4 figures, 1 video and 5 additional files

Figures

Figure 1 with 4 supplements
ZAF as a simple solution for aquatic facility feeding.

(a) Schematic representation of ZAF’s three main modules with their key components. Basic electronic wiring is also shown. ZAF is designed to distribute the same food quantity to all (fish) tanks. (b

Figure 1—figure supplement 1
Automatising Zebrafish husbandry.

(a) Schematic representation of the standalone single rack zebrafish facility where we tested ZAFs. The facility is semi automatic and does not require constant human supervision. (b) Outside …

Figure 1—figure supplement 2
ZAF electronic diagram.

The design is based on a Raspberry computer, a Servo Hat Board to drive Pulse Width Modulation outputs and motor controller to control the pumps and valve. All the pumps and valves connected to the …

Figure 1—figure supplement 3
ZAF tubing.

In this schematic graph, we indicate how to connect the different pumps and valves together and where to the different types of tubing.

Figure 1—figure supplement 4
Water parameters log, pH and conductivity, during ZAF testing over a 3- month period.
Figure 2 with 4 supplements
ZAF+ is an advanced version of ZAF that can modulate food delivery per tank depending on fish density.

(a) Diagram of ZAF+. Electronics consist of: a Raspberry Pi 3, Arduino Mega, 16 relay module, an motor controllers. The water and food mix is pumped and sent via tubes to a manifold and valves that …

Figure 2—figure supplement 1
ZAF+ electronics diagram.

ZAF+ main electronics components are a Raspberry Pi 3B+ to run the software and control the electronics, Arduino Megas Arduino 2560 microcontrollers for the digital devices, motor controllers to …

Figure 2—figure supplement 2
ZAF+ tubing.

The following schematic diagram represents the tubing connections between the different ZAF+ components. In order to connect the pump to multiple valves (30, in our case), we used manifolds that …

Figure 2—figure supplement 3
Tubing cleanliness evaluation.

In our fish facility conditions we observed that the tubes should be replaced every 12 weeks .

Figure 2—figure supplement 4
Water parameters log, pH and conductivity, during ZAF+ testing over a 3- month period.
Box 1—figure 1
Diagram illustrating the differences during ZAF (left) and ZAF+ (right). ZAF is designed to distribute an homogeneous mix of food for all the tanks, whereas ZAF+ can control the amount of food per tanks.
Box 2—figure 1
ZAF+ Graphic User Interface main panels. On the top panel ‘Dashboard’ displays the overview of the entire program. Bottom part illustrates the ‘Program tab’, where you set all the conditions for feeding and washing.

Videos

Video 1
ZAF presentation video, introducing the main features of the automatic feeders.

Additional files

Supplementary file 1

Detailed construction instruction for ZAF and ZAF+.

https://cdn.elifesciences.org/articles/74234/elife-74234-supp1-v2.pdf
Supplementary file 2

ZAF+ parts list.

The table lists the necessary parts to build ZAF. Most of the parts are generic and can be replaced by components with similar specifications.

https://cdn.elifesciences.org/articles/74234/elife-74234-supp2-v2.docx
Supplementary file 3

ZAF+ parts list.

The table lists the necessary parts to build ZAF+. Most of the parts are generic and can be replaced by components with similar specifications.

https://cdn.elifesciences.org/articles/74234/elife-74234-supp3-v2.docx
Supplementary file 4

Troubleshooting guide.

This table provides solutions to common minor issues encountered during ZAFs construction and operations.

https://cdn.elifesciences.org/articles/74234/elife-74234-supp4-v2.docx
Transparent reporting form
https://cdn.elifesciences.org/articles/74234/elife-74234-transrepform1-v2.pdf

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