Significant differences in deuterium incorporation are mapped on the structure of PLC-γ1 (H335A) according to the legend for the following three states: alone versus bound to phosphorylated kinase domain of fibroblast growth factor receptor (FGFR1K) (A), alone versus in the presence of liposomes containing phosphatidylethanolamine:phosphatidylinositol 4,5-bisphosphate (90:10) (B), or alone versus bound to both pFGFR1K and liposomes (C). In addition, Figure 2—figure supplement 4 shows differences in exchange of PLC-γ1 (H335A) bound to either liposomes or pFGFR1K relative to a final state with PLC-γ1 (H335A) bound to both liposomes and pFGFR1K. Significant differences in any peptide required three specific conditions: greater than both a 5% and 0.4 Da difference in exchange at any time point (3, 30, 300, 3000, and 10,000 s), and a two-tailed, unpaired t-test of p<0.01. The #D difference for each condition is graphed below, which shows the total difference in deuterium incorporation over the entire hydrogen-deuterium exchange time course, with each point indicating a single peptide (error shown as SD [n=3]). Each circle represents the central residue of a corresponding peptide with the full deuterium exchange information for all peptides available in the source data. Individual peptides with a significant difference as defined above are colored red.