We found that BLOS1 expression was reduced at 16 h.p.i., and continuously decreased during Bm16M infection in wt-IRE1α control cells; however, in m-IRE1α BMDMs, BLOS1 expression was relatively stable or increased (at 48 h.p.i.) (Figure 4E). Similar results were observed in 4μ8C treated or untreated mBloc1s1, Rr-Bloc1s1, and control cells infected with BaS2308 (Figure 4—figure supplement 1D, E). These data demonstrate that low or high BLOS1 expression levels promote or impair Brucella infection, respectively.
BLOS1 regulates Brucella intracellular trafficking
The mechanism by which BLOS1 regulates Brucella infection was unknown. However, the observed subcellular trafficking defect of the pathogen in host cells harboring mutant or deficient variants of IREα (Figure 2; Figure 2—figure supplement 1) suggested that BLOS1 may control the intracellular parasitism of the pathogen by regulating its subcellular trafficking. To illuminate this aspect, we first characterized the mBloc1s1, Rr-Bloc1s1, and the corresponding control cell lines by treating them with tunicamycin (Tm, an UPR inducer) or 4μ8C, or infected them with Bm16M. We then assessed the trafficking of the pathogen in these cells using CIM. We found that low levels of BLOS1 or nonfunctional BLOS1 in uninfected or infected cells were associated with the accumulation of late endosome/lysosome (LE/Lys) membranes in the vicinity of nuclei, reduced colocalization of latex beads with cathepsin D, and increased perinuclear LC3b index or autophagic activity near nuclei, in both control and Tm-treated conditions (Figure 4—figure supplement 2A, C, E, G). In these studies, the LC3b index was defined as: (Total number of identified cells with the ratio of the mean LC3b intensity in the cytoplasm to that in the nucleus <1)/(Total number of the analyzed cells). In contrast, overexpression of BLOS1 (the wild-type cells expressing wt-Bloc1s1 or Rr-Bloc1s1) reduced LE/Lys perinuclear accumulation, increased the localization of latex beads in cathepsin D+ compartments, and reduced perinuclear autophagic activity (Figure 4—figure supplement 2B, D, F, H). These data indicated that cells deficient in BLOS1 or with RIDD resistance differentially control lysosome intracellular trafficking and autophagic activity. Although significant inhibition of BCV trafficking to lysosomes in the mBloc1s1 cells was not observed at 24 and 48 h.p.i., the mBloc1s1 cells supported enhanced BCV trafficking to ER compartments during bacterial infection, compared to that in the wild-type control cells, or 4μ8C-treated mBloc1s1 cells (Figure 4F–H). In contrast, Rr-Bloc1s1 cells displayed reduced BCV trafficking to ER compartments, but instead promoted BCVs trafficking to lysosomes during Bm16M infection, compared to controls (Figure 4I–K).
To test the hypothesis that Bm16M infection alters the dynamics of associations between BLOS1 and BCVs, we used CIM approaches to localize these elements during a time course of infection after confirmation of the specificities of antibodies used in the work (see below) (Figure 4—figure supplement 3A–D). We found higher levels of BLOS1 colocalization with BCVs in 4μ8C-treated control or mBloc1s1 cells than their corresponding untreated cells (Figure 4L); moreover, at 24 h.p.i., lower levels of BLOS1+ BCVs were observed in mBloc1s1 cells compared to wild-type controls (Figure 4—figure supplement 3E, F). Rr-Bloc1s1 and/or 4μ8C inhibition of Bloc1s1 degradation in host cells (i.e., Rr-Bloc1s1, 4μ8C-treated wt-Bloc1s1 control or Rr-Bloc1s1 cells) significantly promoted BLOS1+ BCVs compared to the wt-Bloc1s1 control (Figure 4L, Figure 4—figure supplement 3E, F). These findings demonstrated that nonfunctional BLOS1 permitted the trafficking of the pathogen from LE/Lys membranes to the ER. However, Rr-Bloc1s1 cells (or 4μ8C-treated cells) promoted the trafficking and degradation of the pathogen in lysosomes.
Disassembly of BORC promotes BCV trafficking to and accumulation in the ER
To test whether BORC-related lysosome trafficking components mediate BCV trafficking during infection, we analyzed the dynamics of the interaction of BCVs with LAMTOR1 (a central component of mTORC1), the small GTPase ARL8b, and kinesin KIF1b and KIF5b proteins during infection. During bacterial intracellular trafficking and replication, colocalization of BCVs with both LAMP1 and LAMTOR1, LAMP1, or LAMTOR1 decreased in control and mBloc1s1 cells, whereas these interactions were observed at higher levels in cells harboring Rr-Bloc1s1 variants (Figure 5A, B, E, G; Figure 4—figure supplement 3E; Figure 5—figure supplement 1A, B). Similarly, recruitment of ARL8b to BCVs and/or LAMP1 was reduced in control and mBloc1s1 cells, which impaired their kinesin-dependent movement toward the cell periphery; however, these interactions were maintained in Rr-Bloc1s1 cells (Figure 5C, D, F, H; Figure 5—figure supplement 1C-D). KIF1b+ and KIF5b+ preferentially drive lysosomes on peripheral tracks and perinuclear/ER tracks, respectively (Guardia et al., 2016). In control, mBloc1s1, and wt-Bloc1s1 cells, BCV interactions with KIF1b+ or KIF5b+ decreased (Figure 6A, B, E, G; Figure 6—figure supplement 1A, B) or increased (Figure 6C, D, F, H; Figure 6—figure supplement 1C-D), respectively. However, the opposite interaction phenomena were observed in Rr-Bloc1s1 cells (Figure 6; Figure 6—figure supplement 1). These findings suggest that BORC-related lysosome trafficking components may regulate BCV perinuclear trafficking, fusion with ER membranes and subsequent bacterial replication.
BORC, a protein complex that contains three components (i.e., BLOS1, BLOS2, SNAPIN) shared with the BLOC-1 complex and five other proteins KXD1, C17orf59 (Lyspersin), LOH12CR1(Myrlysin), C10orf32 (Diaskedin), and MEF2BNB, plays a critical role in the regulation of lysosome positioning (Pu et al., 2015). In HeLa cells, interference with BORC triggers LE/Lys trafficking to the cell center via dynein, resulting in a characteristic clustering of LE/Lys in perinuclear regions (Pu et al., 2015). We hypothesized that degradation of Bloc1s1 mRNA by IRE1α during Brucella infection interferes with BORC assembly, resulting in the alteration of recruitment or disassociation of BORC-related trafficking components, and increased LAMP1+-BCV perinuclear trafficking and fusion with the ER and/or macroautophagosome membranes. To test this hypothesis, we performed protein co-immunoprecipitation (Co-IP) assays to measure the association of BORC components with each other in Brucella-infected or -uninfected host cells. We found that in uninfected cells, BLOS1 interacted with protein components of BLOC-1 (PALLIDIN), BORC (KXD1), and both BLOC-1 and BORC (BLOS2, SNAPIN) (Figure 7A, B). Moreover, under this condition, BORC components localized with peripheral or cytosolic LE/Lys membranes (Figure 5—figure supplement 1; Figure 6—figure supplement 1). However, in Brucella-infected cells, where Bloc1s1 mRNA was degraded and BLOS1 protein depletion was observed (Figure 3D, H; Figure 4G, H; Figure 3—figure supplement 3A; Figure 4—figure supplement 1D, E), physical interaction between BLOS1 and BORC component SNAPIN was reduced in control cells and difficult to detect in the mBloc1s1 variants during intracellular trafficking and replication of the pathogen (48 h.p.i.) (Figure 7C, D). In fact, interactions between LYSPERSIN and KXD1 in control cells were also only detected at early time points (2 h.p.i.), but not at later time points corresponding to intervals when bacterial intracellular trafficking and replication were expected to occur; these interactions were also hardly detected in infected cells expressing mBloc1s1 variants at these time points (Figure 7C, E). The reduced interactions between BLOS1 and BORC component SNAPIN as well as LYSPERSIN and KXD1 may result from the disassembly of BORC when BLOS1 is degraded during Brucella infection (Figure 7C–E). In Brucella-infected Bloc1s1 overexpressing (wt-Bloc1s1) cells, substantial reductions in the interactions were also observed (Figure 7F, G). In the infected Rr-Bloc1s1 cells, the interaction was maintained at a relatively higher level (Figure 7F, G), suggesting that the BORC complex remains assembled. The integrity or disassociation of BORC was consistent with the interactions between BORC-related trafficking components and with the colocalization dynamics of BCVs with BLOS1, mTORC1/LAMP1, LAMP1/ARL8b, LAMP1/KIF1b or with LAMP1/KIF5b. These findings were also consistent with BCV peripheral or perinuclear/ER trafficking and accumulation (Figures 5 and 6; Figure 4—figure supplement 3E, F). The results collectively suggested that the degradation of Bloc1s1 mRNA during pathogen infection resulted in the disassembly of BORC, which promoted BCV trafficking to and accumulation in the vicinities of nuclei and likely facilitated the fusion of BCVs with ER membranes in which bacteria replicated.
Host RIDD activity on BLOS1 promotes coronavirus intracellular replication
In light of the global COVID-19 pandemic, we tested whether RIDD-controlled BLOS1 activity is a target for subversion by coronaviruses. We infected control or host cells harboring alterations in this pathway with mouse hepatitis virus [MHV, a positive-strand RNA virus classified as a member of the Betacoronavirus genus (CoV)]. Notably, previous studies have shown that MHV infection induces host cell UPR and activates IRE1α RNase and Xbp1 splicing (Bechill et al., 2008), thereby suggesting the hypothesis that MHV infection of host cells activates RIDD activity. To test this hypothesis, mBloc1s1 or control host cells were untreated or treated with 4μ8C. Next, these cells were infected with MHV for 24 hr. Virus plaque-forming units (PFUs) and host Bloc1s1 expression were then measured. We found that viral PFUs were reduced in 4μ8C-treated cells. However, significantly increased PFU in mBloc1s1 cells at 24 h.p.i. compared to controls was observed (Figure 7H). Expression levels of Bloc1s1 mRNA were dramatically reduced during infection (Figure 7I). Collectively, these findings suggested that coronavirus MHV, like Brucella, subverts the host RIDD pathway to promote intracellular infection.
Discussion
RIDD, a fundamental component of UPR in eukaryotic cells, cleaves a cohort of mRNAs encoding polypeptides that influence ER stress, thereby supporting the maintenance of ER homeostasis. In this report, we found that Brucella infection subverts UPR, in general (Pandey et al., 2018; Qin et al., 2008; Smith et al., 2013; Taguchi et al., 2015), and RIDD activity on Bloc1s1, in particular, to promote intracellular parasitism. BLOS1, encoded by RIDD gene Bloc1s1, is a shared subunit of both BLOC-1 and BORC complexes (Pu et al., 2015). Mutation or a reduction in BLOS1 expression affects both BLOC-1 and BORC (Figure 7A–H). The BLOC-1 complex is mainly involved in endosomal maturation and endosome–lysosome trafficking and fusion (John Peter et al., 2013; Pu et al., 2015; Scott et al., 2018). Our work does not rule out the possibility that the disassociation of BLOC-1 also affects Brucella intracellular parasitism, especially in the early stages of cellular infection.
Investigation of Brucella-mediated RIDD genes provides an avenue for understanding how the pathogen subverts or evades host functions to promote its intracellular lifestyle. For example, Brucella infection downregulates the expression of Txnip, a gene shown in this work to be subject to RIDD control and known to facilitate the intracellular survival of the pathogen (Hu et al., 2020). Cells undergoing ER stress following treatment with UPR inducers (e.g., thapsingargin, Tm) increase TXNIP protein levels and mRNA stability by reducing levels of the TXNIP destabilizing microRNA, miR-17 (Lerner et al., 2012). Therefore, RIDD might be expected to increase the stability of TXNIP mRNA by attacking the miR-17 that affects TXNIP mRNA stability. However, a recent report demonstrated that in vitro cultivated host cells or mouse tissues infected by BaS2308 display reduced expression levels of TXNIP mRNA and/or protein as well as miR-17 (Hu et al., 2020). Consistent with these results, we found that both TXNIP mRNA and miR-17 were destabilized during Brucella infection. These findings suggest that the downregulation of TXNIP during Brucella infection is miR-17 independent. The functions of other RIDD genes and the mechanisms by which they regulate miRNAs and their targets during Brucella infection constitutes an interesting area for further investigation.
Our findings support a stepwise working model by which Brucella subverts the host RIDD pathway to facilitate intracellular parasitism by disrupting BORC-directed lysosomal trafficking (Figure 7J).
First, Brucella enters host cell via the endocytic pathway (Figure 7J, right portion). Bacterial infection also induces UPR in host cells, a process associated with activation of IRE1α kinase (Pandey et al., 2018; Taguchi et al., 2015) and RNase activities (Smith et al., 2013; this work) (Figure 7J, step 1). Second, degradation of the RIDD target Bloc1s1 by IRE1α RNase activity results in depletion of BLOS1 proteins and reduced association with BORC components (Figure 3D and Figure 4G, H; Figure 7A–H; Figure 7J, steps 2 and 3). Third, these events drive the trafficking of BCVs to the ER and their perinuclear accumulation (Figure 7J, steps 4 and 5), mitigate further fusion of BCVs with cytosolic lysosomes, and limit BCV trafficking to LE/Lys in peripheral regions where these organelles possess enhanced degradative functions (Figures 5 and 6; Figure 4—figure supplement 3E, F; Figure 7J, dash-blue line). Finally, the accumulation of BCVs decorated with ER proteins increases due to the fusion of BCVs with ER membranes and/or with noncanonical macrophagosomes (Pandey et al., 2018; Starr et al., 2012; Taguchi et al., 2015; Figure 7J, steps 5 and 6). These final events support the intracellular replication of the pathogen (Figure 7J).
Several lines of evidence support the proposed mechanism. First, Brucella infection activates IRE1α RNase activity as evidenced by Xbp1 mRNA splicing (Figure 3—figure supplement 1A). However, the intracellular replication of the pathogen is not impaired in Xbp1 KO cells and mice (Figure 1N–P). These findings support the hypothesis that IRE1α RNase activity is required for Brucella infection in an IRE1α-XBP1 independent fashion. Second, in addition to splicing Xbp1, IRE1α cleaves other mRNAs, resulting in their RIDD-mediated decay (Bae et al., 2019). We identified several mRNAs, including Bloc1s1 (Figure 3A–C), that contain predicted stem-loop structures that were inferred to be targets of IRE1α RNase activity (Moore and Hollien, 2015). The expression of these mRNAs was downregulated in response to Brucella infection. Host cells harboring nonfunctional Bloc1s1 mutants were highly susceptible to pathogen infection, whereas cells that express a RIDD-resistant Bloc1s1 variant were resistant to Brucella infection (Figure 4E, F).
Third, lysosome positioning regulated by BORC is a critical determinant of its functions. BORC associates peripherally with lysosomal membranes, where it recruits the small GTPase ARL8b to lysosomes. BORC and ARL8b promote lysosome movement by coupling to kinesin-1 (KIF5b) or kinesin-3 (KIF1b), which preferentially moves lysosomes on perinuclear tracks enriched in acetylated α-tubulin or on peripheral tracks enriched in tyrosinated α-tubulin, respectively (Guardia et al., 2016; Pu et al., 2015). Interference with BORC or other components of this pathway drives lysosome trafficking to the cell center via dynein. Thus, cells lacking BORC display a perinuclear clustering of lysosomes (Pu et al., 2015). Ragulator (a GEF for the Rag GTPases that signal amino acid levels to mTORC1) directly interacts with and inhibits BORC functions (Pu et al., 2017). Building upon these observations, we show that Brucella infection results in Bloc1s1 degradation and disassembly of BORC; moreover, during Brucella intracellular trafficking and replication, colocalization of mTORC1, ARL8b, and KIF1b with BCVs or lysosomes was reduced in control cells, and in cells expressing nonfunctional variants of BLOS1; however, KIF5b localization with BCVs or lysosomes was increased or in a higher level in these cells (Figure 5, Figure 5—figure supplement 1; Figure 6, Figure 6—figure supplement 1). Colocalization of the BORC-related lysosome trafficking factors (i.e., ARL8b, KIF1b, and mTORC1) with BCVs or lysosomes in cells expressing Rr-Bloc1s1 variants were maintained at a relatively higher level (Figures 5 and 6). These findings demonstrate that blocking BORC function via the disassembly of the BORC complex through depletion of Bloc1s1 by Brucella infection drives BCVs toward the perinuclear region and ER accumulation, which likely facilitates the fusion of BCVs with the ER, thereby supporting intracellular parasitism.
Finally, RIDD-mediated Bloc1s1 degradation may promote BCV fusion with autophagosomes. Nutrient-starved cells display perinuclear clustering of lysosomes, which influences autophagosome formation and autophagosome–lysosome fusion rates (Korolchuk et al., 2011). Lysosome perinuclear clustering during starvation, ER stress induced by accumulation of misfolded proteins, drug treatments, and pathogen infection can disrupt metabolic homeostasis, thereby necessitating the induction of cell biological processes that return the cell to equilibrium. Macroautophagy and degradation of sequestered cytosolic materials by fusion of autophagosomes/macrophagosomes with lysosomes can promote the re-establishment of homeostasis (Bae et al., 2019; Korolchuk et al., 2011; Pu et al., 2017). Degradation of Bloc1s1 mRNA by IRE1α leads to the perinuclear accumulation of LE/Lys in response to ER stress in mouse cells. Overriding Bloc1s1 degradation results in ER stress sensitivity and the aggregation of ubiquitinated proteins. The LE/Lys perinuclear-trafficking and LE-associated endocytic transport promote the efficient degradation of these protein aggregates. Therefore, Bloc1s1 regulation via RIDD facilitates LE-mediated autophagy of protein aggregates, thereby promoting cell survival during stress (Bae et al., 2019). Hepatocytes from Bloc1s1 liver-specific knockout (DelVecchio et al., 2002) cells accumulate autolysosomes and lysosomes. In LKO hepatocytes, the initiation or extension of lysosomal tubules is abolished, which impairs autophagic lysosome reformation and results in the accumulation of enlarged autolysosomes (Wu et al., 2021b). Bloc1s1 degradation by the RIDD pathway promotes BCV perinuclear or ER-region clustering and may also avoid the peripheral movement of BCVs away from the ER region as a consequence of reduced α-tubulin acetylation. These processes may facilitate BCV fusion with ER membranes or (macro)phagosomes, promote the enlargement of aBCVs and further bacterial replication, and ultimately relieve Brucella-induced ER stress (Pandey et al., 2018; Qin et al., 2008; Starr et al., 2012; Taguchi et al., 2015).
In addition to Brucella, the betacoronavirus MHV also subverts the RIDD–BLOS1 axis to promote intracellular replication (Figure 7H, I), thereby indicating that RIDD control of BLOS1 activity is not pathogen specific. How the host RIDD–Bloc1s1 axis regulates interactions between host cells and coronaviruses merits further investigation. However, additional possibilities for regulatory control can be envisioned. First, coronaviruses utilize many proteins such as nsp1 to inhibit host protein synthesis in the first 6 hr of infection (Nakagawa and Makino, 2021). Second, BLOS1 contains a potential coronavirus 3C-like protease cleavage site, LQ^SAPS, near its C-terminus, thereby rendering it potentially susceptible to direct subversion by coronaviral pathogens. Finally, coronaviruses have evolved to subvert host interferon defenses (Thoms et al., 2020), which may contribute to immune evasion. Future work will be directed toward examining these possibilities and the roles and mechanisms by which the RIDD-Bloc1s1 axis controls these and other host–pathogen interactions.
Materials and methods
All the key resources used in the work are listed in Key resources table used in this work.
Bacterial strains, cell culture, Brucella infection, and antibiotic protection assays
Request a detailed protocolBrucella melitensis strain 16M (WT), and B. abortus strain 2308 (WT), and B. abortus vaccine strain S19 and other bacterial strains were used in this work. Bacteria were grown in tryptic soy broth (TSB) or on tryptic soy agar (TSA, Difco) plates, supplemented with either kanamycin (Km, 50 μg/ml) or chloramphenicol (Cm, 25 μg/ml) when required. For infection, 4 ml of TSB was inoculated with a loop of bacteria taken from a single colony grown on a freshly streaked TSA plate. Cultures were then grown with shaking at 37°C overnight, or until OD600≈ 3.0.
Mammalian host cells including murine macrophages RAW264.7 and its derived nonfunctional and Rr-Bloc1s1 variants and corresponding control cells, BMDMs, J774.A1 cells, and MEFs were routinely cultured at 37°C in a 5% CO2 atmosphere in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and containing 1% penicillin/streptomycin to prevent mycoplasma contamination. Murine osteoblasts MC3T3-E1 and its derived Rr-Bloc1s1 variant and corresponding control cells (Bae et al., 2019) (generously provided by the Hollien Lab) were routinely cultured at 37°C in a 5% CO2 atmosphere in alpha minimum essential media with nucleosides, L-glutamine, and no ascorbic acids, supplemented with 10% FBS. Murine fibroblasts L2 cells were routinely cultured at 37°C in a 5% CO2 atmosphere in F12 medium supplemented with 10% fetal calf serum (FCS). For BMDMs, the abovementioned DMEM with 20% L929 cell supernatant, 10% FBS, and antibiotics was used. Cells were seeded in 24- or 96-well plates and cultured overnight before infection. For antibiotic protection assays, 1.25 × 105 (BMDMs) or 2.5 × 105 (RAW264.7) host cells were seeded in each well; for fluorescence microscopy assays, 1 × 104 or 5 × 104 cells were seeded in 96-well plates or on 12 mm glass coverslips (Fisherbrand) placed on the bottom of 24-well microtiter plates, respectively; for host RNA analysis, 1 × 105 host cells were seeded in each well of 24-well plates before infection. Host cells were infected with Brucella at an MOI of 100, unless otherwise indicated. Infected cells were then centrifugated for 5 min (200 × g) and incubated at 37°C. Thirty minutes to 1 hr postinfection, culture media was removed, and the cells were rinsed with 1× phosphate buffered saline (PBS, pH 7.4). Fresh media supplemented with 50 μg/ml gentamicin was then added for 1 hr to kill extracellular bacteria. Infected cells were continuously incubated in the antibiotic. At the indicated time points post infection, viable bacteria in infected cells were analyzed using the antibiotic protection assay or the immunofluorescence microscopy assay as previously described (Pandey et al., 2018; Qin et al., 2008).
Viral propagation, infection, and plaque assay
Request a detailed protocolWild type MHV-A59 was propagated in L2 cells in F12 media with 2% FCS. Host cells (RAW 264.7) were infected with MHV-A59 in triplicate at a MOI of 1. Infected cells were incubated at room temperature with gentle rocking for 1 hr. Afterwards, culture media was removed, and the cells were rinsed with 1× PBS (pH 7.4). Fresh media supplemented with 2% FBS was added. Infected host cells were incubated at 37°C. At the indicated time points post infection, viral supernatants were collected and then titrated by plaque assay on L2 cells at 33°C.
Generation, genotyping, and characterization of Ern1 CKO mice
Request a detailed protocolAnimal research was conducted under the auspices of approval by the Texas A&M University Institutional Animal Care and Use Committee in an Association for Assessment and Accreditation of Laboratory Animal Care International Accredited Animal Facility. To investigate the roles of IRE1α in controlling Bm16M intracellular parasitism, Ern1 CKO (Ern1flox/flox; Lyz2-Cre) mice were generated by crossing Ern1-floxed mice, in which exons 20 and 21 of Ern1 were floxed, with Lyz2-Cre mice carrying the Cre recombinase inserted in the Lysozyme M (Lyz2) gene locus. In the resultant animals (gift from the Iwawaki lab), exons 20 and 21 of the Ern1 gene were specifically deleted in myeloid cells, including macrophages, monocytes, and neutrophils. The Ern1 CKO mice were genotyped using genomic DNA from tail vain to show the presence of Cre alleles (Iwawaki et al., 2009). Western blot analysis using anti-IRE1α antibodies (Novus Biologicals) and Xbp1 splicing were performed on BMDMs from CKO and control mice to validate the absence of full-length IRE1α in CKO mice.
BMDM harvest and cultivation
Request a detailed protocolBMDMs collected from the femurs of Ern1 CKO and control mice were cultivated in L929-cell conditioned media [DMEM medium containing 20% L929 cell supernatant, 10% (vol/vol) FBS, penicillin (100 U/ml) and streptomycin (100 U/ml)]. After 3 days of culture, nonadherent precursors were washed away, and the retained cells were propagated in fresh L929-cell conditioned media for another 4 days. BMDMs were split in 24-well plates (2.5 × 105 cells/well) in L929-cell conditioned media and cultured at 37°C with 5% CO2 overnight before use.
Whole animal infections with Brucella and histologic analysis
Request a detailed protocolMice from CKO and littermate control groups (5 mice/group or treatment) were intraperitoneally infected with B. melitensis and B. abortus (Bm16M and BaS2038, respectively) with a dose of 1 × 106 CFU. At 7 and 14 dpi, infected mice were euthanized, and the bacterial burden was assessed in spleen and liver. A portion of the tissue was fixed, and paraffin embedded for histopathological examination following hematoxylin and eosin staining. Sections were evaluated and scored for lesion severity (inflammation) using the previously described scoring system (Lacey et al., 2018), that is, 0 = no inflammation; 1 = minimal with inflammation involving <5% of tissue; 2 = moderate with focally extensive areas of inflammation (5–25% of tissue and involving 1 or more tissues); 3 = moderate to severe with focally extensive areas of inflammation (>25% to 50% of tissue and involving multiple tissues); and 4 = severe with large confluent areas of inflammation (>50% of tissue and involving multiple tissues). To assess Bm16M tissue burden, spleen or liver tissues were homogenized and subjected to a serial dilution. Finally, the diluted tissue homogenates (200 μl) were plated on TSA solid plates and CFUs were determined at 48- to 60-hr postincubation at 37°C in 5% CO2.
Latex bead phagocytosis assays
Request a detailed protocolPhagocytosis assays for testing the phagocytic uptake and route of a substrate in the nonfunctional and RIDD-resistant Bloc1s1 variants in RAW264.7 murine macrophages were performed using the Phagocytosis Assay Kit (IgG FITC) (Cayman Chemical, USA) according to the manufacturer’s instructions.
RNA extraction and qRT-PCR analysis
Request a detailed protocolRNA was extracted from host cells per instructions in the RNeasy Mini Kit (74,134 Qiagen). Complementary DNA was amplified from mRNA using the High-Capacity cDNA Reverse Transcription Kit (4368813 Applied Biosystems) per manufacturer’s guidelines. For qRT-PCR of the macrophage infections of MHV strain A59, BaS19, Bm16M, or Ba2308 1/5 dilution of each cDNA was added into nuclease free water in a respective well in a 96-well plate. SYBR green (50–90 μl) with primers (5–9 μl) were put into triplicate wells of each respective primer in the same respective 96-well plate for all experiments. Primers for Bloc1s1, Cd300If, Diras2, Txnip, miR-17-5p, U6, and Gapdh were used (Supplementary file 2). The cDNA and master mix were transferred to a 384-well plate using E1 ClipTip pipettor (4672040 Thermo Scientific). The qPR-PCR was run on a CFX384 Real-Time System (BioRad).
RNA-seq analysis
Request a detailed protocolAll RNA-seq reads were mapped to Mus musculus reference genome GRCm38.p4, release 84, which is provided by Ensembl.org, by using STAR-2.5.2a (Dobin et al., 2013). The aligned reads were then counted by using RSEM-1.2.29 (Li and Dewey, 2011). Differential Expression Analysis was performed by using DESeq-1.30.0 (Anders and Huber, 2010) and edgeR-3.26.6 (McCarthy et al., 2012; Robinson et al., 2010). Differentially expressed gene (DEGs) were determined if the gene’s p value (significance of differential expression) <0.05 and the absolute value of the fold change >1.5.
Bioinformatic analysis
Request a detailed protocolKEGG pathway enrichment analysis was performed by using WebGestalt-2013 (WEB-based Gene Set Analysis Toolkit) (Wang et al., 2013) with RIDD genes. Gene Ontology (GO) Enrichment was performed by feeding the significantly DEGs to PANTHER-v7 (Gaudet et al., 2011; Mi et al., 2010) in all three classes: Molecular Function, Biological Process, and Cellular Component. Both KEGG pathway and GO enrichment analysis were filtered and sorted by Fisher test and Benjamini and Hochberg adjustment. The GeneCards website (Stelzer et al., 2016) was used to connect to the REFSEQ mRNAs from NCBI’s GenBank. The FASTA option was chosen and the fasta file was saved. The fasta file was uploaded to RNAfold Web Server (Institute for Theoretical Chemistry at University of Vienna) with the fold algorithm options of minimum free energy and partition function and avoid isolated base pairs. The Forna option was then used to view the secondary structure and manually searched for a stem loop with NNNNNCNGNNGNNNNNN. Interaction network analysis of KEGG pathways and the RIDD genes (p < 0.05) identified in this work was visualized using Cytoscape (https://cytoscape.org/) as described previously (Zhao et al., 2017).
Generation of nonfunctional and RIDD-resistant Bloc1s1 variants in RAW264.7 murine macrophages
Request a detailed protocolNonfunctional Bloc1s1 variants in RAW264.7 murine macrophages were generated using a protocol previously described (Hoffpauir et al., 2020). One clone containing either an amino acid deletion substitution or deletion in one of the XAT regions of murine BLOS1 (Figure 4—figure supplement 1A) was selected. For generation of Rr-Bloc1s1 variant and its control (wt-Bloc1s1) in RAW264.7 murine macrophages, RNA was first extracted using RNeasy Plus mini-kit. Bloc1s1 cDNA was generated from mRNA and cloned into pCR 2.1-TOPO vector. Site-directed mutagenesis was utilized to generate a g449t mutation into one of the Bloc1s1 plasmid clones (Figure 4—figure supplement 1B). Both the wild-type (WT) and mutated Bloc1s1 segments were removed from the pCR 2.1-TOPO vector and cloned into pE2n vectors. Gateway cloning was used to generate expression vectors pLenti-CMV-Hygro-DEST (w117-1)-Blos1-WT and pLenti-CMV-Hygro-DEST (w117-1)-Blos1-G449T. At every step post cDNA creation, plasmid amplification vectors were verified via sequencing. The plasmids were then transfected into Lenti-X cells with psPAX2 and VSVG packaging plasmids. The virus was collected at 24- and 48-hr post-transfection and stored at −80°C. RAW264.7 cells were transduced with the virus and cells containing the wt- or Rr-Bloc1s1 expression cassette were selected using hygromycin B (500 μg/ml).
Drug treatments
Request a detailed protocolHost cells were coincubated in 24-well plates with tunicamycin or 4μ8C at the indicated concentrations. Cells were treated with drugs 1 hr before, and during, infection with the indicated Brucella strains and incubated at 37°C with 5% CO2. At the indicated time points postinfection, the treated cells were fixed with 4% formaldehyde and stained for immunofluorescence analysis or lysed to perform CFU assay or for RNA extraction assays as described above. To investigate whether the drugs inhibit Brucella growth, the drugs were individually added to Brucella TSB cultures at 37°C and incubated for 1 and 72 hr. CFU plating was used to assess bacterial growth in the presence of drugs, and thereby to evaluate the potential inhibitory effects. Host cells in which drug treatment or Brucella infection induced no significant differences in viability and membrane permeability as well as drugs that have no adversary effect on Brucella growth were used in the experiments reported in this work.
CIM assays
Request a detailed protocolImmunofluorescence microscopy staining and imaging methods to determine Brucella intracellular trafficking and colocalization of the bacteria and host BORC components in infected host cells were perform as previously described (Pandey et al., 2017; Pandey et al., 2018; Qin et al., 2011; Qin et al., 2008) with minor modifications. Briefly, to visualize Bm16M intracellular trafficking, the indicated host cells (5.0 × 104 for 24-well plates and 1 × 104 for 96-well plates) were seeded on 12 mm coverslips placed on the bottom of wells of 24- or 96-well plates (without coverslip) and infected with Bm16M-GFP or Bm16M. At 0.5 (for 24-well plates) or 1 (for 96-well plates) h.p.i., the infected cells were washed with 1× PBS and fresh media containing 50 μg/ml gentamicin was added to kill extracellular bacteria. At the indicated time points post infection, the infected cells were fixed with 4% formaldehyde at 4°C for overnight or for 20 min at 37°C before confocal fluorescence or immunofluorescence microscopy analysis was performed as previously described (Pandey et al., 2017; Pandey et al., 2018; Qin et al., 2011; Qin et al., 2008). The primary antibodies used were listed in the Key Resources Table. Samples were stained with Alexa Fluor 488-conjugated, Alexa Fluor 594-conjugated, and/or Alexa Fluor 647 secondary antibody (Invitrogen/Molecular Probes, 1:1000). Acquisition of confocal images, and image processing and analyses were performed as previously described (Pandey et al., 2017; Pandey et al., 2018; Qin et al., 2011; Qin et al., 2008).
The BioTek Cytation 5 and Gen 5 software (version 3.05) were used to calculate perinuclear Lamp1 index and autophagic flux. Specifically, for perinuclear Lamp1 index, the area of the nucleus and average intensity for Lamp1 was measured, noted as AN and IntN, respectively. The area of the whole cell and the average intensity for Lamp1 was measured, noted as AWC and IntWC, respectively. Next the area of the cell 3 µm off from the nucleus (peripheral region) and the average intensity for Lamp1 was measured, noted as AC and IntC, respectively. Then, IntWCN (average intensity of Lamp1 in the whole cell minus nucleus) and IntP (average intensity of Lamp1 in the perinuclear region) were calculated as the following formula:
If IntP/IntWCN ≤ 1, which means that there is no or very little perinuclear colocalization; if IntP/IntWCN > 1, which means that there is perinuclear colocalization.
For autophagic activity, using BioTek Cytation 5 and Gen 5 software (version 3.05), the mean intensity of LC3b in the nucleus (M1) and in the cytoplasm (M2) were measured using a primary and secondary mask in every individual cell. In the Gen 5 software, a subpopulation analysis was carried out to identify cells that had a ratio of M2/M1 <1. From this, the perinuclear LC3b index or autophagic activity was calculated as the following formula: Perinuclear LC3b index (or autophagic activity) = (Total number of identified cells with M2/M1 <1)/Total number of the analyzed cells.
For calculation of the relative BLOS1 fluorescence integrated density (FID) per BCV, the corrected total cell fluorescence was measured by taking the integrated density (area of cells × mean fluorescence), then the number of Brucella in each cell and colocalization of BLOS1 and Bm16M (BCV-BLOS1+, %) were counted. Cells that did not contain bacteria were removed from the calculation. The integrated density was divided by the number of bacteria in the cell to obtain the ‘Total fluorescence per bacteria’. The ‘relative BLOS1 FID per BCV’ was then calculated by multiplying the ‘Total fluorescence per bacteria’ with the colocalization percentage. Since BLOS1 protein is more stable in control cells treated with 4μ8C, the value of the relative BLOS1 FID per BCV was normalized as 1.
Protein pull-down assays and immunoblotting analysis
Request a detailed protocolPull-down assays for testing physical interaction of proteins were perform using the Pierce Co-Immunoprecipitation (Co-IP) Kit (Thermo Scientific, USA) according to the manufacturer’s instructions. Preparation of protein samples and immunoblotting blot analysis were performed as described previously (Ding et al., 2021; Pandey et al., 2017; Pandey et al., 2018; Qin et al., 2011). Densitometry of blots was performed using the ImageJ (http://rsbweb.nih.gov/ij/) software package. All Westerns were performed in triplicate and representative findings are shown.
Statistical analysis
Request a detailed protocolAll the quantitative data represent the mean ± standard error of mean from at least three biologically independent experiments, unless otherwise indicated. The data from controls were normalized as 1% or 100% to easily compare results from different independent experiments. The significance of the data was assessed using the Student’s t-test (for two experimental groups), two-way analysis of variance test with Holm–Sidak’s multiple comparisons, or the Kruskal–Wallis test with Dunn’s multiple comparison. For the RNA-seq results, Log2 fold changes were calculated and results were screened to meet the threshold (|log2FC (fold change)| > 1, p < 0.05) for selection. DEGs met the criteria and in UPRsome were included in the final lists.
Key resources
Request a detailed protocolAll key resources, including bacterial strains, mammalian cell lines, reagents, etc. used in this work are listed in Key resource table.
Data availability
All data generated or analyzed during this study are included in the manuscript, supporting file, and source data file.
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Article and author information
Author details
Funding
Texas A and M University (Clinical Science Translational Research Institute Pilot Grant CSTR2016-1)
- Paul de Figueiredo
Defense Advanced Research Projects Agency (HR001118A0025-FoF-FP-006)
- Paul de Figueiredo
National Institutes of Health (R21AI139738-01A1)
- Paul de Figueiredo
National Institutes of Health (1R01AI141607-01A1)
- Paul de Figueiredo
National Institutes of Health (1R21GM132705-01)
- Paul de Figueiredo
National Science Foundation (DBI1532188)
- Paul de Figueiredo
National Science Foundation (NSF0854684)
- Paul de Figueiredo
Bill and Melinda Gates Foundation
- Paul de Figueiredo
National Natural Science Foundation of China (81371773)
- Qing-Ming Qin
National Institutes of Health (RHD084339)
- Thomas A Ficht
The funders had no role in study design, data collection, and interpretation, or the decision to submit the work for publication.
Acknowledgements
The authors are grateful to Drs. Takao Iwawaki (Kanazawa Medical University, Japan), Laurie H Glimcher (Cornell University, USA), R J Kaufman (Howard Hughes medical Institute at University of Michigan Medical School, USA), Julie Hollien (University of Utah, USA) for sharing Ern1 CKO and control mice, Xbp1 CKO and control mice, Ern1+/+ and Ern1−/− MEF cells, and Bloc1s1− expressing and control cell lines, respectively, used in this study; to Drs. Christine McFarland, Jessica Bourquin, Todd Wisner, and Susan Gater (Texas A&M) for key support; and to Drs. Steve Fullwood and Kalika Landua (Nikon Instruments) for expert assistance with the microscopy analysis. This work is supported by the Texas A&M Clinical Science Translational Research Institute Pilot Grant CSTR2016-1, the Defense Advanced Research Projects Agency (DARPA) (HR001118A0025-FoF-FP-006), NIH (R21AI139738-01A1, 1R01AI141607-01A1, 1R21GM132705-01), the National Science Foundation (DBI 1532188, NSF0854684), the Bill Melinda Gates Foundation, and to PdF; the National Natural Science Foundation of China (# 81371773) to QMQ. The National Institute of Child Health and Human Development [RHD084339] to TAF (in part). The National Science Foundation Grant 1553281 to XQ. The content of the information does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred.
Ethics
LysM-Ern1mut/mut or Xbp1-KO and control mice were generated under the auspices of approval by the Texas A & M University Institutional Animal Care and Use Committee in an Association for Assessment and Accreditation of Laboratory Animal Care International Accredited Animal Facility.
Copyright
© 2022, Wells et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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