(a) Latent time analysis reveals distinct gene expression patterns in adult tFOLR2-MF. Note the gradual down-regulation of MHC-related genes (for example, H2–Ab1, H2–Aa, Cd74), proliferation-associated genes (for example, Cdk1, Cenpp) and genes that are highly expressed in tCX3CR1-MF (e.g. Cx3cr1, Hexb, Axl). Early (blue) and late (red) expressed genes that were used in (b) are marked with a rectangle. (b) We used Lisa Cistrome (Qin et al., 2020) (http://lisa.cistrome.org/) to investigate public chromatin profile data from a comprehensive database of mouse H3K27ac ChIP-seq profiles to determine transcription factors that are possibly responsible for the regulation of early and late expressed tFOLR2-MF genes. Note that early expressed tFOLR2-MF genes (blue genes in (a)) show preferential binding motifs of IRF and CEBP proteins similar to tCX3CR1-MF, while genes that are expressed in more mature tFOLR2-MF are enriched for STAT3 and OGT motifs. (c) Latent time analysis of adult tCX3CR1-MF. No clear maturation pattern could be extracted. Intermediate (blue) and late (red) expressed genes that were used in (d) are marked with a rectangle. (d) Lisa analysis of intermediate and late expressed tCX3CR1-MF gene sets as shown in (c). Both intermediate and late gene data sets showed a significant enrichment for IRF, RELA, JUNB, and BATF motifs. While intermediate genes showed binding motifs for RUNX1, BCL6, and NR3C1, the late gene set was only weakly enriched for SPI1 motifs.