strain, strain background (Escherichia Coli) | One shot top10 | Invitrogen | Cat#: C404010 | |
genetic reagent (D. melanogaster) | y[1] w[*]; Mi{y[+mDint2]= MIC}Syt1[MI02197] | Bloomington Drosophila Stock CenterPMID: 21985007 | BDSC:35973FLYB: FBal0314405 RRID:BDSC_35973 | FlyBase symbol: Mi{y[+mDint2]= MIC}Syt1[MI02197] |
genetic reagent (D. melanogaster) | y[1] w[*] Mi{y[+mDint2]= MIC}Sh[MI10885] | Bloomington Drosophila Stock CenterPMID: 21985007 | BDSC:56260FLYB: FBal0297530 RRID:BDSC_56260 | FlyBase symbol: Mi{MIC}ShMI10885 |
genetic reagent (D. melanogaster) | y[1] w[*];Mi{y[+mDint2]= MIC}Shal[MI10881] | Bloomington Drosophila Stock CenterPMID: 21985007 | BDSC:56089FLYB: FBal0295200 RRID:BDSC_56089 | FlyBase symbol: Mi{MIC}ShalMI10881 |
genetic reagent (D. melanogaster) | y[1] w[*]; Mi{y[+mDint2]=MIC} Shab[MI00848] | Bloomington Drosophila Stock CenterPMID: 21985007 | BDSC:34115FLYB: FBal0249123 RRID:BDSC_34115 | FlyBase symbol: Mi{MIC}ShabMI00848 |
genetic reagent (D. melanogaster) | nSyb-GAL4 | Bloomington Drosophila Stock CenterPMID: 18621688 | BDSC: 39171FBgn0013342 RRID:BDSC_39171 | Flybase symbol: P{GMR57C10-GAL4} |
genetic reagent (D. melanogaster) | repo-GAL4 | Bloomington Drosophila Stock Center PMID: 7926782 | BDSC:7415FLYB: FBal0127275 RRID:BDSC_7415 | FlyBase symbol: P{GAL4}repo |
genetic reagent (D. melanogaster) | repo-QF2 | Bloomington Drosophila Stock Centerdoi:10.1534/ genetics.116.191783 | BDSC:66477FLYB: FBal0322908 RRID:BDSC_66477 | FlyBase symbol: P{ET-QF2.GU}repo |
genetic reagent (D. melanogaster) | Shaw-GAL4 | Bloomington Drosophila Stock CenterPMID: 21985007 | BDSC:60325FLYB: FBal0304243 RRID:BDSC_60325 | FlyBase symbol: GAL4Shaw- MI01735-TG4.1 |
genetic reagent (D. melanogaster) | Ddc-GAL4 | Bloomington Drosophila Stock Center doi:10.1006/ dbio.1994.1261 | BDSC:7009FLYB: FBtp0012451 RRID:BDSC_7009 | FlyBase symbol: P{Ddc-GAL4.L} |
genetic reagent (D. melanogaster) | TH-GAL4 | Bloomington Drosophila Stock CenterPMID: 12555273 | BDSC:8848FLYB: FBtp0114847 RRID:BDSC_8848 | FlyBase symbol: P{ple-GAL4.F}3 |
genetic reagent (D. melanogaster) | Trh-GAL4 | Bloomington Drosophila Stock Centerdoi:10.1371/journal. pone.0010806 | BDSC:38389FLYB: FBtp0055412 RRID:BDSC_38389 | FlyBase symbol: P{Trh-GAL4.long} |
genetic reagent (D. melanogaster) | UAS_H2A-GFP | Steve Stowers (Montana SU)doi:10.1371/journal. pgen.1008609 | FLYB: FBgn0001196 | |
genetic reagent (D. melanogaster) | QUAS_H2A-mCherry | Steve Stowers(Montana SU)doi:10.1371/journal. pgen.1008609 | FLYB: FBgn0001196 | |
genetic reagent (D. melanogaster) | brp-GAL4 | This paper, available upon request, https://www.epfl.ch/labs/mccabelab/resources/ | FLYB:FBgn0259246 | See Materials and Methods, Section 2 |
genetic reagent (D. melanogaster) | Syt1-Gal4 | This paper, available upon request, https://www.epfl.ch/labs/mccabelab/resources/ | FLYB: FBal0314405 | See Materials and Methods, Section 4 |
genetic reagent (D. melanogaster) | Sh-Gal4 | This paper, available upon request,https://www.epfl.ch/labs/mccabelab/resources/ | FLYB: FBal0297530 | See Materials and Methods, Section 4 |
genetic reagent (D. melanogaster) | Shal-Gal4 | This paper, available upon request,https://www.epfl.ch/labs/mccabelab/resources/ | FLYB: FBal0249123 | See Materials and Methods, Section 4 |
genetic reagent (D. melanogaster) | Shab-Gal4 | This paper, available upon request,https://www.epfl.ch/labs/mccabelab/resources/ | FLYB: FBal0249123 | See Materials and Methods, Section 4 |
genetic reagent (D. melanogaster) | UAS_H2A::GFP- T2A-mKok::Caax | This paper, available upon request,https://www.epfl.ch/labs/mccabelab/resources/ | FLYB: FBgn0001196 | See Materials and Methods, Section 3 |
antibody | anti-myc9EH10(Mouse monoclonal) | DSHB | DSHB Cat# 9E 10, RRID:AB_2266850 | IF(1:100) |
antibody | anti-GFP(Chicken polyclonal) | Abcam | Cat#ab13970 | IF(1:500) |
antibody | anti-Deadpan(Rat monoclonal) | Abcam | Cat#ab195173 | IF(1:50) |
antibody | Goat Anti-Mouse IgG (H+L), Alexa Fluor 488 (Goat polyclonal Secondary Antibody) | Jackson ImmunoResearch | Cat#115-545-166 | IF(1:400) |
antibody | Goat Anti-Horseradish Peroxidase,Alexa Fluor 647 (Goat polyclonal) | Jackson ImmunoResearch | Cat#123-605-021 | IF(1:200) |
antibody | Goat anti-Chicken IgY (H+L), Alexa Fluor 488 (Goat polyclonal Secondary Antibody) | ThermoFisher | Cat#A-11039 | IF(1:500) |
antibody | Goat anti-Rat IgG (H+L) Cross-Adsorbed, Alexa Fluor 594 (Goat polyclonal Secondary Antibody) | ThermoFisher | Cat#A-11007 | IF(1:500) |
recombinant DNA reagent | pBID_DSCP-G-Gal4 (plasmid) | McCabe Lab,Available upon request.https://www.epfl.ch/labs/mccabelab/resources/ | Cat# #35,200 | |
recombinant DNA reagent | pJFRC81-10XUAS-IVS- Syn21-GFP-p10 (plasmid) | Addgene | Cat#: 36,432 | |
recombinant DNA reagent | pHD-sfGFP Scareless dsRed (plasmid) | Addgene | Cat#: 80,811 | |
recombinant DNA reagent | pCFD3 (plasmid) | Addgene | Cat#: 49,410 | |
recombinant DNA reagent | pCR4 brp-Gal4 | This paper, available upon request, https://www.epfl.ch/labs/mccabelab/resources/ | | CRISPR construct inserted in D. Melanogaster |
recombinant DNA reagent | pBID LexO_H2A-mCherry | Gift from Steve Stowers (Montana SU), DOI: 10.1371/journal.pgen.1008609 | | |
recombinant DNA reagent | pCS2+ChMermaid S188 | Addgene | Cat#: 53,617 | |
recombinant DNA reagent | pBID-UAS_H2A::GFP-T2A-mKok::Caax | This paper, available upon request, https://www.epfl.ch/labs/mccabelab/resources/ | | construct inserted in D. Melanogaster |
sequence-based reagent | pCFD3 gRNA brp-5’ | This paper | Guide RNA for Brp CRISPR knock in | GGTGAACCGA CCGGGACAAC |
sequence-based reagent | pCFD3 gRNA brp-3’ | This paper | Guide RNA for Brp CRISPR knock in | GGGAGCCCCGC GACCGCTCC |
sequence-based reagent | brp Ha1 Fo | This paper | PCR primer | GAGAGAGCATCT CGATTGTGCCGTGTG |
sequence-based reagent | brp Pam7 Re | This paper | PCR primer | AATGTTGTCCCG GTCGGTTCACCG |
sequence-based reagent | brp Pam7_In1 Re | This paper | PCR primer | TTCTAGCGTCCAA CGGCTCAGCTGTG GGCCATTTTCTAGT AATGTTGTCCCGG TCGGTTCACCG |
sequence-based reagent | brp HA_In1 Fo | This paper | PCR primer | ACTAGAAAATGGCC CACAGCTGAGCC |
sequence-based reagent | brp V5_In1 Re | This paper | PCR primer | TAGAATCGAGACCG AGGAGAGGGTTAGGG ATAGGCTTACCCATT GCTGAAATTCACACA CACACAGAATTCATGAG |
sequence-based reagent | brp V5_ Fo | This paper | PCR primer | GGTAAGCCTATCCC TAACCCTCTCCTC |
sequence-based reagent | brp PB5’ Re | This paper | PCR primer | TTAAGGGATCTTTCTA TTAGTATAACACTGCATGC |
sequence-based reagent | brp Ex2 fo | This paper | PCR primer | AAATTGCATGCAGTGTT ATACTAATAGAAAGATCC CTTAATCGGCAGTCCAT ACTACCGCGACATGGATG |
sequence-based reagent | brp Pam2_Re | This paper | PCR primer | TCTGGAGCGGT CGCGGGGC |
sequence-based reagent | brp Pam2_Brp Ha2 Re | This paper | PCR primer | GCTCGTCCTCTAGGTAC AGGCCCCGTTCGAGGGA TCTGTCTCTGGAGC GGTCGCGGGG |
sequence-based reagent | brp Ha2 Fo | This paper | PCR primer | GACAGATCCCTC GAACGGGGCC |
sequence-based reagent | Syn21 H2A Fo | This paper | PCR primer | AACTTAAAAAAAAAA ATCAAAATGTCTGGA CGTGGAAAAGGTGGC |
sequence-based reagent | H2A Re | This paper | PCR primer | CCCAAGAAGACC GAGAAGAAGGCC |
sequence-based reagent | H2A-GFP Fo | This paper | PCR primer | ACAGGCTGTTCTGT TGCCCAAGAAGACC GAGAAGAAGGCCAT GGTGTCCAAGGG CGAGGAG |
sequence-based reagent | GFP-T2A Re | This paper | PCR primer | GGGTTCTCCTCCAC ATCGCCGCAGGTCAG CAGGCTGCCGCGGC CCTCCTTGTACAGCT CATCCATGCCCAGG |
sequence-based reagent | T2A-mKok Fo | This paper | PCR primer | GCGGCAGCCTGCT GACCTGCGGCGATG TGGAGGAGAACCCC GGGCCCATGGTGAGT GTGATTAAACCAG AGATGAAGATG |
sequence-based reagent | mKok-Caax Re | This paper | PCR primer | TTACATAATTACACA CTTTGTCTTTGACTT CTTTTTCTTCTTTTTA CCATCTTTGCTCATGG AATGAGCTACTGCAT CTTCTACCTGC |
chemical compound, drug | Formaldehyde 37% | Sigma | Cat#: 252,549 | |
chemical compound, drug | Low melt agarose | Peq gold | Cat#: 35–2010 | |
chemical compound, drug | VECTASHIELD Antifade Mounting Media | VECTOR Laboratories | Cat#: H-1000 | |
chemical compound, drug | FluoSpheres, 0.2 µm, red fluorescent (580/605) | Thermo Fisher Scientific | Cat#: F8810 | |
commercial assay or kit | Zero Blunt TOPO PCR Cloning Kit | Invitrogen | Cat#: 450,245 | |
commercial assay or kit | pCR8/GW/TOPO TA Cloning Kit | Invitrogen | Cat#: K250020 | |
commercial assay or kit | Gateway LR Clonase II Enzyme mix | Invitrogen | Cat#:11791020 | |
commercial assay or kit | NEBuilder HiFi DNA Assembly Master Mix | New England Biolabs | Cat#: E2621S | |
commercial assay or kit | KLD enzyme mix | New England Biolabs | Cat#:M0554S | |
commercial assay or kit | Platinium Superfi polymerase | Invitrogen | Cat#:12359010 | |
software, algorithm | CNS nuclei distance (code for Matlab) | https://doi.org/10.5281/zenodo | | This manuscript |
software, algorithm | Fiji | https://fiji.sc/ | RRID:SCR_002285 | |
software, algorithm | Arivis Vision4D 3.0.0 | Arivis | RRID:SCR_018000 | |
software, algorithm | MATLAB (R2018a) | MathWorks | RRID:SCR_001622 | |
software, algorithm | GraphPad Prism 9.0 | GraphPad | RRID:SCR_002798 | |