Single-cell transcriptomics of a dynamic cell behavior in murine airways

Abstract
Data availability
Article and author information
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Abstract

Despite advances in high-dimensional cellular analysis, the molecular profiling of dynamic behaviors of cells in their native environment remains a major challenge. We present a method that allows us to couple physiological behaviors of cells in an intact murine tissue to deep molecular profiling of individual cells. This method enabled us to establish a novel molecular signature for a striking migratory cellular behavior following injury in murine airways.

Data availability

Sequencing data have been deposited in GEO under accession code GSE193954.

The following data sets were generated

1. Kwok SJ
2. Montoro DT
3. Haber AL
4. Yun S
5. Vinarsky V
(2022) Single-cell transcriptomics of dynamic cell behaviors
NCBI Gene Expression Omnibus, GSE193954.

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE193954

The following previously published data sets were used

1. Plasschaert LW
2. Zilionis R
3. Choo-Wing R
4. Savova V
5. Knehr J
6. Roma G
7. Klein AM
8. Jaffe AB
(2018) A single cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte
NCBI Gene Expression Omnibus, GSE102580.

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE102580

Article and author information

Author details

Sheldon JJ Kwok

LASE Innovation Inc, Cambridge, United States

Competing interests
Sheldon JJ Kwok, Currently an employee of and has financial interests in LASE Innovation Inc..
Daniel T Montoro

Broad Institute, Cambridge, United States

Competing interests
No competing interests declared.

"This ORCID iD identifies the author of this article:" 0000-0002-6222-2149
Adam L Haber

Broad Institute, Cambridge, United States

Competing interests
No competing interests declared.
Seok-Hyun Yun

Wellman Center for Photomedicine, Harvard Medical School, Cambridge, United States

For correspondence
seok-hyun_yun@hms.harvard.edu

Competing interests
Seok-Hyun Yun, Has financial interests in LASE Innovation Inc. that were reviewed and are managed by Massachusetts General Hospital and Mass General Brigham in accordance with their conflict-of-interest policies..
Vladimir Vinarsky

Center for Regenerative Medicine, Massachusetts General Hospital, Boston, United States

For correspondence
vvinarsky@gmail.com

Competing interests
Vladimir Vinarsky, Currently an employee and has financial interest in Vertex Pharmaceuticals, Inc..

"This ORCID iD identifies the author of this article:" 0000-0003-1141-6434

Funding

National Heart, Lung, and Blood Institute (5P01HL120839)

Seok-Hyun Yun

National Heart, Lung, and Blood Institute (5F32HL154638)

Daniel T Montoro

National Institute of Biomedical Imaging and Bioengineering (P41EB015903)

Seok-Hyun Yun

National Institute of Biomedical Imaging and Bioengineering (P41EB015903)

Seok-Hyun Yun

National Cancer Institute (R01CA192878)

Seok-Hyun Yun

National Heart, Lung, and Blood Institute (K08HL124298)

Vladimir Vinarsky

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: Mice were maintained in an Association for Assessment and Accreditation of Laboratory Animal Care-accredited animal facility at the Massachusetts General Hospital, and procedures were performed with Institutional Animal Care and Use Committee (IACUC)-approved protocol 2009N000119.

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.