Single-cell transcriptomics of a dynamic cell behavior in murine airways
- LASE Innovation Inc, United States
- Broad Institute, United States
- Harvard Medical School, United States
- Massachusetts General Hospital, United States
Abstract
Despite advances in high-dimensional cellular analysis, the molecular profiling of dynamic behaviors of cells in their native environment remains a major challenge. We present a method that allows us to couple physiological behaviors of cells in an intact murine tissue to deep molecular profiling of individual cells. This method enabled us to establish a novel molecular signature for a striking migratory cellular behavior following injury in murine airways.
Data availability
Sequencing data have been deposited in GEO under accession code GSE193954.
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Single-cell transcriptomics of dynamic cell behaviorsNCBI Gene Expression Omnibus, GSE193954.
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A single cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyteNCBI Gene Expression Omnibus, GSE102580.
Article and author information
Author details
Funding
National Heart, Lung, and Blood Institute (5P01HL120839)
- Seok-Hyun Yun
National Heart, Lung, and Blood Institute (5F32HL154638)
- Daniel T Montoro
National Institute of Biomedical Imaging and Bioengineering (P41EB015903)
- Seok-Hyun Yun
National Institute of Biomedical Imaging and Bioengineering (P41EB015903)
- Seok-Hyun Yun
National Cancer Institute (R01CA192878)
- Seok-Hyun Yun
National Heart, Lung, and Blood Institute (K08HL124298)
- Vladimir Vinarsky
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: Mice were maintained in an Association for Assessment and Accreditation of Laboratory Animal Care-accredited animal facility at the Massachusetts General Hospital, and procedures were performed with Institutional Animal Care and Use Committee (IACUC)-approved protocol 2009N000119.
Reviewing Editor
- Murim Choi, Seoul National University, Republic of Korea
Version history
- Received: December 23, 2021
- Preprint posted: January 31, 2022 (view preprint)
- Accepted: April 20, 2023
- Accepted Manuscript published: April 21, 2023 (version 1)
- Version of Record published: May 11, 2023 (version 2)
Copyright
© 2023, Kwok et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Single-cell transcriptomics of a dynamic cell behavior in murine airways
eLife 12:e76645.
https://doi.org/10.7554/eLife.76645
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